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Citations for Leica :
1 - 50 of 644 citations for C Reactive Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
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bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
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bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
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bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
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bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2023Quote: ... The frozen blocks (−25°C to -30°C) were transferred to a cryostat (CM1950, Leica Biosystems, USA) for sectioning ...
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bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
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bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Microbiology 2020Quote: ... or 20x (Leica C Plan, NA=0.3) objective and VisiView® software.
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bioRxiv - Neuroscience 2022Quote: ... and C-X3-C motif chemokine receptor 1 (CX3CR1) and imaged with a Leica SP8 Confocal microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80°C before being cut into 25 μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... and frozen in isopentane solution (−50 °C) and stored at -80 °C for later processing to 40 μm coronal sections on a freezing sliding microtome (Leica).
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bioRxiv - Neuroscience 2021Quote: ... and stored at −80 °C before being cut into 25-μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
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bioRxiv - Genetics 2023Quote: ... OCT blocks were stored at -80°C and brought up to -20°C prior to cutting (CM3050S, Leica, Wetzlar, Germany). Frozen tissue sections (14µm thickness ...
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bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
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bioRxiv - Microbiology 2019Quote: ... equipped with a digital camera (Leica DFC450 C), or directly from the roll tube ...
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bioRxiv - Systems Biology 2020Quote: ... connected to a digital camera (Leica DFC420 C) and a computer equipped with a specific software for histomorphometric analyses (Bone 3.5 ...
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bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
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bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
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bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
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bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
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bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
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bioRxiv - Developmental Biology 2021Quote: ... -120 °C (BAF 60, Leica Micro-systems, Vienna, Austria) and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100 ...
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bioRxiv - Microbiology 2023Quote: ... The samples were cryosectioned (Leica CM 1860, −25°C) until a substantial amount of the frozen body and tentacles were exposed ...
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bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Infiltrated tissues were then casted into molds together with liquid paraffin (65°C) and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5 μm sections using a Thermo Scientific™ HM 355S Automatic Microtome and mounted on Surgipath® X-tra® Microscope Slides (Leica Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... which was set at -90 °C (EM AFS2, Leica Microsystems). Using pre-cooled tweezers ...
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bioRxiv - Neuroscience 2019Quote: ... with 40x water immersion objective (C PL APO CS2, Leica), a 63x oil immersion objective (HC PL APO CS2 ...
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bioRxiv - Immunology 2021Quote: ... Cryosections were made using a −20°C Cryostat (Leica Biosystems), with sections being cut 7µM thick and mounted onto poly-L-lysine-coated microscope slides (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2022Quote: ... After freezing at −20°C in Tissue Freezing Medium (Leica), aphids were sectioned at 10 μm using a Leica CM1950 freezing microtome ...
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bioRxiv - Microbiology 2022Quote: ... and cooled once again to −30 °C (AFS2; Leica Microsystems). Vitrified samples were rinsed four times in pure acetone before infiltration with progressively increasing concentrations of resin (Epoxy embedding medium ...
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bioRxiv - Neuroscience 2023Quote: ... in PBS at 4°C until vibratome (VT 1000S, Leica) cutting (30 μm coronal midbrain sections) ...
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bioRxiv - Cell Biology 2023Quote: ... then sectioned with a cryostat at −25°C (Leica CM1860). The sections were transferred to a room temperature Opaque-coated slide (VWR® Superfrost® Plus Micro Slide 48311-703) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
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bioRxiv - Molecular Biology 2020Quote: ICSI was performed at 17°C using an inverted microscope (Leica) equipped with micromanipulators (Narishige ...
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bioRxiv - Plant Biology 2020Quote: ... slides were dried on a hot plate at 55°C (Leica). Glass slides were then resized with a glass knifemaker to be mounted on aluminium SEM stubs using conductive carbon tabs ...
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bioRxiv - Plant Biology 2020Quote: ... slides were dried on a hot plate at 55°C (Leica). Slides were finally mounted in a 1:1 solution of AF1 antifadent (Citifluor ...
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bioRxiv - Neuroscience 2021Quote: ... equilibrated in 30% sucrose at – 20°C using a cryostat (Leica), and stored at −80°C.
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bioRxiv - Neuroscience 2020Quote: ... brains were sectioned at -22 °C with a cryostat (Leica Microsystems) at a thickness of 35 µm ...
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bioRxiv - Bioengineering 2020Quote: ... before being embedded into paraffin wax (Leica HistoCore Arcadia H & C) and cut in sections (typically between 8-12 micrometers thick ...