Labshake search
Citations for Leica :
1 - 50 of 656 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2024Quote: ... p-AKT-S473 (Leica, NCL-L-AKT-PHOS), and DAPI using a Bond RX autostainer (Leica) ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices (250–300 µm thick) were prepared with a vibratome (Leica P-1000), placed in warm artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
-
bioRxiv - Neuroscience 2020Quote: ... For p-AKt experiments astrocytes were imaged with a Confocal Leica DMI6000 microscope (Leica) and the image analysis was done with ImageJ software (NIH ...
-
bioRxiv - Neuroscience 2022Quote: ... and slices (250–300 μm thick) were prepared with a vibratome (Leica P-1000). Slices were placed in warm artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
-
bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
-
bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Neuroscience 2021Quote: ... Expression of Myh11 or P-gP was detected using the Bond Polymer Refine Detection kit (Leica) and processed on automated immunostaining systems (the Bond RX Leica for P-gP and Myh11 ...
-
bioRxiv - Neuroscience 2023Quote: ... The frozen blocks (−25°C to -30°C) were transferred to a cryostat (CM1950, Leica Biosystems, USA) for sectioning ...
-
bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
-
bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
-
bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
-
bioRxiv - Neuroscience 2022Quote: Images of p-EIF2α/GAD67/NeuN and EIF2α/GAD67/NeuN were acquired using a confocal microscope (Leica SP5) with a 40x objective and a 1.5x digital zoom ...
-
bioRxiv - Microbiology 2020Quote: ... or 20x (Leica C Plan, NA=0.3) objective and VisiView® software.
-
bioRxiv - Neuroscience 2022Quote: ... and C-X3-C motif chemokine receptor 1 (CX3CR1) and imaged with a Leica SP8 Confocal microscope (Leica Microsystems).
-
bioRxiv - Neuroscience 2021Quote: ... and stored at −80°C before being cut into 25 μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
-
bioRxiv - Neuroscience 2020Quote: ... and frozen in isopentane solution (−50 °C) and stored at -80 °C for later processing to 40 μm coronal sections on a freezing sliding microtome (Leica).
-
bioRxiv - Neuroscience 2021Quote: ... and stored at −80 °C before being cut into 25-μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
-
bioRxiv - Genetics 2023Quote: ... OCT blocks were stored at -80°C and brought up to -20°C prior to cutting (CM3050S, Leica, Wetzlar, Germany). Frozen tissue sections (14µm thickness ...
-
bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
-
bioRxiv - Microbiology 2019Quote: ... equipped with a digital camera (Leica DFC450 C), or directly from the roll tube ...
-
bioRxiv - Systems Biology 2020Quote: ... connected to a digital camera (Leica DFC420 C) and a computer equipped with a specific software for histomorphometric analyses (Bone 3.5 ...
-
bioRxiv - Cell Biology 2024Quote: ... and QWF-T (size P)) using a 100× Plan Apo 1.47 NA oil-immersion TIRF objective (Leica Microsystems, Wetzlar, Germany). Images were captured in 5 s intervals for 20 min (unless otherwise noted ...
-
bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
-
bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
-
bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
-
bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
-
bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
-
bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
-
bioRxiv - Developmental Biology 2021Quote: ... -120 °C (BAF 60, Leica Micro-systems, Vienna, Austria) and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100 ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were cryosectioned (Leica CM 1860, −25°C) until a substantial amount of the frozen body and tentacles were exposed ...
-
bioRxiv - Neuroscience 2021Quote: ... i.p.) at P120 and P123 (after 3 days of monocular deprivation) Images were visualized using a custom Leica microscope (Leica Microsystems). Red light illumination was provided by 8 individually addressable LEDs (WS2812 ...
-
bioRxiv - Cell Biology 2022Quote: Fluorescence images were acquired on a Leica TCS-SP2 AOBS confocal laser scanning microscope with a 63×/1.40 HCX PL APO oil-immersion objective and a pinhole setting of 1.0 P AU (Leica Microsystems, Wetzlar, Germany). Images shown are maximum projections of three sequential images ...
-
bioRxiv - Cell Biology 2023Quote: ... A 680 nm short pass filter and a 560 nm long pass dichroic mirror were used to respectively reduce the laser scattering and to split the NAD(P)H and FAD fluorescence toward two hybrid photomultiplier detectors (HyD, Leica Microsystems). 460/60 nm ...
-
bioRxiv - Neuroscience 2023Quote: ... The brain was rapidly placed in the same solution and 250 µm slices were prepared with a vibratome (Leica P-1000), placed in warm sucrose-based cutting solution bubbled with 95%/5% O2/CO2 (pH 7.4) ...
-
bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...
-
bioRxiv - Neuroscience 2021Quote: ... and processed on automated immunostaining systems (the Bond RX Leica for P-gP and Myh11, and the LEICA BOND III for SMA). Pictures were acquired using a slide scanner (Lamina ...
-
bioRxiv - Cell Biology 2022Quote: ... Infiltrated tissues were then casted into molds together with liquid paraffin (65°C) and cooled to form a solid paraffin block with embedded tissue (Leica EG1150 H Paraffin Embedding Station and Leica EG1150 C Cold Plate). Paraffin-embedded WAT was sliced into 5 μm sections using a Thermo Scientific™ HM 355S Automatic Microtome and mounted on Surgipath® X-tra® Microscope Slides (Leica Biosystems) ...
-
bioRxiv - Cell Biology 2022Quote: ... which was set at -90 °C (EM AFS2, Leica Microsystems). Using pre-cooled tweezers ...
-
bioRxiv - Neuroscience 2019Quote: ... with 40x water immersion objective (C PL APO CS2, Leica), a 63x oil immersion objective (HC PL APO CS2 ...