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Citations for Leica :
1 - 50 of 876 citations for 6 CHLORO 3 METHYLPYRIDO 3 4 D PYRIMIDIN 4 3H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
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bioRxiv - Cell Biology 2024Quote: ... sectioned to 3-4 μm thickness (Leica RM2255, Germany), and stained with hematoxylin and eosin (H&E) ...
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bioRxiv - Neuroscience 2019Quote: ... 3 and 4 are compositions of more fields automatically generated by Leica LAS X software or NIS elements software ...
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bioRxiv - Pathology 2021Quote: ... Slides were then treated with 3-4% hydrogen peroxide (Leica Biosystems RE7101) for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... specimens were sputter coated with 3-4 nm of platinum (Leica EM ACE600). Samples were imaged using a Helios Nanolab 660 DualBeam Microscope (FEI).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome ...
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bioRxiv - Molecular Biology 2022Quote: ... Green siliques were harvested 12 d after flowering from 4-6 week old plants and opened using fine needles under a dissecting microscope (Leica MZ9.5, Germany). Images of opened siliques were captured using a Leica DFC290 (Germany ...
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bioRxiv - Physiology 2023Quote: ... Sections of 4-6 µm were produced using a microtome (Leica). Deparaffination and rehydration was performed using standard procedure by incubating in Xylene (2 x 5 min) ...
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bioRxiv - Immunology 2022Quote: ... 3′-3-diaminobenzidine (DAB) substrate (Leica Microsystems) was used as a chromogen followed by hematoxylin counterstain ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in agar (3-4%) and coronal sections (50 µm) were cut on a vibrating microtome (Leica VT1000S) and collected in a 1 in 4 series ...
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bioRxiv - Cell Biology 2019Quote: PFA-fixed adult mouse (3-4 month) brains were cut into 1-mm-thick sections using a vibratome (Leica, VT1200S) [11 ...
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bioRxiv - Cell Biology 2021Quote: ... Dried samples were then coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The sample grids were imaged using a JEOL JEM2800 scanning/transmission electron microscope at 200 kV.
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bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
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bioRxiv - Plant Biology 2022Quote: ... The samples were then coated with 3 nm platinum and 4 nm carbon using the ACE600 coating device (Leica Microsystems).
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bioRxiv - Cell Biology 2021Quote: ... whereas samples for STEM were attached to a sticky Post-It-Note (as described in (31)) and coated with 3 nm platinum and 4 nm carbon using an ACE600 coating device (Leica Microsystems). The STEM samples were then washed with Buffered Oxide Etchant (diluted 6:1 with surfactant ...
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bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
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bioRxiv - Cell Biology 2024Quote: ... The tissues in paraffin blocks were subjected to sectioning (3-4 μM) by rotary type of microtome (Leica RM2255 Rotary Microtome, Leica Biosystems). The tissue sections were fixed in a glass slide and stained with hematoxylin and eosin (H&E ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... blotting for 3-seconds at 4 °C with 90% humidity and plunging into liquid ethane using a Lecia EM GP device (Leica Microsystems, Germany). Cryo-EM data were collected on a Talos Arctica transmission electron microscope operated at 200 kV acceleration voltage using EPU automated data collection software (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
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bioRxiv - Immunology 2019Quote: Pools of 20 larvae were collected at 3- and 4-day post infection (dpi) and imaged by using the Leica MZ16FA Fluorescence Stereo Microscope (Leica Microsystems, Wetzlar Germany) equipped with the DFC420C color camera (Leica Microsystems) ...
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bioRxiv - Developmental Biology 2020Quote: Imaging of embryos and tissues was performed using a Leica MZ 16F dissecting microscope equipped with a Leica DFC420 C digital camera supported by the Leica Application Suite software package (LAS, version 3 or 4; Leica Microsystems, Germany/Switzerland), and Leica EL6000 fluorescence light source.
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bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
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bioRxiv - Bioengineering 2022Quote: ... 3′-diaminobenzidine (Leica).
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bioRxiv - Neuroscience 2019Quote: ... unfixed C57BL/6 mouse brains (N = 3) were embedded in tissue freezing medium (Leica Biosystems, Richmond, UK), frozen on a dry ice ethanol bath ...
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bioRxiv - Developmental Biology 2023Quote: ... was injected into the cytoplasm of wild-type embryos at one-cell stage using an MPPI-3 microinjector (ASI) under a stereomicroscope (S9E, Leica). Injected embryos were incubated in the embryo medium (10% Hank’s with full strength calcium and magnesium ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Neuroscience 2021Quote: ... Mice were anesthetized with a mixture of oxygen (1L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
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bioRxiv - Biochemistry 2022Quote: ... Pelco Easiglow) and vitrified (3 μL sample, 3 s blot time) using an automatic plunge freezer (Leica). EndoX-TSEN•CLP1 was frozen on R1.2/1.3 Cu 300 mesh grids (Quantifoil ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were fixed 6 hours and 3 days after seeding and imaged in brightfield using a Leica DMI 4000 B microscope (Leica Microsystems).
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bioRxiv - Neuroscience 2022Quote: ... Frozen brains were cut to either 10 μm slices for immunofluorescence staining or 4-6 μm for STORM (CM1950, Leica) to produce coronal brain sections.
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bioRxiv - Cell Biology 2021Quote: ... Micro 3 - a DMi8 microscope (Leica Microsystems) with a high-contrast Plan Apochromat 63×/1.30 Glyc CORR CS objective (Leica Microsystems) ...
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bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
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bioRxiv - Neuroscience 2020Quote: ... containing dorsal hippocampus (from 3, 6, 9, 12, 15, 19, and 24 month-old) were cut from PFA-fixed hemispheres (Leica vibratome VT1000P) and stored in 0.1M phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Permeabilized cells were probed for SMAD2/3 (#AF3797, R&D Biosystems, 10μg/mL) and imaged on an SP8 white laser confocal microscope (Leica Biosystems, Wetzlar, Germany). The following antibodies were used for additional immunofluorescence assays ...
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Developmental Biology 2021Quote: ... GV-stage oocytes were injected with 10 pl of a solution containing 0.1 mM Emi2-3’UTR-MO or Emi2-3’UTR 5mm-MO using an IM-9B microinjector (Narishige) under a Dmi8 microscope (Leica) in M2 medium ...
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bioRxiv - Molecular Biology 2020Quote: ... the individual primordial oocyte was collected from 2-3 adjacent tissue sections to ensure that one full cell was included (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Microbiology 2020Quote: ... coated with 3 nm gold/palladium (Leica CPD300) and examined with a JEOL JSM-7400F scanning electron microscope (3 kV-LEI detector).
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bioRxiv - Neuroscience 2023Quote: ... stained with 3% lead citrate (Leica UltroStain II) for 3 m ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...