Labshake search
Citations for Leica :
1 - 50 of 1955 citations for 6 4 Methyl 1 piperazinyl N 5 methyl 1H pyrazol 3 yl 2 1Z 2 phenylethenyl 4 pyrimidinamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... Cell counts were done on serial sections immunostained for GnRH-1 at E13.5 (n=3) and E15.0 (n=4) and visualized under bright field (immunoperoxidase) or epi-fluorescence illumination (20×; Leica DM4000 B LED), according to their anatomical location [i.e. ...
-
bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
-
bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
-
bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
-
bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
-
bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
-
bioRxiv - Developmental Biology 2020Quote: ... Image stacks were obtained every 3-4 minutes for 6-8 hours using a LSM880 (Leica) with an environmental chamber (37°C with 5% CO2) ...
-
bioRxiv - Cell Biology 2023Quote: ... the tibiae were embedded in methyl methacrylate (MMA) and 5 µm sections cut with a tungsten steel knife on a motorised rotary microtome (Leica). Sections were stained either with Goldner’s Trichrome to analyse osteoid and lipid droplets or counterstained with calcein blue to quantify the amount of bone formed between the injected alazarin red-S and calcein ...
-
bioRxiv - Neuroscience 2023Quote: ... and immobilized in 5% methyl cellulose for imaging with a M165 FC stereo microscope and LAS Software V4.21 (Leica Microsystems). Total body length measurements and eye area were quantified using Fiji/ImageJ software (63) ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
-
bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
-
bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Neuroscience 2023Quote: ... and 3% lead citrate (Ultrostain 2, Leica).
-
bioRxiv - Cell Biology 2024Quote: ... sectioned to 3-4 μm thickness (Leica RM2255, Germany), and stained with hematoxylin and eosin (H&E) ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
-
bioRxiv - Physiology 2023Quote: ... Sections of 4-6 µm were produced using a microtome (Leica). Deparaffination and rehydration was performed using standard procedure by incubating in Xylene (2 x 5 min) ...
-
bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
-
bioRxiv - Cell Biology 2020Quote: ... Bright field images of beating cilia were captured at 160 frames per sec over 4 seconds (3-5 videos per insert, Leica Spinning disc confocal with 40x water objective) ...
-
bioRxiv - Neuroscience 2019Quote: ... unfixed C57BL/6 mouse brains (N = 3) were embedded in tissue freezing medium (Leica Biosystems, Richmond, UK), frozen on a dry ice ethanol bath ...
-
bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
-
bioRxiv - Cell Biology 2019Quote: ... for 2 days at 4 °C and then transferred to 70% alcohol for 3-5 days before processed by using 4-hour tissue processing setting with Leica ASP6025 (Leica Biosystems). Placentas were embedded in paraffin (HistoCore Arcadia H and Arcadia C ...
-
bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
-
bioRxiv - Neuroscience 2019Quote: ... 3 and 4 are compositions of more fields automatically generated by Leica LAS X software or NIS elements software ...
-
bioRxiv - Pathology 2021Quote: ... Slides were then treated with 3-4% hydrogen peroxide (Leica Biosystems RE7101) for 10 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... or for 4–6 s at 4°C and ~ 86% and plunge frozen using a Leica EM GP (Leica Microsystems Inc, Buffalo Grove, IL). The samples were imaged in an FEI Titan Krios electron microscope operated at 300 kV with a K2 Summit camera and energy filter (Gatan ...
-
bioRxiv - Neuroscience 2020Quote: ... Slices were prepared in oxygenated (95% O2 / 5% CO2) dissection artificial cerebrospinal fluid (ACSF) at low temperature (3-4°C) using vibroslicer (Leica, VT 1200S). After preparation slices were recovered in a resting chamber (Harvard apparatus ...
-
bioRxiv - Neuroscience 2019Quote: ... and kynurenic acid 1 for 2-3 min and then glued on a vibratome VT1000S (Leica). The slicing chamber was filled with the same ice-cold aCSF ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
-
bioRxiv - Cell Biology 2023Quote: ... specimens were sputter coated with 3-4 nm of platinum (Leica EM ACE600). Samples were imaged using a Helios Nanolab 660 DualBeam Microscope (FEI).
-
bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
-
bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
-
bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
-
bioRxiv - Neuroscience 2022Quote: ... 1-hour each), xylene (3×, 1.5-hour each), paraffin (3 ×, 2-hours each) (Fisher, UK) in tissue processor (Leica TP1020 tissue processor, UK) and embedded in paraffin using embedding station (Leica HistoCore Arcadia H embedding station ...
-
bioRxiv - Cell Biology 2019Quote: PFA-fixed adult mouse (3-4 month) brains were cut into 1-mm-thick sections using a vibratome (Leica, VT1200S) [11 ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... About 3-4 µm sections were prepared using an automated microtome (RM2255, Leica Biosystems, Germany) and the sections were stained with hematoxylin and eosin (H&E ...
-
bioRxiv - Cell Biology 2022Quote: ... Regions 2 and 3 were then further sectioned using a vibratome (VT1000S, Leica) set to produce 200 μm sections ...
-
bioRxiv - Biochemistry 2023Quote: ... and coated by sputtering of 2-3 nm of Chromium (ACE600, Leica microsystems) prior to observation with a high-resolution Field-Emission SEM operating at 1.5 kV (GeminiSEM500 ...
-
bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 Nucleoprotein: 1:2000 (Leica, AR9961) 30minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell nucleus was defined and exposed to 405 nm laser light for 4-5 times 200ms bursts at ∼5% 405 nm laser power (TCS SP8 MP, Leica). After photoconversion of Dendra2-RUVBL2 ...
-
bioRxiv - Bioengineering 2020Quote: ... with 2 to 3 sections placed onto Leica X-tra adhesive microscope slides (Leica Biosystems Nussloch GmbH ...
-
bioRxiv - Developmental Biology 2019Quote: ... Serial sections of 4–6 µm thickness were cut using a Leica RM2255 rotary microtome (Leica Microsystems GmbH, Germany) with disposable blades ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed in BOND Epitope Retrieval Solution 1 (pH 6) or 2 (pH 9) (ER1, ER2, Leica Biosystems, Cat. AR9961, AR9640) at 95°C ...
-
bioRxiv - Biochemistry 2020Quote: ... and allowed to set O/N at 4 °C before being imaged using a SP8 white-laser confocal microscope (Leica Microsystems). Filters were adjusted specifically for each stain to visualize nuclei (Hoechst ...