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Citations for Leica :
1 - 50 of 107 citations for 2x SYBR Green qPCR Master Mix Low ROX since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... A low fluorescence immersion oil (11513859; Leica) was used ...
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bioRxiv - Cell Biology 2022Quote: ... low-profile microtome blades (Leica 819, #14035838925), and TruBond™ 380 adhesion slides (Electron Microscopy Sciences ...
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bioRxiv - Neuroscience 2019Quote: ... low-Na+ saline using a vibratome (VT1200S, Leica), incubated at 32°C for 20–40 min in normal artificial cerebrospinal fluid (aCSF ...
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bioRxiv - Neuroscience 2020Quote: ... in a low-temperature embedding system (Leica, EM AFS2). The embedded tissue with a block face size of 300 μm × 400 μm was serially sectioned at 85 nm with an ultramicrotome (RMC products ...
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bioRxiv - Immunology 2022Quote: ... and ddH2O (2x) with a Leica ST4020 Linear Stainer (Leica Biosystems) programmed for 30 s each ...
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bioRxiv - Neuroscience 2023Quote: Frozen samples were embedded at low temperature (AFS2; Leica Microsystems). They were first exposed to 0.1% tannic acid in acetone ...
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bioRxiv - Biochemistry 2023Quote: ... a low magnication of 10X using simple microscope (Leica DM500) was utilized.
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bioRxiv - Molecular Biology 2023Quote: ... and 50 mM Tris-HCl pH 8 in 2X SSC) and imaged by Leica TCS SP8 confocal microscope with 60× water lens (NA 1.2) ...
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bioRxiv - Plant Biology 2023Quote: ... photographed at low magnification (0.75×) under a dissecting microscope (Leica MZFLIII, Wetzlar, Germany), and their areas measured with the ImageJ software (Rasband ...
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bioRxiv - Neuroscience 2021Quote: ... 2x 100% Xylol) followed by paraffinization on the STP 120 tissue processing machine (Leica microsystems). Samples were retrieved and embedded in paraffin blocks on the HistoStar embedding workstation (Epredia) ...
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bioRxiv - Neuroscience 2020Quote: The frozen samples were transferred to a low temperature embedding device (ASF2; Leica Microsystems) and into 0.1 % tannic acid in acetone for 24 hr at −90° C ...
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bioRxiv - Plant Biology 2023Quote: ... Low magnification imaging with a 10X objective (HC PL Fluotar, NA 0.3, Leica, Germany) was used to characterise algal colony size throughout the agarose hydrogel matrix volume by performing a z-stack across varied depth levels from the glass bottom interface to the top of the gel surface ...
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bioRxiv - Neuroscience 2023Quote: ... molded into low-melt agarose and mounted in a Leica VT1200s vibratome (Leica Biosystems). Mouse retina slicing solution was continuously bubbled with 100% O2 and contained the following ...
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bioRxiv - Developmental Biology 2020Quote: ... distilled water using a MZ205 FA fluorescent dissection microscope with a 2x PLANAPO objective lens (Leica) and LasX software (Leica) ...
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bioRxiv - Neuroscience 2019Quote: ... the brain was sliced (approximately 33 μm sections) with a low-temperature Cryostat (Leica Biosystems) and pasted on cover glass ...
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bioRxiv - Genetics 2022Quote: ... brains were embedded in 3% low-melting agarose and cut with a vibratome (VT1200S, Leica). 100µm thick sections were mounted on gelatine-coated slides and allow to dry for two days before staining ...
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bioRxiv - Bioengineering 2023Quote: SNO were embedded in 3% low melting agarose and sectioned using Leica VT1000S vibratome (Leica) into slices of 400 μm diameter ...
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bioRxiv - Developmental Biology 2023Quote: ... 4% low-melting point (LMP) agarose or OCT and sectioned at 50µm using a vibratome (Leica) or at 15µm using a cryostat (Microm) ...
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bioRxiv - Neuroscience 2021Quote: ... Image stacks were acquired every 10 seconds at a scan speed of 1400 Hz with 2X line averaging using LAS AF 2.7.3 (Leica).
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bioRxiv - Developmental Biology 2019Quote: ... Images were acquired with a low-magnification water immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). For four-dimensional imaging ...
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bioRxiv - Developmental Biology 2019Quote: ... Images were acquired with a low-magnification water immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). The optical plane was moved 2 μm between the z-sections until the whole OFT was acquired ...
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bioRxiv - Plant Biology 2020Quote: ... embedded in 5% low-melting point agarose and cutted using a vibratome (VT1000S; Leica, Rueil-Malmaison, France) as described in (Djébali et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Low magnification bright field or DIC time-lapse acquisition was carried out using inverted routine microscopes (Leica), equipped with PAL cameras (Prosilica ...
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bioRxiv - Cell Biology 2020Quote: ... EV internalization were visualized through the Z-plane at 0.1μm increments under 63X oil immersion and 2X or 5X digital zoom (Leica TSP8). Line scan analyses were collected using Fiji software (ImageJ ...
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bioRxiv - Biophysics 2020Quote: ... Cy3 fluorescence was excited with a green laser (532 nm, Leica), and emitted photons were captured through an objective lens (63× ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed tissue was sectioned either by embedding in low melting point agarose and sectioning on vibratome (Leica VT1000S) into 100-150µm sections or cryopreserved in OCT(6502 ...
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bioRxiv - Developmental Biology 2019Quote: ... Images were acquired bidirectionally with a low-magnification water immersion objective (Leica HCX IRAPO L, 25X, N.A. 0.95). For time lapse ...
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bioRxiv - Plant Biology 2023Quote: ... embedded in 4% low melting agarose and sectioned into 150 µm cross sections using the vibratome VT1000s (Leica). Images were acquired immediately after sectioning on the LSM800 using a 63.0 x oil immersion objective (Zeiss) ...
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Glia detect and mount a protective response to loss of dendrite substructure integrity in C. elegansbioRxiv - Neuroscience 2023Quote: Animals were anesthetized on ice for ~10-60 minutes prior to observation with a fluorescence dissecting microscope equipped with a 2X objective (Leica). Animals with obvious GFP accumulation at the nose tip were scored as strong ...
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bioRxiv - Developmental Biology 2023Quote: ... fixed and stained aggregates were resuspended in low melting agarose and placed in 1.5 mm U-shaped capillaries (Leica). Capillaries were placed into water filled 35mm high glass bottom µ-dishes (ibidi) ...
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bioRxiv - Systems Biology 2023Quote: Low density polyethylene (LDPE) fibers (GoodFellow, LS554234) were cut to 50 µm using a cryotome (Leica biosystem 1950 cryotome) protocol for preparing microplastic fibers (29) ...
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bioRxiv - Cell Biology 2019Quote: ... Low-magnification bright field images of spheroids were also acquired using a tissue culture inverted microscope (DM IL, LEICA Microsystems), with a universal smartphone adapter (Modern Photonics ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 300µm-thick acute coronal brain sections were cut after embedding in 4% low melt-agar using a vibratome (Leica, #VT1000S) under RNase-free conditions ...
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bioRxiv - Neuroscience 2020Quote: ... brains were embedded in a 5% low-gelling agarose gel (in PBS) and 50µm coronal slices were cut using a vibroslicer (Leica VT1000S). Slices were then stored in PBS-Azide (0.02-0.05% ...
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bioRxiv - Neuroscience 2020Quote: ... the slices were embedded in 4% low melting agarose and cut in 70µm thick sections in a vibrating microtome (Leica, VT1000S). Sections around the tip of the recording electrode were collected and cryoprotected in 10% and 20% sucrose solutions in PB ...
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bioRxiv - Neuroscience 2020Quote: ... Spinal cords were removed from the spinal column by hydraulic extrusion and embedded in low-melting point agarose (2-3%) before sectioning using a vibratome (Leica) into 300 µm thick coronal slices in ice-cold oxygenated ACSF ...
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bioRxiv - Neuroscience 2019Quote: ... The OE were embedded in 4% low melting point agarose blocks and sectioned (30 µm) using a Vibratome (VT 1200; Leica). The sections were blocked in 1% blocking solution (10 min at RT ...
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bioRxiv - Biophysics 2020Quote: ... deeply anesthetized fish larvae with immobilized hearts were embedded in 1% low-melting agarose for sustained confocal imaging (SP8-STED/FLIM/FCS, Leica) using a 20×/0.75 Objective (HC PL APO CS2) ...
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bioRxiv - Neuroscience 2021Quote: ... The cortices were removed and then coronal midbrain slices (250 μm) were cut in ice-cold oxygenated low-calcium aCSF on a vibratome (Leica VT1200S and vibrating microtome 7000smz ...
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bioRxiv - Developmental Biology 2021Quote: ... Fixed heads were embedded in 4% low gelling agarose and 200 µm sections were obtained using a vibratome (Leica, VT1200S). Sections were post-fixed with 1% osmium tetroxide in water ...
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bioRxiv - Neuroscience 2022Quote: ... embedded into 3% low melting point agarose and then sectioned into 50-60 um using a vibratome (Leica, cat# VT1000S), mounted for direct visualization of EGFP or processed for immunofluorescence as it follows ...
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bioRxiv - Neuroscience 2023Quote: ... adult brains were fixed in 4% PFA and included in 7% low-melting agarose in PBS 1X and sectioned at 100 µm using a vibratome (Leica). Sections were mounted with Glycergel (Dako ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Food carpets were dried for ∼12 hrs in a low humidity room and imaged under a light stereomicroscope (Leica M165FC) at 32x magnification (Fig ...
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bioRxiv - Neuroscience 2023Quote: ... Confocal z-stacks covering the whole depth of the sections were taken with 0,38 μm step size (63x oil objective and 2x digital zoom magnification) and a laser scanning confocal microscope (Leica TCS SPE, Germany). Only confocal planes with the optimal penetration level for each antibody were selected ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... Detached nodules were embedded in 5% low melting point agarose and sliced into 30-μm thick sections with a rotary microtome RM2235 (Leica Microsystems). Roots were also stained with X-Gal (Affymetrix ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and coronal cortical slices were generated (300 µm) with a vibrating microtome (VTS1000 Leica Microsystems). Slices were next incubated for 60 minutes at 37°C in DMEM/F-12 imaging media without phenol red supplemented with 10% fetal bovine serum (FBS).
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bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
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bioRxiv - Neuroscience 2020Quote: ... Sections of 300μm thickness were embedded into 4% low melting agarose and further cut into 50 μm slices with vibrating-blade microtome (Leica, Germany). Nuclei were counterstained by adding a small drop of ProLong™ Gold Antifade Mountant with DAPI (Invitrogen™ ...
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bioRxiv - Neuroscience 2020Quote: Brain organoids (4.5 months old) were embedded into 4% low melting agarose and 300 μm slices were cut with vibrating-blade microtome (Leica, Germany). Slices were prepared using ice-cold artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Immunology 2022Quote: ... Larvae were then immediately mounted in 1% low-melting agarose containing 40 μg/ml tricaine and live imaged overnight using confocal microscopes (Zeiss 780 NLO or Leica SP8). The fluorescence intensity over time was calculated using SUM intensity projections ...