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Citations for Leica :
351 - 400 of 525 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The following antibodies were used at the indicated dilution and indicated antigen retrieval method: Ki67 (CST 12202, 1:1000, ER2 Leica), CD8a (eBioscience 14-0808-82 ...
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bioRxiv - Cell Biology 2024Quote: ... The embedded tissues were flash-frozen in liquid nitrogen for 1 minute and then sectioned into 6 μm-thick slices using a frozen microtome (Leica). The sections were stained with hematoxylin and eosin (Servicebio ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear DNA was stained using Hoechst 33258 (1:10,000, Pierce) Brain sections - Post fixed brains were sectioned via vibratome (Leica VT1200) at 100 µm ...
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bioRxiv - Microbiology 2024Quote: ... Quantifoil grids consisting of a 1.2/1.3 μm holey carbon film on 300 gold mesh were glow discharged at 15 mA for 1 minute with an EM ACE200 glow discharger (Leica). Just before vitrification ...
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bioRxiv - Developmental Biology 2023Quote: ... dehydrated in methanol and cleared in Benzyl Alcohol/Benzyl Benzoate (1/2) prior to mounting and imaging with a SP8X confocal microscope (Leica). A 3D binary mask was built using Fiji to obtain a binary image of the tissue ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored in a 4% formaldehyde and 30% sucrose solution for at least 1 week before slicing with a freezing microtome (Leica) at 50uM ...
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bioRxiv - Neuroscience 2023Quote: ... the animals were placed under 0.8-1% isoflurane anesthesia (2% used for induction) and imaged on an upright laser-scanning confocal (Leica SP8) or upright laser-scanning two-photon microscope (Bruker Ultima ...
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bioRxiv - Immunology 2023Quote: ... images were acquired with z-stacks between 10-15 pm (z-steps: 1 pm) and 3D-reconstructed and displayed in ‘Maximum’-mode using the Leica Application Suite X software (Leica).
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bioRxiv - Microbiology 2022Quote: ... Cells were kept in serum- and phenol red-free MEM containing 1% glucose during the analysis (Leica Biosystems 6000B microscope). A total of 15 random snapshots (55-70 cells in total ...
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bioRxiv - Immunology 2022Quote: Imaging was performed on an inverted Leica TCS SP8 confocal microscope with white light laser and HyD photodetectors using an HC PL APO CS2 20 × /0.75 IMM objective (zoom factor of 1) (Leica Microsystems). Images were deconvolved using the LIGHTNING module in Leica Application Suite X (Leica Microsystems) ...
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bioRxiv - Cancer Biology 2022Quote: ... with antigen retrieval at 100ºC for 20 min with Epitope Retrieval Solution 1 or 2 respectively (AR9961, AR9640, Leica Biosystems). TMAs were blind-scored by multiplying staining intensity with staining coverage ...
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bioRxiv - Cell Biology 2022Quote: ... the pulp chambers of the mandibular first molars were opened by a high-speed handpiece with #1/4 carbide round bur under direct visualization by a stereoscopic microscope (Leica). A #10 endodontic K file was then used to probe the root canals ...
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bioRxiv - Plant Biology 2022Quote: ... Seedlings with the typical mdf-1 phenotype were transferred to a new plate to be observed under the fluorescent stereo microscope (Leica M165 FC Fluorescent stereo microscope equipped with a Leica DFC 420C camera ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then subjected to heat induced antigen retrieval and stained for anti-phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) and anti-KI-67 (CST #9027, 1/400 in PBS/2% BSA) on bond RX (LEICA). Appropriate negative and positive controls and isotype controls were included ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope antigen retrieval was performed at 100°C for 20 min in Bond Epitope Retrieval Solution 1 (Cat#AR9961, Leica) for GRPR or Bond Epitope Retrieval Solution 2 (1/100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated in anti-GRPR antibody (SP4337P, Acris Antibodies) in Bond Primary Antibody Diluent (1/100, Cat# AR9352, Leica) overnight at 4°C and anti-Ecad (1/100 ...
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bioRxiv - Biochemistry 2023Quote: ... In the first channel, a 1-ml syringe tip (Braun, 9166017V) was used to connect the perfusion needle (Leica, 39471024) and the reference tube (Ismatec Reglo ...
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bioRxiv - Developmental Biology 2024Quote: ... and whole-mounted mouse kidney explants were performed using (1) Leica SP8-X confocal fluorescence imaging system (Leica Microsystems, Germany), in 1024 x 1024 pixels using 25X water ...
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bioRxiv - Neuroscience 2024Quote: Z-stacks of zebra finch SETBP1 and co-localization experiments in Area X were acquired with a SP8-1 confocal microscope (Leica). All microscope settings were kept constant for all conditions and slices ...
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bioRxiv - Neuroscience 2024Quote: ... Z-stack were taken at 1 μm intervals using a 40x objective lens (W CORR CS2, NA: 1.10, Leica, Germany) on the Stellaris8 microscope (Leica ...
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bioRxiv - Developmental Biology 2024Quote: ... Included tendon constructs were then frozen at -80°C in isopentane for 1 minute and 12µm cryosections were generated with a CM3050 S Cryostat (Leica Biosystems).
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bioRxiv - Microbiology 2024Quote: ... Washed cell pellets were resuspended in a total volume of 50 µl PBS and 10 µl of each sample was spotted onto 1 % agar pads and subsequently imaged using the Thunder imager 3D cell culture microscope (Leica). First ...
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bioRxiv - Physiology 2024Quote: ... and covered with #1 thickness cover glass (Fisherbrand, cat#12-545-F) using a histological automaton (Leica Biosystem, cat#ST5020). Negative controls were treated with 1% BSA in PBS without the primary or secondary antibodies.
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled GFAP and EM48 fluorescent images were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Blinded images were opened in FIJI/ImageJ and condensed using Z project feature at maximum intensity ...
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bioRxiv - Neuroscience 2024Quote: ... 20x tiled EM48 fluorescent images from the striatum were acquired with a 16 μm z-dimension and 1 μm step size on a confocal microscope (Stellaris, Leica). Images were blinded ...
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bioRxiv - Neuroscience 2024Quote: ... and then a 1-mm-thick coronal slice containing the entire SCN was prepared using a vibratome (VT 1200S, Leica). Following this ...
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bioRxiv - Bioengineering 2024Quote: The specimens were immersed in an external cryoprotectant solution (1-hexadecene) and gently placed into HPF sample carriers (depth: 0.2 mm, Type A, Leica 16770141). The cover (Type B ...
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bioRxiv - Cell Biology 2024Quote: ... ZO-1 and Claudin-5 were acquired using a Leica SP-8 HCX PL APO CS at 63× magnification (Leica) or a DMI-8 TIRF microscope at 20× magnification (Leica Microsystems) ...
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bioRxiv - Cell Biology 2024Quote: ... and EB3 comets in single cells were imaged for 60 seconds (1 frame per 2 seconds) with a Leica DMi8 microscope (Leica). Only cells exhibiting both tdTomato and EYFP signals were included in the analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... Briefly, MHC-II (clone V1030, dilution 1:500; Biomeda, Foster City, CA, USA) was revealed using Novolink Polymer (Leica Biosystems). Slides were then decolored using ethanol and the previous antibody was stripped using a β-mercaptoethanol/SDS solution in a waterbath preheated at 56°C ...
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bioRxiv - Cancer Biology 2024Quote: ... The following antibody was used: anti CD31 (CST, 3528, clone 89C2, 1:1600, antigen retrieval Leica ER1 AR9961 for 30min).
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bioRxiv - Cell Biology 2020Quote: ... Samples were mounted laterally in 1% agarose and imaged with a 10x objective lens on a SP5 confocal microscope (Leica Microsystems).
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bioRxiv - Immunology 2021Quote: Grids were screened for cells with ASC/caspase-1 speck by light microscopy using a cryo-FM microscope (Leica EM Cryo CLEM, Leica Microsystems), equipped with a cryo-stage ...
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bioRxiv - Cell Biology 2022Quote: ... pre-blotted and blotted with filter paper (Whatman 1) for 2 sec followed by plunging into liquid ethane using a Leica EM GP (Leica Microsystems). The samples were stored in liquid nitrogen until data acquisition ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Animals were observed under blue-light-cut illumination by a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and recorded in an H264 compressed movie6 ...
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bioRxiv - Bioengineering 2021Quote: ... The experimental setup consisted of (1) our microfluidic device installed on a Leica inverted microscope (DMIL LED Inverted Routine Fluorescence Microscope, Leica, Germany) and imaged using a colour camera (MC170 HD ...
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bioRxiv - Cell Biology 2021Quote: ... and freeze substituted in anhydrous acetone containing 1 % OsO4/ 2% H2O for 64h using the Automatic Freeze Substitution unit (AFS; Leica Microsystems). The samples were included in Epon ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
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bioRxiv - Plant Biology 2021Quote: ... tumefaciens clones carrying the appropriate BiFC plasmids using a 1 mL needleless syringe and imaged two days post-infiltration with a Leica TCS SMD confocal microscope (Leica Microsystems) using the preset settings for YFP (Ex ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with a mixture of oxygen (1 L/min) and isoflurane 3% (Baxter AG, Vienna, Austria) and placed in a stereotactic frame (Angle One; Leica, Germany). The skin was shaved ...
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bioRxiv - Cell Biology 2020Quote: ... Interaction between swiprosin-1 and Rab21 in cells was detected using confocal microscopy (Leica TCS SP5, 63×1.4 Apo oil objective) and the number of PLA spots per 1000 µm3 was quantified using IMARIS software.
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bioRxiv - Plant Biology 2022Quote: ... seven-day-old Arabidopsis seedlings were incubated in 150 mM NaCl or 50 μM DNP for 1 hour and were then rapidly frozen with an EM ICE high-pressure freezer (Leica Microsystems).
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bioRxiv - Developmental Biology 2022Quote: ... A second planchet was placed over the first and then the sandwich was immediately frozen in a Leica EMPact 1 High Pressure Freezer (Leica Microsystems). The two halves of the planchet were then separated under liquid nitrogen to expose the tissue within the cavity ...
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bioRxiv - Developmental Biology 2022Quote: ... The fixed tails were placed in 1 × PBS solution for 2 hours and then embedded in 7% agarose gel (1 × PBS as the medium) and cut into 180 μm horizontal sections by a vibratome (LEICA VT1000S). All lizards were released at the exact location of capture in less than 4 hours.
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bioRxiv - Neuroscience 2020Quote: ... The signals of Dicer 1 and B2 Mm2 RNA and images of cells were obtained with a fluorescent microscope(DMi8, Leica Biosystems). The following probes were used:B2 Mm2 RNA probe 2 ...
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bioRxiv - Physiology 2021Quote: ... 20 larvae per tank were anaesthetized with MS-222 (50 mg l-1, Pharma Q) prior to feeding and directly photographed individually with a microscope (Leica M165C). The larvae were then frozen in liquid nitrogen and stored at −80°C until dry mass (DM ...
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bioRxiv - Neuroscience 2021Quote: ... 4 μm paraffin sections were prepared and processed on a Leica BOND Rx automated stainer using citrate-buffer based heat-induced antigen retrieval solution (Epitope Retrieval Solution 1 Bond, Leica, Germany) and DAB-based detection (Leica ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Cell Biology 2022Quote: ... oviducts were flushed with L15 + 1% FBS media under a 37°C dissecting microscope (Leica MZ10f, Leica Microsystems, Buffalo Grove, IL). The presence of a minimum of 6 embryos per female was confirmed as a benchmark to represent the average litter size ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... elegans behaviors were recorded at 20 frames/s through a macroscope with an apochromat objective lens (1 ×) (Z16 APO, Leica, Germany) and a CCD camera (1940 × 1460 pixels ...