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Citations for Leica :
301 - 350 of 664 citations for Recombinant Human C mer Proto Oncogene Tyrosine Kinase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... embedded in Moviol and stored at 4 °C until analysis using a confocal laser scanning microscope (TCS SP1, Leica)
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bioRxiv - Neuroscience 2021Quote: ... Brains were cut into 15 μm thick coronal sections at -22/-20°C using a cryostat (Leica CM 1950) and single sections were collected in a charged microscope slide in ascending order from the frontal to the occipital region starting in V1 ...
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bioRxiv - Microbiology 2021Quote: ... Trypanosomes at 1×106 parasites/mL were maintained in matrix-dependent HMI-11 medium containing 0,5% methylcellulose at 37°C and were observed under the 10x objective of an inverted DMI-4000B microscope (Leica) coupled to an ORCA-03G (Hamamatsu ...
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bioRxiv - Biophysics 2021Quote: ... equipped with an environmental chamber (Life Imaging Services) at 28°C and a HC PL APO 40x/1.10 W CS2 objective (Leica). Imaging parameters were set to acquire the whole sensor (z-stack ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were sectioned at 35 µm in the sagittal plane at -20° C using a cryostat (Leica CM3050 S). Each hemisphere was separately collected into two series for lateral sections ...
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bioRxiv - Neuroscience 2019Quote: ... brains were sectioned into 30 μm coronal slices at −20 °C using a CM 1850 cryostat (Leica, Wetzlar, Germany).
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bioRxiv - Systems Biology 2020Quote: ... Imaging of all embryos was performed at 23-25°C using spinning-disk confocal microscopes (Zeiss Axio Observer.Z1 or custom-modified Leica DM IRBE ...
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bioRxiv - Neuroscience 2019Quote: ... Coronal slices of the cerebellum (250 μm thick) were prepared at 4 °C using a vibratome (VS100, Leica, Germany). They were then gently transferred and stored in aCSF at room temperature (20–22 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were observed in 5% CO2 at 37°C using an HC PL APO 63x/1.40 oil objective (Leica). Multichannel time-lapse images were acquired in 4 fields each with 4 averages per frame in 1-min or 1.5-min intervals ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... Images were captured at room temperature (25°C) on a Leica TCS-NT confocal microscope (PL APO 40x oil objective, NA=1.25) by Leica TCS-NT software ...
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bioRxiv - Microbiology 2021Quote: ... organs were frozen at −80 °C and then sectioned at 20-μm-thick with the freezing microtome (Leica CM1950) for immunofluorescence labeling ...
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bioRxiv - Neuroscience 2022Quote: ... and mounted onto Über plus printer slides (InstrumeC) in a water bath set to 42°C (Leica Biosystems H1210). Sections were left to dry for 72 hours at room temperature prior to immunohistochemical staining.
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bioRxiv - Molecular Biology 2023Quote: ... Each specimen was frozen at - 20 °C using a Leica CM1950 cryostat (Leica CM1950; Leica Biosystems Nussloch GmbH, Germany) and trimmed until the specimen appeared on the cutting surface ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Plant Biology 2022Quote: ... followed by freeze substitution in dry acetone containing 0.4% uranyl acetate at −85°C in a freeze-substitution unit (EM AFS2, Leica). After three acetone rinses at −85°C ...
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bioRxiv - Genomics 2022Quote: ... tissue slides were baked overnight at 60°C to ensure tissue adherence to the positively charged glass slides (Leica Bond Plus Microscope slides ...
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bioRxiv - Neuroscience 2022Quote: ... frozen sapphire sandwiches were incubated acetone containing 0.1% tannic acid at –90 °C for 20 – 24 h inside an AFS machine (Leica) under constant agitation ...
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bioRxiv - Cell Biology 2024Quote: ... MDM cells were subsequently plunge frozen in liquid ethane at -183°C using a EM GP plunger (Leica Microsystems), with a set chamber humidity of 90% and temperature of 37°C ...
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bioRxiv - Neuroscience 2024Quote: ?ssembloids were imaged under environmentally controlled conditions (37°C) using a 5x objective in a confocal microscope (Leica SP8). For glutamate uncaging experiments ...
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bioRxiv - Cell Biology 2023Quote: ... Blocks were equilibrated at -20°C for 1 h prior to cutting 12-µm sections using a cryostat (Leica Biosystems Inc. ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and were kept at 4°C until being coronally sectioned (100 μm sections) with a Vibratome (VT1000S, Leica, Germany). Sections were mounted in Vectashield mounting medium containing DAPI (H1500 ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... The brains were then embedded in OCT and preserved at –80°C until cryosectioning at 60 µm (Leica CM3050). For free-floating immunostaining ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Twenty micrometer-thick coronal sections of mPFC were prepared at -15°C with the Leica CM1860 UV (Leica, Germany) and stored at -80°C until further processing ...
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bioRxiv - Neuroscience 2023Quote: ... samples were transferred under liquid nitrogen to an automated freeze substitution system at -90 °C (EM AFS2, Leica Microsystems). Using pre-cooled tweezers ...
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bioRxiv - Plant Biology 2023Quote: ... Grids were blotted for 2.5 s at 10 °C and 95 % humidity using a Leica EM GP plunger (Leica). Data for NCPs was acquired at the Cryo-EM facility of the Gene Centre in Munich ...
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bioRxiv - Microbiology 2023Quote: ... Trypanosomes at 1x106 parasites/mL were maintained in matrix-dependent HMI-11 medium containing 0,5% or 1.1% methylcellulose at 37°C and were observed under the 10x objective of an inverted DMI-4000B microscope (Leica) coupled to an ORCA-03G (Hamamatsu ...
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bioRxiv - Biophysics 2023Quote: ... We cut the frozen samples in 20 µm thick serial sections at -20 C using a cryostat (Leica Biosystems), and mounted them on poly-L-lysine coated slides ...
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bioRxiv - Cell Biology 2023Quote: ... the high-pressure-frozen samples were freeze-substituted in 0.2% glutaraldehyde with 0.2% uranyl acetate in acetone at -90°C in an automated freeze-substitution device (Leica AFS). After 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
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bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
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bioRxiv - Cell Biology 2024Quote: ... equipped with environmental chamber (Life Imaging Services) at 28°C using a HC PL APO 40x/1.10WCS2 objective (Leica). Whole embryo imaging was performed with a M205FCA stereomicroscope (Leica ...
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bioRxiv - Microbiology 2023Quote: ... and inhibitor-induced effects on worm motility assessed using a stereo microscope at 10x magnification (M125 C, Leica, Germany) using the following scores ...
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bioRxiv - Cancer Biology 2023Quote: ... equipped with Mai-Tai and Insight X3 tunable lasers (Spectral Physics) and a 37 °C preheated 40X objective (NA 1.10, HC PL IRAPO, Leica). The specimens were excited at 910 nm.
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bioRxiv - Molecular Biology 2023Quote: ... Inhibitor-induced effects on worm viability were assessed using a stereo microscope at 10× magnification (M125 C, Leica, Germany) and the following scores ...
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bioRxiv - Genetics 2024Quote: ... Sections were floated onto a 45 °C water bath and mounted on Leica “X-tra” adhesive slides (Leica biosytems), drained ...
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bioRxiv - Cell Biology 2024Quote: ... Images and videos were acquired using an SP8 confocal microscope system with a 63x oil-corrected lens (C-Apochromat, numerical aperture, NA = 1.4) and Lightning deconvolution software (Leica), and processed using ImageJ version 2.9.0/1.53t (109) ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence lifetime imaging microscopy (FLIM) analysis was performed at 37°C using a confocal laser scanning microscope (SP8, Leica) equipped with a Fast Lifetime Contrast (FALCON ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were extracted and post-fixed overnight at 4°C in 4% PFA (pH = 7.2) and 40-μm coronal sections were collected using a vibratome (VT1000S, Leica). After a 15-min incubation in a 4,6-Diamidino-2-phenylindole dihydrochloride hydrate (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: Formalin-fixed paraffin-embedded (FFPE) human skin tissue sections from organotypic tissue was stained for MITF (NCL-L-MITF, Leica Biosystems, Nussloch, Germany), MelanA (NCL-L-MITF ...
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bioRxiv - Neuroscience 2020Quote: ... anti-glial fibrillary acidic protein (GFAP; mouse anti-human monoclonal antibody GA5, with bond heat-induced epitope retrieval, epitope retrieval time 10 min, PA0026; Leica Biosystems, Wetzlar, Germany); anti-ionized calcium-binding adapter molecule 1 (IBA1 ...
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bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in Optimal Cutting Temperature Compound (Tissue-Tek) and stored at -80°C before sectioning on a CM1860 UV cryostat (Leica). 6μm-thick slides were stained with TUNEL (ThermoFisher ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by a final step of fresh paraffin incubation for another 20 min at 65°C (Tissue Infiltration Medium by Surgipath available through Leica). The paraffin embedded tissue was sectioned in Richard-Allan Type 9 paraffin (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: Freshly-extracted brains were frozen in isopentane at –80°C and then cut into 10 μm sections with a CM1950 Cryostat (Leica). After cutting ...
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bioRxiv - Developmental Biology 2022Quote: ... Serial 12 µm sections were obtained by cryo-sectioning of the embedded organoid at −20 °C using a cryostat (Leica). Cryosections were permeated with in PBST at temperature for 30 min and blocked with sheep serum (Zhongshanjinqiao ...
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bioRxiv - Immunology 2022Quote: ... target cells were labeled with CMTMR at 10 µM and 1:1000 Hoechst 33342 for 30 min at 37°C, and live imaged in ibidi 8 well chamber slides (ibiTreat, #80826) by confocal microscopy (SP8, Leica) with z-stacks (0.22 µm step ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Pathology 2020Quote: ... at a working solution and incubated for 15 min at 37 °C on an automated staining platform (BOND-MAX, LEICA, Leica Biosystems Newcastle Ltd. ...
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bioRxiv - Neuroscience 2019Quote: ... Imaging was performed for 5min at 1Hz at 37°C 5% CO2 on a Leica TCS SP5 system controlled by Leica LAS AF software using HCX PL APO 63×1.40 ...
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bioRxiv - Biochemistry 2021Quote: ... 3 μl of MutS-nucleotide sample were adsorbed onto glow-discharged grids and blotted for 1 second at 80% humidity at 4°C and flash frozen in liquid ethane using an EM GP plunge freezer (Leica). All cryo-EM data was collected at the The Netherlands Center for Electron Nanoscopy (NeCEN) ...