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Citations for Leica :
201 - 250 of 1391 citations for 7 2 Benzylamino ethyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were rotary-shadowed with 2 nm of platinum and 6 nm of carbon using a high vacuum sputter coater (Leica Microsystems). The resultant platinum-replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2020Quote: ... 2.3% methanol and 1–3% H2O in Acetone) tempered at −90°C in FS device (Leica AFS2) equipped with a robotic solution handler (Leica FSP) ...
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bioRxiv - Plant Biology 2022Quote: ... 1 cm root segments were embedded in 3% agarose and sectioned using a vibratome (Leica VT1000 S) to produce 150-200 μM thickness sections ...
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bioRxiv - Biophysics 2019Quote: Live COS-7 cells expressing CCR5-mTFP1 were imaged using a SP8 X SMD confocal microscope Leica microscope from Leica Microsystems (Manheim ...
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bioRxiv - Physiology 2022Quote: Aortic roots at optimal cutting temperature were embedded in NEG-50 and cut into 7 μm thick sections using a cryostat (CM1510 S; Leica). These sections were stained with Oil Red O/hematoxylin to measure lipid depot using the protocol below ...
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bioRxiv - Neuroscience 2024Quote: ... The samples were then embedded in 7% agarose and cut into 0.3 mm sagittal sections using a vibratome (Leica VT1000s). The slices were incubated overnight at 37° C in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... adult brains were fixed in 4% PFA and included in 7% low-melting agarose in PBS 1X and sectioned at 100 µm using a vibratome (Leica). Sections were mounted with Glycergel (Dako ...
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bioRxiv - Genomics 2023Quote: ... Sections were cut to a thickness of 7 μm with a Leica HistoCore AUTOCUT Rotary Microtome (Leica Biosystems, Wetzlar, Germany) and Leica RM2245 ...
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bioRxiv - Bioengineering 2023Quote: ... Images were taken with either a fluorescent light microscope (Axio Observer 7, Zeiss, Oberkochen, Germany) (Figure 3B and 5A and B) or a confocal microscope (TCS SP5X, Leica Microsystems CMS GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... and sectioned to a thickness of 7 µm for the testis and 5 µm for the epididymis using an Ultrathin Microtome from Leica, Germany ...
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bioRxiv - Microbiology 2022Quote: ... Then tissues were sectioned to 3 μm by microtome (Leica). Next ...
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bioRxiv - Immunology 2021Quote: ... 3 x H2O) on a linear stainer (Leica Biosystems, ST4020). Epitope retrieval was performed at 97C for 10 min with the Dako Target Retrieval Solution pH 9 (Agilent ...
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bioRxiv - Biophysics 2022Quote: ... The 3D model in Figure 3 was analyzed by Leica LAS X ...
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bioRxiv - Molecular Biology 2020Quote: ... the number of colonies exhibiting correct fluorescence were counted (EGFP+ for 2-insert assembly, EGFP+/mCherry+ for 6-insert assembly) using a fluorescence stereoscope (Leica M165 FC). Accuracy was calculated as the percentage of colonies exhibiting correct fluorescence ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cancer Biology 2023Quote: ... for GRPR or Bond Epitope Retrieval Solution 2 (1/100, Cat# AR9640, Leica) for Ecad ...
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bioRxiv - Neuroscience 2023Quote: ... Average power for excitation ranged from ∼35 mW to ∼80 mW measured at the front face of the objective (CA1 imaging: Nikon 16x, 3 mm WD, 0.8 NA; DG & CA3 imaging: Leica 25x, 3 mm WD, 1.0 NA). A 512 x 796 pixel field of view (FOV ...
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bioRxiv - Bioengineering 2021Quote: ... the paraffin-embedded block tissues were sectioned at the surface with ∼7-µm thickness by a microtome (RM2235, Leica Microsystems Inc.). The thin tissue slices were stained by H&E ...
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bioRxiv - Plant Biology 2020Quote: ... The hairy roots were embedded in 7% agarose before being transversely sectioned at a thickness of 50 μm using a vibratome (Leica VT1000S, Leica, Germany). The safranin O-green staining method was used to detect the deposition and composition of lignin(Bouvier et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... temporal bones were immersed in 30% sucrose in PBS and stored at 4°C for 7 days before being cut into 14-16 micron thick sections on a cryostat (Leica CM3050). For each species ...
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bioRxiv - Biochemistry 2021Quote: ... Ultrathin (70 nm) and semi-thin (300 nm) sections were generated using a Leica EM Ultracut 7 ultramicrotome (Leica, Heerbrugg, Switzerland) and sections were post-stained using 2% uranyl acetate and lead citrate respectively ...
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bioRxiv - Immunology 2022Quote: ... they were paraffin-embedded and sectioned (7 µm thickness) on slides using a Leica RM2125 RTS microtome (Leica Biosystems, Concord, Canada). Slides were deparaffinized and washed using two rounds of toluene (T324-1 ...
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bioRxiv - Immunology 2023Quote: ... cut into 4 groups of 8 sequential 7-μm-thick sections separated by a depth of 150-μm using a Leica CM1860 UV cryostat (Leica Microsystems), and mounted as duplicates on Fisherbrand ProbeOn Plus slides glass slides (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Fresh frozen tissues were placed in OCT and sections were cut at 7 μm thickness and mounted onto membrane slides (Leica, 11600288). The slides were fixed in ice-cold 70% EtOH for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... The STG was then illuminated for 5-7 mins using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV ...
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bioRxiv - Neuroscience 2024Quote: ... The STG was then illuminated for 5 – 7 min using a Texas red filter set (560 ± 40 nm wavelength; Leica Microsystems). Complete photoinactivation was confirmed when the LP membrane potential reached 0 mV.
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bioRxiv - Plant Biology 2022Quote: ... Fixed and stained roots were mounted onto microscopic slides and cleared with chloral hydrate:glycerol:water (8:3:1 ratio) before imaging with the Thunder Imaging Systems Microscope (Leica).
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bioRxiv - Physiology 2023Quote: ... and incubated for 3 x 1 h in Xylene in an automatized system (TP1020 Tissue Processor, Leica Biosystems). Sciatic nerves were then embedded in paraffin and 5 µm sections were obtained with a microtome (Leica Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and 2% lead-citrate solution (Leica Ultrostain II ...
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bioRxiv - Developmental Biology 2020Quote: ... at least 3 transverse slices cut with a vibratome (VT1000S, Leica) at different anterior-posterior levels representing anterior ...
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bioRxiv - Neuroscience 2019Quote: ... The hippocampal regions were loaded into 3 mm aluminium planchettes (Leica Cat# 1677141 for type A and 1677142 for type B ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2019Quote: ... for 3 min and uranyl acetate (Leica Ultrostain I, Leica Microsystems) for 10 min using an automatic contrasting system (Leica EM AC20 ...
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bioRxiv - Neuroscience 2023Quote: ... and sections (3 µm) were cut using a microtome (LEICA RM2255). The sections were deparaffinized by bathing twice in xylene (for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were sectioned at 3 μm using a microtome (Leica, Germany) and the sections were stained with hematoxylin–eosin (HE ...
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bioRxiv - Systems Biology 2023Quote: ... we collected 3 10 µm sections using a Microtome (Leica Byostems). We then extracted RNA using Qiagen RNAse FFPE kit according to manufacturer instructions and evaluated the percentage of total RNA fragments longer than 200 nucleotides (DV200 ...
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bioRxiv - Physiology 2019Quote: ... serial cross-sections (sections cut after on another) (7 μm) were obtained from frozen samples using a microtome (Leica Microsystems, Wetzlar, Germany). The sections were thaw-mounted on Polysine glass slides ...
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bioRxiv - Neuroscience 2020Quote: ... For immunohistochemical staining paraffin-embedded brains were cut in 7 μm thick sagittal sections with the Leica RM2155 microtome (Leica, Wetzlar, Germany)and were rehydrated in xylene and a graded alcohol series using the Leica autostainer XL (Leica ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen tissue was sectioned to 7-10 µm using a 2800 Frigocut E cryo-microtome (Reichert-Jung; Leica Instruments, Nussloch, Germany). Sections of the native tissue were fixed in 1% FA (in PBS) ...
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bioRxiv - Pathology 2021Quote: ... and then paraffin-embedded as block tissues which were subsequently sectioned with 7-μm thickness by a microtome (RM2235, Leica Microsystems Inc.). After that ...
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bioRxiv - Neuroscience 2023Quote: ... Lung samples were embedded in paraffin wax prior to sectioning at 7 µm using a Hyrax M25 Rotary Microtome (Leica Biosystem, Germany). Sections were stained with hematoxylin and ethanol-based eosin and assessed by a veterinary pathologist who was blinded to the study designs ...
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bioRxiv - Biophysics 2023Quote: ... and/or H2O2 (10 mM) were prepared immediately before measurements and condensates formation was monitored by DIC microscopy in situ (Leica LMD 7). Each sample was filled into 1.5 mm diameter quartz capillaries ...
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bioRxiv - Neuroscience 2023Quote: ... After washing in PBS (Phosphate Buffered Saline) specimens have been embedded in 7% agarose and cut into 70-100 μm sections using a vibratome (Leica VT1000S; 81). Sections have been washed three times 10 min in 0.3% PBT (PBS with 0.3% TritonX-100) ...
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bioRxiv - Plant Biology 2024Quote: ... Disease phenotypes on the coleoptiles were assessed at 7 days post inoculation by imaging lesions on a Leica M205 FA Stereomicroscope (Leica Microsystems, UK). Each experimental replicate contained 5 biological samples for each treatment (3 mock-inoculated ...
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bioRxiv - Neuroscience 2020Quote: ... 3× 1 min then washed with 70% alcohol 3x followed by Bond Wash solution (AR9590, Leica Biosystems, Wetzler, Germany) 3x ...