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Citations for Leica :
951 - 1000 of 1047 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... CldU- or IdU-labeled cells expressing Zif268 (one side) were determined using a confocal microscope with HeNe and Arg lasers (Leica, DMR TCSSP2AOBS), with a plane apochromatic 63X oil lens (numerical aperture 1.4 ...
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bioRxiv - Biophysics 2021Quote: ... The grids with live cells on them were then blotted and plunge frozen in liquid nitrogen cooled liquid ethane (Leica EM GP2) before being transferred to liquid nitrogen storage ...
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bioRxiv - Cell Biology 2021Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+ fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software (US ...
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bioRxiv - Cell Biology 2021Quote: ... HMC-1 cells were loaded into membrane carriers and were high-pressure frozen in a Leica EM PACT1 (Leica microsystems, Wetzlar, Germany). T ...
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bioRxiv - Plant Biology 2020Quote: ... The transformed protoplast cells were cultured at room temperature overnight and then observed under a Leica SP8 confocal microscope (Leica, Wetzlar, Germany).
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bioRxiv - Cancer Biology 2019Quote: ... a minimum of 5 images were taken of cells per experiment using a 20x water objective on an inverted confocal microscope (Leica TCS SP8). Using phalloidin staining and Image J software ...
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bioRxiv - Cancer Biology 2020Quote: ... Semi quantitative analysis was done by counting three independent microscopic fields (n=100-200 cells/field) for staining of specific antigen using upright microscope (Leica DM1000, Germany). Tissue array (US Biomax ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophage differentiation and L10a-EGFP expression were confirmed by observation of adherent cells and EGFP fluorescence using inverted microscope (Leica DM IRB) equipped with DC350FX camera (Leica ...
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bioRxiv - Bioengineering 2022Quote: ... The plates were then mounted into the live cell imaging chamber (Okolab, Ottaviano, Italy) attached to a Leica TCS SP8 confocal microscope (Leica, Wetzlar, Germany). The chamber was set to 5% CO2 at 37°C throughout the experiment ...
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bioRxiv - Cell Biology 2022Quote: Confocal microscopy of actin and nuclei labeled cells for actin cap validation experiments was performed under a Leica SP8 LIGHTNING (Leica Microsystems, Germany) confocal microscope with a 40x 1.2 N.A objective using 561 nm and 405 nm laser wavelengths for actin fibers and nucleus ...
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bioRxiv - Plant Biology 2021Quote: ... stained by SCRI SR2200 for cell wall staining and visualized using a confocal laser-scanning microscope (TCS SP8; Leica Microsystems, Tokyo, Japan) equipped with 592-nm STED laser ...
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bioRxiv - Cell Biology 2022Quote: The images of both GFP fluorescence and transmission of the live cells were acquired with a Leica SP8 confocal microscope (Leica, Mannheim, Germany) was performed with a 10X 0.3 NA objective ...
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bioRxiv - Microbiology 2022Quote: ... Single-cell resolution images of fixed samples were acquired using a DMI8 Leica SP-8 point scanning confocal microscope (Leica, Wetzlar, Germany) equipped with a 63x water immersion objective (Leica ...
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bioRxiv - Microbiology 2021Quote: ... Microscopic observation of cell aggregation was carried out for mating and non-mating cells using a LEICA ICC50 W microscope and the Leica Application Suite (LAS) EZ software (Leica Microsystems v3.2). Observations were performed on 10 μL of 200 μL mating and non-mating cell mixtures from a 96-well plate incubated on the bench with no shaking ...
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bioRxiv - Microbiology 2019Quote: Confocal indirect immunofluorescence microscopy imaging on fixed cells was carried out using Leica TCS SP5 Spectral Confocal Microscope (Leica Microsystems, Heidelberg, Germany) using a Leica HCX PL APO CS 63x/1.4-0.6NA objective under oil immersion ...
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bioRxiv - Cell Biology 2021Quote: ... The morphology of the spheroids was assessed and recorded using the IncuCyte S3 Live Cell Analysis System and confocal laser scanning microscope (Leica, Wetzlar, Germany) by a 5× objective using the following settings ...
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bioRxiv - Cell Biology 2020Quote: ... The grids with live cells on them were then blotted and plunge frozen in liquid nitrogen cooled liquid ethane (Leica EM GP2) before being transferred to liquid nitrogen storage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Immunology 2021Quote: ... Labelled live cells were imaged at 37 °C and 5% CO2 on a Leica Thunder Imager system (Leica Microsystems GmbH, Wetzlar, Germany), equipped with a xy motorized stage ...
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bioRxiv - Microbiology 2021Quote: ... biofilm microstructures and newly released planktonic cells were observed with a Leica SP8 confocal laser scanning microscope (CLSM; Leica Microsystem, Wetzlar, Germany) and analyzed with Imaris 7.1.0 software (Bitplane ...
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bioRxiv - Microbiology 2022Quote: ... and two confocal microscopes: a Cell Observer SD spinning disk system (Zeiss, Oberkochen, Germany) and an SP8 MP laser-scanning confocal microscope (Leica, Wetzlar, Germany). SYTO-9 showing live bacteria was excited at 488 nm wavelength using a LED (LS620 ...
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bioRxiv - Neuroscience 2022Quote: ... NeuN-positive cell staining was photographed at 5x objective on a Leica DM6 B upright microscope and DFC7000T camera (Leica Microsystems, Germany). Automated counts of the cells were obtained through ImageJ (image analysis software ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then washed a couple of times with water before they were visualized under the microscope (Leica DM IRB, TV camera). Five randomly fields were chosen to capture images using the ZEISS Efficient Navigation ...
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bioRxiv - Bioengineering 2022Quote: ... All other phase contrast and fluorescence images of micropatterned cell colonies were recorded using an inverted epifluorescence microscope (Leica DMi8; Leica Microsystems) equipped with a monochrome charge-coupled device (CCD ...
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bioRxiv - Bioengineering 2022Quote: ... The distribution of cells in the microgel matrix was assessed after 6 h using a Leica SP8 DIVE multiphoton microscope (Leica Microsystems, Germany) with 16x water immersion lens ...
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SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... Then the cells were washed in PBS and imaged in PBS-BSA using a TCS SP5 confocal microscope (Leica Microsystems, Mannheim, Germany) equipped with an HCX PL-APO 63x N.A ...
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bioRxiv - Microbiology 2022Quote: ... cells were imaged using a Stedycon optical setup (Abberior Instruments GmbH) integrated with Leica SP 5 microscope (Leica Microsystems GmbH, Manheim, Germany).
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bioRxiv - Molecular Biology 2023Quote: ... Vitreous sectioning was performed on infected cells tubes containing the vitrified sample pre-cooled at −140°C on an EM UC6/ FC7 cryo-ultramicrotome (Leica Microsystems, Vienna). The sample was trimmed to a pyramidal shaped block of 140 μm base and approximately 50 μm height using a 45° cryo trimming diamond knife (CT441 ...
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bioRxiv - Cancer Biology 2023Quote: Multiplexed immunofluorescence staining of the colon cancer TMAs was performed as previously described1 using Cell DIVE™ technology (Leica Microsystems, Issaquah, WA). After de-paraffinization and a two-step antigen retrieval ...
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bioRxiv - Neuroscience 2022Quote: ... and cell volumes (tarsi 4–5) of the mCD8-RFP-labeled neurons were analyzed by using Leica Application Suite X (Leica RRID: SCR_013673) and Imaris software (Bitplane RRID ...
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bioRxiv - Immunology 2024Quote: ... The quantifications (with a minimum of 300 cells for each condition) were performed with Leica DMI8 fluorescent microscopy (Leica Microsystems, Nanterre, France) using a 63X oil objective ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed with HBSS and live cell imaging was performed in both fluorescence and phase contrast mode using an inverted widefield microscope (Leica Thunder Imager) equipped with an environmental control box (37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: ... the 6-well plate containing the cells were mounted onto a confocal microscope (Leica TCS SP8 with a 10Å∼0.4 NA objective). They were then maintained at 37ºC (stage heater ...
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bioRxiv - Microbiology 2022Quote: ... 2-5 ml of concentrated 200 nm gold fiducials were added to the grids on the cell side before vitrification in liquid ethane using a Leica GP2 (Leica Biosystems, Germany). Fluorescence data on the vitrified grids was collected using a Linkam CMS196M cryo-correlative stage ...
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bioRxiv - Microbiology 2023Quote: ... the green fluorescence and fusion of cells transfected with pCAGGS-IBVS-EGFP plasmids were visualized under a fluorescence microscope (Leica DMi8, Germany)) ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were immediately washed in running distilled water for 1 min prior to counterstaining cell nuclei with diluted Gill’s Hematoxylin (Leica Biosystems, Wetzlar, Germany). Slides were washed under running distilled water ...
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bioRxiv - Cell Biology 2023Quote: The live gMARCO-rLC3-CHO and gMARCO-rLC3-HEK cells were observed by laser confocal microscopy (TCS-SP5, Leica Microsystems, Solms, Germany) or fluorescence microscopy (Eclipse TS100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were mounted on stubs using colloidal silver as an adhesive and sputter coated with 6 nm gold (Leica Coater ACE 200). Cells were imaged using FEI Quanta 3D scanning electron microscope operated at an accelerating voltage of 2 kV ...
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bioRxiv - Cell Biology 2023Quote: 3D live-cell imaging of mitotic epidermal root cells was performed on a Leica TCS SP8 inverted CLSM equipped with three hybrid photodetectors (Leica HyD™) and a HC PL APO 63x/1.2 W motCORR CS2 water-immersion objective ...
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Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytesbioRxiv - Cell Biology 2023Quote: ... The resin blocks were trimmed according to the marks and the trimmed resin that contained the cells of interest were cut with an ultramicrotome (Ultracut UCT; Leica, Wetzlar, Germany) at a thickness of 250 nm ...
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bioRxiv - Cell Biology 2023Quote: ... Confocal light microscopy images of cultured cells were acquired using a Leica TCS SP8 confocal laser-scanning microscope (Leica Microsystems, Wetzlar, Germany) equipped with a HC PL APO 63x/1.40 Oil objective (Leica Microsystems ...
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bioRxiv - Molecular Biology 2023Quote: ... in rich medium (YPD) were washed in synthetic medium prior to live-cell imaging with a wide-field inverted microscope (Leica DMI-6000B) equipped with Adaptive Focus Control to eliminate Z drift ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... ∼30 round chondrocytes were randomly examined in each slice preparation to select the Ca2+-fluctuation-positive cells generating spontaneous events (>0.025 in Fura-2 ratio) using commercial software (Leica Application Suite X), and recording traces from the positive cells were then analyzed using Fiji/ImageJ software for examining Ca2+ fluctuation amplitude and frequency ...
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bioRxiv - Molecular Biology 2024Quote: ... For scoring (graphs Figure 1) the percentage of cells expressing the chromatin markers were scored manually under an epi-fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
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bioRxiv - Neuroscience 2024Quote: ... Then blind codes were assigned to the specimens and immunolabeled cells were digitally captured using a fluorescence microscope (DM750, Leica Microsystems, Germany). The DCX- and Iba-1-positive cells were quantified in the hippocampal DG with the aid of the ImageJ with cell counter plugin ...
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bioRxiv - Cell Biology 2019Quote: ... Acquisition and quantification of cell migration was performed using the motorized stage of an inverted video microscope (Leica DMIRB, Leica Microsystems, Deerfield, IL), the Metamorph software and the cell counter plugin of the ImageJ software as described (Van Goethem et al. ...
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bioRxiv - Cell Biology 2020Quote: Initial microscopic screening and quantitative examination of protein sorting in cultured mammalian cell lines was performed on a confocal Leica TCS SP5 II microscope (Leica GmbH, Heidelberg, Germany). Unless stated otherwise ...
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bioRxiv - Microbiology 2019Quote: ... at pH 2.5 to quantify mucous cells in the gills per inter-lamellar unit (ILU) under a bright field light microscope (Leica DM1000, Hamburg, Germany) (29 ...
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bioRxiv - Microbiology 2019Quote: ... The cells were dehydrated stepwise from 70% to 100% ethanol before critical point drying from acetone in a CPD300 (Leica Microsystems, Vienna, Austria). After drying ...
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bioRxiv - Cell Biology 2020Quote: ... the cell culture insert was removed and the blocks of resin containing the embedded cells were trimmed and finally cut using an ultra-microtome (Leica Microsystems, Wetzlar, Germany). Ultra-thin sections with an average thickness of 55nm were transferred on formvar-coated copper grids and stained with lead citrate ...