Labshake search
Citations for Leica :
901 - 950 of 1070 citations for IL 23 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... spheroids were fixed with 4% PFA at room temperature and photographed using a Leica DM IL LED microscope (Leica Microsystems GmbH, Wetzlar, Germany). Contact inhibition ...
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bioRxiv - Cell Biology 2023Quote: ... Bright-field images were acquired using a Leica Leitz DMRXE upright microscope equipped with a DCF 300FXRI camera and Leica FireCam software (Leica Microsystems, Bannockburn, IL). Fluorescent images were acquired using a Leica SP5 confocal microscope (Leica Microsystems ...
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bioRxiv - Pathology 2023Quote: ... the vibratome stage with attached tissue was inserted into the vibratome (Model G Oxford Vibratome or Leica VT1200S, Leica Biosystems, Deerfield, IL, USA), surrounded by fresh ice ...
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bioRxiv - Microbiology 2023Quote: ... The size of the aggregates was calculated from images of live aggregated Spn captured using a Leica LMD6 with DFC3000G-1.3-megapixel monochrome camera at 40X magnification (Leica Biosystems, Buffalo Grove, IL). The top five largest aggregates were determined using ImageJ (NIH ...
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bioRxiv - Cell Biology 2023Quote: ... Acquisition and quantification of cell migration was performed using the motorized stage of an inverted video microscope (Leica DMIRB, Leica Microsystems, Deerfield, IL) and the cell counter plugin of the ImageJ software as described previously (Van Goethem et al. ...
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bioRxiv - Microbiology 2023Quote: ... were captured for each sample using a Leica LMD6 with DFC3000G-1.3-megapixel monochrome camera at 10X magnification (Leica Biosystems, Buffalo Grove, IL). Size of live bacterial aggregates was based on the top ten largest aggregates obtained using ImageJ (NIH ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were then frozen rapidly on dry ice before being coronally sectioned into 100 μm sections using a Leica CM3050 cryostat (Leica Biosystems, Richmond, IL). Slices were placed in 0.01M PBS and the ones containing the site of infection were selected to be stained ...
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bioRxiv - Neuroscience 2024Quote: ... together with Opal 520/570/690 Fluorophore Reagent pack detection (1:1000, Cat #FP1487001KT/FP1488001KT/FP1497001KT) on the Leica BOND RX Fully Automated Research Stainer (Leica, Buffalo Grove, IL) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Mouse plantaris muscles preserved for IHC were sectioned at a thickness of 7 μm using a cryotome (Leica Biosystems; Buffalo Grove, IL, USA) and adhered to multiple positively charged slides for the interrogation of various targets as explained in this and subsequent paragraphs ...
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bioRxiv - Immunology 2024Quote: ... underwent antigen retrieval at 100°C for 20 min in Epitope Retrieval solution 2 (ER2) on a Bond Max immunostainer (Leica Biosystems, Bannockburn, IL). A rat monoclonal primary antibody to mast cell protease 1 (MCPT1;Cat# 14-5503-82 ...
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bioRxiv - Cell Biology 2024Quote: ... Immunostaining images were taken using a Leica inverted microscope with a TCS SPEII confocal module and processed using LAS X software (Leica Microsystems Inc, IL).
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bioRxiv - Genetics 2019Quote: Images of human and murine tissues were analysed using the SCN400 Slide scanner software (Leica). Figures 1-4 ...
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bioRxiv - Cell Biology 2020Quote: ... neural crest populations were captured at 4 days post-fertilation (dpf) using a Leica Sp8 and analyzed with the Leica Application Suite X (Leica Microsystems, Buffalo Grove, IL) software for image capturing ...
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bioRxiv - Cell Biology 2019Quote: ... Images were acquired on a Revolution WD systems (Andor Technology Ltd., Concord, MA) mounted on a Leica DMi8 (Leica Microsystems Inc., Buffalo Grove, IL) with a 63x/1.4 NA objective lens with a 2x coupler and controlled by MetaMorph software ...
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bioRxiv - Genetics 2021Quote: ... In vivo images of retinal anatomy were acquired using a Bioptigen Envisu Preclinical Spectral Domain Optical Coherence Imaging System (Leica Microsystems, Buffalo Grove, IL). Rectangular volumes consisted of 1000 A-scans by 100 B-scans over a 2.6 x 2.6-mm area ...
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bioRxiv - Genomics 2020Quote: ... before being visualized with a Leica DM2500 microscope and photographed with a Leica DFC 7000T camera using LAS X software (Leica, Buffalo Grove, IL, USA). Goat anti-rabbit 594 (#111-515-047 ...
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bioRxiv - Neuroscience 2022Quote: ... containing the primary somatosensory cortex (S1) were cut in ice-cold (4 °C) NMDG ACSF using a Leica VT1200s vibratome (Leica Biosystems, Buffalo Grove, IL) and transferred to warm (33 °C ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were then immediately harvested and coronal brain sections (300 μm) containing the dmPFC were sliced on a Leica VT1000S vibratome (Leica Biosystems, Buffalo Grove, IL) in oxygenated (95% O2 ...
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bioRxiv - Neuroscience 2019Quote: ... transverse hippocampal slices (300 μm) were cut in ice-cold oxygenated PSS with a 1200 S Vibratome (with Vibrocheck accessory; Leica Microsystems, Bannockburn, IL, USA), and then were incubated in warm (36°C ...
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bioRxiv - Neuroscience 2019Quote: ... The brain was snap frozen using 2-methylbutane and sliced into 40-50um coronal sections using a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL). Slices were mounted on gelatin covered microscope slides and allowed to dry ...
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bioRxiv - Microbiology 2019Quote: ... and placed under a Leica TCS SP5 confocal microscope for image acquisition using a Leica 63× oil immersion objective lens (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Neuroscience 2019Quote: ... The brains were thawed to –16°C and sliced into ten series of 16 μm-thick coronal sections through the entire hypothalamus using a cryostat (CM3050 S, Leica Microsystems, Buffalo Grove, IL). Each section was thaw-mounted on Superfrost Plus slides (Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Sagittal sections (25 μm thick) were cut using a Leica CM1900 cryostat at a chamber temperature of −20 °C (Leica Biosystems, Buffalo Grove, IL) and mounted onto Superfrost-Plus Gold microscope slides (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were sectioned coronally in 30μm thick sections using a cryostat and mounted using a CryoJane tape transfer system (Leica Biosystems, Inc., Buffalo Grove, IL).
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bioRxiv - Biophysics 2020Quote: ... The image is obtained using ×25 or ×40 water immersion objective on a TCS-SP5 confocal laser scanning microscope (Leica Microsystems Inc., IL, USA).
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bioRxiv - Bioengineering 2021Quote: ... Brains were removed and horizontal cortical or hippocampal sections (300 μM thick) were cut in ice-cold ACSF using a VT1200S Vibratome (Leica, Buffalo Grove, IL, USA) and were recovered in ACSF for 30 minutes at 37 °C before treatment with MitoT-EVs.
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bioRxiv - Bioengineering 2020Quote: ... Samples were attached to SEM stubs using double-sided carbon tape and coated with Au/Pd for 30 seconds using an EM ACE200 sputtercoater (Leica, Buffalo Grove, IL, USA). Images were collected with a JSM-6010LA SEM (JEOL ...
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bioRxiv - Bioengineering 2020Quote: Networks were imaged with a DMi8 Yokogawa W1 spinning disk confocal microscope outfitted with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Z-stacks were acquired with a depth of 200 µm and step size of 5 µm ...
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bioRxiv - Biochemistry 2020Quote: ... Frozen bovine lenses were sectioned to 10 μm thickness equatorially or axially using a CM 3050 Cryostat (LEICA CM 3050S, Leica Microsystems Inc., Bannockburn, IL). Sections were thaw-mounted on Indium-Tin-Oxide (ITO ...
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bioRxiv - Neuroscience 2021Quote: ... Z stacked images were acquired using the Axio Imager (Zeiss, Germany) upright fluorescence microscope and AxioVision Imaging System (Zeiss, Germany) software or a SP8 confocal microscope (Leica Microsystems, Buffalo Grove, IL). All images were processed using Imaris (Bitplane ...
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bioRxiv - Cancer Biology 2020Quote: ... At least three randomly-selected and non-overlapping fields on each core of the tissue microarray were examined using nuclear algorithm of the Aperio ImageScope software (Leica Biosystems, Buffalo Grove, IL). Data is expressed as H-score that is based on intensity (0 ...
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bioRxiv - Bioengineering 2021Quote: ... or Toluidine Blue Stain, 1% w/v (Ricca Chemical, Arlington, TX) and scanned with a Leica Aperio ScanScope® CS2 (Leica, Buffalo Brove, IL) system ...
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bioRxiv - Microbiology 2021Quote: ... Individual images were also obtained with a 100x objective on a DeltaVision II deconvolution microscope (Applied Precision Inc., Currently Leica Microsystems, Buffalo Grove, IL). Analysis was conducted using GraphPad Prism 9 with an ordinary one-way ANOVA multiple comparisons test and unpaired t-test.
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bioRxiv - Cancer Biology 2020Quote: ... Whole mount images of primary tumors and lungs were taken with a Leica MZ10 F modular stereo microscope (Leica Microsystems, Buffalo Grove, IL, USA).
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bioRxiv - Neuroscience 2020Quote: ... OCT-embedded brains were sectioned (35 µm) throughout on a horizontal (axial) plane with a cryostat (Leica CM3050S, Leica Biosystems, Buffalo Grove, IL, USA), and collected in individual wells ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were imaged using confocal microscopy (TCS-SPE with DM6 RGBV confocal microscope; Leica DFC7000 T camera; using Leica LAS X core software; Leica Microsystems; Buffalo Grove, IL) and a 20x objective ...
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bioRxiv - Neuroscience 2022Quote: ... were collected after sectioning with a vibratome (Technical Products International; St. Louis, MO) for visualization on a confocal microscope (Leica Biosystems, Buffalo Grove, IL). Alternately ...
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bioRxiv - Neuroscience 2022Quote: ... coronal brain slices (300 μm) containing BLA were prepared in the same sucrose-based aCSF solution using a vibratome (VT1200S; Leica Microsystems, Buffalo Grove, IL). Brain slices were kept in normal aCSF (in mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Z-stack images were acquired with a step size of 0.9 µm with a Leica TCS SP5 confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Neuroscience 2022Quote: All stained sections were digitally scanned using an Aperio scanner system (Aperio AT2 - High Volume, Digital whole slide scanning scanner, Leica Biosystems, Inc., Richmond, IL) and stored in Biolucida ...
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bioRxiv - Neuroscience 2022Quote: ... while the remaining sections were selected for immunohistochemistry procedures using a Leica Bond III automated immunostainer with a diaminobenzidine chromogen detection system (DS9800, Leica Biosystems, Buffalo Grove, IL). Immunohistochemistry was performed for glial fibrillary acidic protein (GFAP ...
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bioRxiv - Microbiology 2022Quote: ... cell supernatants were collected to quantify viral particle production using a plaque assay or the cytopathic effects were evaluated using an inverted microscope and an MCI70-HD camera (Leica, Buffalo Grove, IL, USA). For the virucidal assay ...
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bioRxiv - Bioengineering 2022Quote: ... Tube formation was visualized and brightfield images were recorded using a DMi8 Yokogawa W1 spinning disk confocal microscope outfitted with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL) or Leica DMI4000 B (Leica ...
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bioRxiv - Plant Biology 2021Quote: ... The peel from abaxial side of the leaf was then placed on a microscope slide and images were collected using a DM6000B light microscope (Leica, Buffalo Grove, IL USA) This experiment was repeated 3 times to image 50 stomata from each replicated treatment and a total of 150 stomata measurements from 3 independent replicates were analyzed for each time point ...
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bioRxiv - Neuroscience 2021Quote: ... Mice brains were removed and dehydrated in 30% sucrose prior to flash-freezing with dry ice and slicing into 40 μm-thick coronal sections using a sliding microtome (Leica Biosystems, Buffalo Grove, IL). Slices were mounted and counterstained with DAPI (Vector Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... Slides were then dehydrated and cleared through graded alcohols and xylene and sealed with a coverslip and Micromount (Leica cat# 3801731, Buffalo Grove, IL) using a Leica CV5030 automatic coverslipper.
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bioRxiv - Cell Biology 2022Quote: ... Images were acquired with an ORCA-Flash 4.0 sCMOS camera (Hamamatsu Corp, Bridgewater, NJ) connected to a Leica DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL). Image analysis was performed with ImageJ.
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bioRxiv - Cell Biology 2022Quote: ... time-series image acquisition was performed with an ORCA-Flash 4.0 sCMOS camera (Hamamatsu Corp, Bridgewater, NJ) connected to a Leica DMi8 wide-field fluorescence microscope (Leica Microsystems, Inc., Buffalo Grove, IL).
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bioRxiv - Bioengineering 2020Quote: U87-MG migration across the microfluidic device was monitored daily by acquiring fluorescent images of the central gel channel with a DMi8 Yokogawa W1 spinning disk confocal microscope with a Hamamatsu EM-CCD digital camera (Leica Microsystems, Buffalo Grove, IL). Three regions of interest (ROI ...
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bioRxiv - Cell Biology 2019Quote: ... Sample block was then re-mounted using Durcupan on a copper stud (Xu et al., 2017) and trimmed using ultramicrotome (EM UC7, Leica Microsystems, Buffalo Grove, IL). Finally ...