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Citations for Leica :
801 - 850 of 1539 citations for IL 10 Rat since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... and imaged on a Leica SP8 STED 3X/Confocal Microscope using 100×/1.4 oil immersion objective lens (Leica Microsystems, Buffalo Grove, IL). All other imaging was performed on a Zeiss 780 LSM confocal microscope using 63×/1.4 oil immersion objective lens.
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bioRxiv - Biochemistry 2021Quote: ... then dehydrated and cleared through graded alcohols and xylene and sealed with a coverslip and Micromount (Leica cat# 3801731, Buffalo Grove, IL) using a Leica CV5030 automatic coverslipper.
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bioRxiv - Cell Biology 2021Quote: ... The cells were observed using an objective lens (N Plan 5x/0.12 PH 0) on a Leica DM IL LED fluorescence microscope (Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Physiology 2021Quote: ... paraffin sections were imaged using a Leica SP8 confocal/multiphoton microscope and Coherent Chameleon Vision II Ti:Sapphire laser (Leica, Buffalo Grove, IL) tuned to a wavelength of 910 nm.
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bioRxiv - Cancer Biology 2021Quote: ... Optical sections were acquired through sequential scans or brightfield/differential interference contrast on a TCS SP5 confocal microscope (Leica Microsystems, Deerfield, IL) using a ∼40 Å (numerical aperture ...
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bioRxiv - Immunology 2021Quote: ... Images were taken using a Leica DM6000B fluorescent microscope, Orca Flash 4.0 mounted digital camera (Hamamatsu Photonics K.K., Japan) and LAS Advanced Fluorescence software (Leica Microsystems, Buffalo Grove, IL). Fluorescent channels were photographed separately and then merged ...
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bioRxiv - Microbiology 2020Quote: ... All images were captured with a Leica DM3000 microscope and DFC 500 digital camera using Leica Application Suite version 4.10.0 (Leica Microsystems, Buffalo Grove, IL).
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bioRxiv - Immunology 2020Quote: ... At least 150 cells per mouse were examined and quantified for cell type following the manufacturer’s instructions using a CME microscope (Leica, Buffalo Grove, IL) with a 40x objective ...
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bioRxiv - Cell Biology 2021Quote: Confocal images were acquired on a TCS SP8 laser scanning confocal microscope (LSCM) equipped with a 63×/1.4 numerical aperture (NA) oil objective (Leica, Buffalo Grove, IL). Imaging processing and quantitative image analysis of Duolink signal was done using Cell Profiler (MIT ...
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bioRxiv - Bioengineering 2021Quote: ... The cell cultures were followed daily for up to a week using light microscopy (Leica DM IL LED; Leica Microsystems, Wetzlar, Germany).
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bioRxiv - Developmental Biology 2022Quote: ... abdomens were dissected in RNAlater-ICE using a Leica M80 stereomicroscope with an IC80HD camera attached (Leica Microsystems, Buffalo Grove, IL, USA). While dissecting ...
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bioRxiv - Plant Biology 2022Quote: ... Fluorescence signals were examined 48-72 hours after agroinfiltration using a Leica SP8 Inverted Confocal Microscope (Leica Microsystems Inc., Buffalo Grove, IL). Excitation and emission wavelengths for mVenus were 488 nm and 500-550 nm ...
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bioRxiv - Neuroscience 2023Quote: ... brains were perfused as described above and were coronally sectioned (2 mm thick) using a vibratome (Leica Biosystems, Buffalo Grove, IL, USA). The brain slices were cleared using the Passive Clarity protocol (Yang et al. ...
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bioRxiv - Pathology 2022Quote: Immunohistochemistry (IHC) was performed on kidney sections using an automated stainer and system reagents (Leica Bond RX, Leica Biosystems, Buffalo Grove, IL). IHC stains were selected based on their routine use in published studies of the adenine diet model and included the F4/80 stain for murine macrophages and the KIM-1 and NGAL stain for renal tubular injury9 ...
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bioRxiv - Immunology 2022Quote: ... Tissue samples were then cut into 12-micron sections for immunofluorescence staining using a Leica CM1520 cryostat (Leica Biosystems, Deer Park, IL). Samples were first fixed in methanol in −20°C then treated with Antigen Retrieval Buffer (pH 6 ...
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bioRxiv - Immunology 2023Quote: ... The coverslips were then mounted on glass slides and imaged using a confocal microscope (LEICA TCS SP8, Leica Microsystems, Buffalo Grove, IL) with a 40 X oil objective.
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bioRxiv - Neuroscience 2023Quote: Each NDG was sectioned at 16 μm into serial sections using a Leica CM3050 S cryostat (Leica, Buffalo Grove, IL, United States). Sections were immediately mounted and slides were dipped in ethanol (100% ...
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bioRxiv - Neuroscience 2023Quote: ... A single 250 μm-thick parasagittal slice was cut for each mouse in this ice-cold solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1000S; Leica, Deerfield, IL). Slices were then recovered at 32°C for 20-30 minutes in oxygenated artificial cerebrospinal fluid (ACSF ...
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bioRxiv - Genomics 2022Quote: ... The crickets where then observed under a fluorescent dissecting microscope with digital camera attached (Leica M125, Leica Microsystems Inc., Deerfield, IL, USA) for either different eye coloration from wild type in knock-out treatments (using a standard white LED ring light ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were baked at 60 °C for 15 min and deparaffinized with the Leica Bond Dewax solution (Leica Biosystems, Deer Park, IL), followed by heat-based antigen retrieval using Bond Epitope Retrieval Solution 1 (Leica Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... then sectioned into 250 μm-thick slices in the oxygenated ice-cold cutting solution using a sapphire blade (Delaware Diamond Knives, Wilmington, DE) on a vibratome (VT1200S; Leica, Deerfield, IL). The slices collected were allowed to recover at 30°C for 20 minutes in oxygenated saline solution (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was removed from the skull and sliced at 250 μm using a vibrating microtome (VT1000s, Leica Biosystems, Buffalo Grove, IL) in cold ...
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bioRxiv - Cell Biology 2023Quote: ... USA) and Bioptigen Envisu R2200 ultra-high resolution (UHR) spectral-domain optical coherence tomography system (SD-OCT; Leica Microsystems, Deerfield, IL, USA) (22) ...
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bioRxiv - Neuroscience 2024Quote: ... Selected sections were also studied on a Leica SP5 Tandem Scanner Spectral 2-photon confocal microscope (Leica Microsystems, Inc., Buffalo Grove, IL) or scanned with an Olympus VS200 Research Slide Scanner (Olympus / Evident ...
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bioRxiv - Immunology 2024Quote: ... and tonsil sections of 4 µm-thick were deparaffinized at 70°C in 100% Clearene solvent (Leica Biosystems, Buffalo Grove, IL, USA) solution and washed in ethanol (EtOH ...
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bioRxiv - Neuroscience 2024Quote: Hemibrains were drop-fixed in 4% paraformaldehyde for two days and sectioned at 40 mm on a vibratome (Leica, Buffalo Grove, IL). Sections were collected in a 1 in 4 series and stored in PBS at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The images were acquired using inverted microscope with 10 × 10 magnification (Leica DMI 6000B, Leica, Germany). Image J software (NIH ...
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bioRxiv - Cell Biology 2020Quote: ... The images were acquired using inverted microscope with 10 × 10 magnification (Leica DMI 6000B, Leica, Germany). Image J software (NIH ...
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bioRxiv - Cell Biology 2021Quote: ... Each field was obtained at a magnification of 10×10 (microscope Leica and digital camera Sigeta). Collagen area and total field area were determined using the ImageJ software (version 1.52a) ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal slices (300 and 350 µm for mice and rats, respectively) containing the NAc were prepared on a VT1200S vibratome (Leica, Germany) in 4°C cutting solution containing (in mM) ...
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bioRxiv - Neuroscience 2022Quote: ... and Homo (N=5 males) Gcg-Cre rats from the Janvier colony were cut into 50 μm coronal slices using a cryostat (Leica CM3050S). The cNTS was then microdissected using biopsy punches (Integra Miltex ...
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bioRxiv - Bioengineering 2023Quote: ... The images were collected from at least three sections of nerve tissues for each rat under a Leica DMi8 fluorescent microscope (Leica Microsystems). Quantitative analysis of images was performed using Image J (v1.8.0 ...
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bioRxiv - Microbiology 2021Quote: ... fixed with 10% Neutralized formalin (Leica), blocked ...
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bioRxiv - Cancer Biology 2021Quote: ... was done on sections of FFPE tissue using a Bond III automated immunostainer and the Bond Polymer Refine detection system (Leica Microsystems, IL, USA), with the following antibodies and conditions (dilution ...
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bioRxiv - Cell Biology 2019Quote: ... Acquisition and quantification of cell migration was performed using the motorized stage of an inverted video microscope (Leica DMIRB, Leica Microsystems, Deerfield, IL), the Metamorph software and the cell counter plugin of the ImageJ software as described (Van Goethem et al. ...
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bioRxiv - Cell Biology 2019Quote: Microscope images of fixed Drosophila follicles were obtained using LAS AF SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20×/0.60 IMM CORR -/D (Leica Microsystems, Buffalo Grove, IL), Zen software on a Zeiss 880 mounted on Zeiss Axio Observer.Z1 using Plan-Apochromat 20x/0.8 working distance (WD ...
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bioRxiv - Immunology 2022Quote: ... was performed on sections (5 mm thick) of formalin-fixed lung mounted onto charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems) ...
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bioRxiv - Molecular Biology 2020Quote: ... The nuclei were imaged using a Leica DM6 B microscope equipped with Leica DMC5400 camera or DM IL LED fluorescence microscope equipped with Leica DFC450 C (Leica Microsystems, Wetzlar, Germany) camera and the images were processed using ImageJ software version 1.53 (National Institutes of Health ...
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bioRxiv - Bioengineering 2022Quote: ... and stained using immunohistochemistry (IHC) for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Cell Biology 2022Quote: ... cryosectioned parallel (axially) to the optic axis at 20 μm thickness using a Leica CM3050 S cryostat (Leica Biosystems Inc; Buffalo Grove, IL), and finally were transferred onto plain microscope slides ...
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bioRxiv - Immunology 2022Quote: ... and stained using immunohistochemistry for collagen I (1:4000, polyclonal rabbit, lifespan biosciences, Seattle WA) via a Bond-Max autostaining system (Leica biosystems, Lincolnshire, IL). Stained kidneys were scanned at high resolution using a CRi Panoramic SCAN 40x Whole Slide Scanner (Perkin-Elmer).
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bioRxiv - Immunology 2021Quote: ... The organs were then embedded into paraffin wax using a HistoCore Arcadia C and H paraffin embedding station (Leica Biosystems, Buffalo Grove, IL). Each organ was sliced into 4 µm sheets using a RM2235 manual microtome (Leica Biosystems ...
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bioRxiv - Immunology 2021Quote: ... The fixed organs were moved to a 70% ethanol solution and processed with an ASP300 S tissue processor (Leica Biosystems, Buffalo Grove, IL) for dehydration into paraffin ...
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bioRxiv - Physiology 2019Quote: Fixed whole EHBDs were imaged using a Leica SP8 confocal/multiphoton microscope and Coherent Chameleon Vision II Ti:Sapphire laser (Leica, Buffalo Grove, IL, USA) tuned to a wavelength of 910 nm ...
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Fascin regulates protrusions and delamination to mediate invasive, collective cell migration in vivobioRxiv - Cell Biology 2019Quote: Microscope images of fixed Drosophila follicles were obtained using LAS AS SPE Core software on a Leica TCS SPE mounted on a Leica DM2500 using an ACS APO 20x/0.60 IMM CORR -/D objective (Leica Microsystems, Buffalo Grove, IL) or using Zen software on a Zeiss 700 LSM mounted on an Axio Observer.Z1 using a Plan-Apochromat 20x/0.8 working distance (WD ...
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bioRxiv - Cancer Biology 2020Quote: ... stain quantification of total tumor area (necrotic and stromal areas removed) and percent positive stain or stain intensity was performed using ImageScope Aperio Analysis software (Leica, Buffalo Grove, IL). Areas for quantification were annotated using Aperio analysis tools and percent weak ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were then rinsed briefly with PBS and then sectioned at a thickness of 100 μm on a vibratome (VT1200 Leica Biosystems, IL, USA). Brain sections were incubated in a blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... Each slide was analyzed by an investigator blinded to the identity of the experimental material using a Leica SP8 upright confocal microscopy (Leica Microsystems, IL, USA) and ImageJ and Plugin Cell Counter (NIH Image ...
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Sterol flow between the plasma membrane and the endosome is regulated by the LAM family protein Ltc1bioRxiv - Cell Biology 2019Quote: Filipin and Calcofluor imaging (Fig1G, 2D, 6B) was done by wide field microscopy acquired with the Leica AS AF software (Leica MicroSystems, Bannockburn, IL).
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bioRxiv - Plant Biology 2020Quote: ... Thick sections (1 µm) of long shoots were cut with glass knives using an Ultracut R microtome (Leica Microsystems, Buffalo Grove, IL, USA) and stained with toluidine blue ...