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1 - 12 of 12 citations for Zika Virus Lysate since 2019

• HIV-1 accessory protein Vpr interacts with REAF/RPRD2 to mitigate its antiviral activity

  Joseph M Gibbons, et al., bioRxiv - Microbiology 2019

  Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
• The TRIP6/LATS1 complex constitutes the tension sensor of α-catenin/vinculin at both bicellular and tricellular junctions

  Lin Xie, et al., bioRxiv - Cell Biology 2023

  Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
• Adrenomedullin promotes interneuron migration in a dual human model for hypoxic interneuronopathy of prematurity

  Wojciech P. Michno, et al., bioRxiv - Neuroscience 2023

  Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
• Neural G0: a quiescent-like state found in neuroepithelial-derived cells and glioma

  Heather M. Feldman, et al., bioRxiv - Cell Biology 2020

  Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
• Keap1 recognizes EIAV early accessory protein Rev to promote antiviral defense

  Yan Wang, et al., bioRxiv - Microbiology 2021

  Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
• Th17 T cells and immature dendritic cells are the preferential initial targets after rectal challenge with an SIV-based replication-defective dual-reporter vector

  Danijela Maric, et al., bioRxiv - Immunology 2021

  Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
• ANP32A and ANP32B are key factors in the Rev dependent CRM1 pathway for nuclear export of HIV-1 unspliced mRNA

  Yujie Wang, et al., bioRxiv - Microbiology 2019

  Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
• Simultaneous in vivo Pharmacokinetics and Pharmacodynamics of TDF Intravaginal Ring during High-dose Vaginal SHIV Challenge in Pigtail Macaques

  Katarina Kotnik Halavaty, et al., bioRxiv - Pharmacology and Toxicology 2021

  Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
• Extracellular Domains of CAR Reprogram T-Cell Metabolism Without Antigen Stimulation

  Aliya Lakhani, et al., bioRxiv - Systems Biology 2023

  Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
• Contribution of 6mer seed toxicity to HIV-1 induced cytopathicity

  Aparajitha Vaidyanathan, et al., bioRxiv - Microbiology 2022

  Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
• Emergence of compensatory mutations reveal the importance of electrostatic interactions between HIV-1 integrase and genomic RNA

  Christian Shema Mugisha, et al., bioRxiv - Microbiology 2022

  Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
• Functional Implications of the Exon 9 Splice Insert in GluK1 Kainate Receptors

  Surbhi Dhingra, et al., bioRxiv - Neuroscience 2023

  Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...