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Citations for Polysciences :
1 - 50 of 104 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... Yeast cells were then incubated with 0.01% Uvitex2B (a chitin marker; Polysciences) and mAb 18B7 (10 µg/ml ...
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bioRxiv - Immunology 2022Quote: ... Expression and production of nanobodies were conducted by transfecting the expression vectors into the HEK293F cells using polyethyleneimine (PEI) (Polysciences). After approximately 96 h ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids and PEI (product #260008-5; Polysciences) were separately diluted in Opti-MEM1 (product #31985-047 ...
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bioRxiv - Biochemistry 2021Quote: ... Expression plasmids (Nsp1/Nsp2/N) and PEI (Polysciences, 260008-5) were separately diluted in Opti-MEM1 (Gibco 31985-047 ...
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bioRxiv - Neuroscience 2023Quote: ... Expression plasmids were transfected using polyethylenimine (PEI) (23966-1, Polysciences). 10 ug and 200 ng of total plasmid was used for 100 mm cell culture dishes and 8-well plates respectively with a Plasmid:PEI ratio of 1:4 ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected with a plasmid harboring an import signal of yeast mitochondrial outer membrane Tom70p fused to EGFP21 using pEGFP-N1 as a backbone (Mito-EGFP) using PEI Max (Polysciences) and cultured for 2 days ...
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bioRxiv - Genetics 2021Quote: ... expression plasmids into HEK293T cells using a 1mg/mL PEI solution (Polysciences). We collected lentiviral supernatants at 48 hours and 72 hours after transfection and concentrated lentiviruses by ultracentrifugation for 120 minutes at 19,500 rpm using a Beckman SW28 ultracentrifuge rotor at 4°C.
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bioRxiv - Biochemistry 2022Quote: ... The expression vector was transfected into HEK293T cells using PEI’MAX’ (Polysciences Inc.). Cells were cultured for 40 h post-transfection and detached from the culture vessels using trypsin/EDTA for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were transiently transfected with the expression plasmid and polyethyleneimine (PEI) (Polysciences) at a density of 2.0-2.5×106 cells per ml ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transiently transfected with the expression plasmids using polyethylenimines (PEI, Polysciences). In details ...
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bioRxiv - Cell Biology 2021Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (19 ...
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bioRxiv - Biophysics 2021Quote: ... with pre- incubated expression vector (300 µg/L) and polyethyleneimine (PEI) MAX (Polysciences) (900 µg/L) ...
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bioRxiv - Cell Biology 2019Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) essentially as described previously (19,37) ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were transiently transfected with the expression plasmids and polyethylenimines (PEIs) (Polysciences). For expressions of the RS head component ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection of cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.) as described previously (29 ...
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bioRxiv - Cell Biology 2021Quote: Transfection of Capsaspora cells with expression plasmids was performed using polyethylenimine (PEI) (Polysciences, Inc.). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... adherent HEK293T cells were transfected with the expression plasmids using linear PEI (MW 25000, Polysciences). In detail ...
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bioRxiv - Cancer Biology 2022Quote: ... and 7.5 µg of DNA expression vector) with 30 µg polyethylenimine (PEI, Polysciences, 23966, USA), and concentrated with Lenti-X concentrator (CloneTech ...
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bioRxiv - Microbiology 2023Quote: ... or an MLV Gag-Pol expression plasmid and an LHCX-derived vector using polyethyleneimine (PolySciences). Expression was induced in pLKO transduced cell lines through an overnight treatment with 500ng/ml doxycycline hyclate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... MRC-5 cells were transfected with the SOD2-V5 expression construct using Polyethylenimine “MAX” (Polysciences Inc.), cultured for 16 h ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Biochemistry 2020Quote: ... The expression construct was transiently transfected in HEK 293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Each expression vector was transiently transfected into Expi293 cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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bioRxiv - Biochemistry 2021Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... with equal amounts of Ig heavy and light chain expression vectors using polyethylenimine Max (PEI- MAX, Polysciences) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Microbiology 2024Quote: ... expression medium was added to reduce the cell density to 1.0 × 106/ml for polyethyleneimine (PEI; Polysciences) transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... The cells were transiently transfected with ADAMTS6 WT or ADAMTS6 EA expression plasmids using PEI MAX (24765; Polysciences) and were co-cultured with Fbn1-/- mouse embryo fibroblasts (MEFs ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected with 1 μg/mL of each expression vector using 2 μg/mL Polyethyleneimine “MAX” (PolySciences) (Hirayama et al. ...
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bioRxiv - Microbiology 2020Quote: ... Then cells were transiently transfected with 2mg expression plasmid per 1 liter using the Polyethylenimine (PEI) MAX (Polysciences) at a density of 1.0 x 106 cells/ml in fresh Union 293 medium ...
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bioRxiv - Bioengineering 2022Quote: ... 750 ng of ABEs and 250 ng of sgRNA expression plasmids were co-transfected using polyethyleneimine (PEI, Polysciences) following the manufacturer’s recommended protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Human embryonic kidney 293A cells were then used to produce full length whole Ab 1116NS19.9 or Ab 5b1 from their respective p3BNC expression plasmids that were transfected with polyethylenimine reagent (PEI; Polysciences). Functionality of cloned secreted 1116NS19.9-hIgG1 and 5b1-hIgG1 antibodies was tested by ELISA against antigen-coated plates.
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Biochemistry 2020Quote: ... All protein encoding sequences were subcloned to pCAG vector with N-terminal FLAG-tag for expression in HEK293F cell lines transfected by polyethylenimines (PEIs, Polysciences). Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc. ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Microbiology 2019Quote: ... expression medium was added to reduce cell density to 1.0×106 ml-1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Microbiology 2020Quote: ... at a density of 2.5 × 106 cells/ml were transfected with microbody expression vector plasmid DNA using polyethyleneimine (Polysciences, Inc) at a 1:3 plasmid:PEI ratio ...
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bioRxiv - Bioengineering 2023Quote: ... The light and heavy chain expression plasmids of LH03 or LH05 were mixed with 25 kDa linear polyethylenimine (PEI, Polysciences) and transiently transfected in HEK293E cells ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Developmental Biology 2024Quote: ... The lentivirus was produced by co-transfection of the expression vector coding Lifeact-mCherry and lentiviral packaging plasmids (pCMVdR8.91 and pMD2.G) using PEI MAX (Polysciences #24765) into HEK293T cells ...
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bioRxiv - Microbiology 2020Quote: ... and MERS CoV lacking the endoplasmic retention signal sequence were cloned into pCAGGS expression system (pCAGGS-SARS2-S, pCAGGS-SARS-S, and pCAGGS-MERS-S) and were transfected using Polyethylenimine (PEI) (Polysciences, USA) at 1:3 ratio in HEK293T cells ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with IRE1 expression plasmids or pcDNA4 as control (500 ng per well) using PEI (linear 25 kDa, Polysciences, Inc.) at a DNA:PEI mass ratio of 1:3 ...