Labshake search
Citations for Polysciences :
1 - 50 of 53 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2019Quote: ... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... with 2.25 μg Polyethylenimine in water (PEI, pH 7.3, 23966, Polysciences) and 250 ng plasmid DNA ...
-
bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
-
bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
-
bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
-
bioRxiv - Neuroscience 2020Quote: ... we used FastBlue (5% in distilled water, 17740-1, Polysciences, Inc, Warrington, PA), fluorescent latex microspheres (red retrobeads IX or green retrobeads IX ...
-
bioRxiv - Microbiology 2021Quote: ... aerosols were generated from ultrapure water containing polystyrene and silica nanoparticles (00876-15, Polysciences; SISN100-25M ...
-
bioRxiv - Biophysics 2021Quote: ... The water surrounding the spheres was seeded with 2 µm polystyrene spheres (Polysciences 19814). PIV analysis was performed using PIVlab [53] ...
-
bioRxiv - Cancer Biology 2022Quote: ... and fixed with 2% methanol-free formaldehyde (Polysciences, Warrington, PA). Cells were then permeabilized with 0.1% saponin (Beckman Coulter ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
-
bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
-
bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... Parasites were cross-linked in 1% methanol-free formaldehyde (Polysciences Inc) at 37°C for 10 min while kept in suspension on an orbital shaker (50 rpm) ...
-
bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in 4% methanol free PFA (Polysciences Cat# 040181) for 3 hrs at room temperature (RT ...
-
bioRxiv - Biophysics 2021Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
-
bioRxiv - Biophysics 2020Quote: ... then in sterile water and mounted with a Mowiol® 4-88 mounting medium (Polysciences, Inc.). Montage was allowed to solidify in the dark for 48h before microscope acquisitions.
-
bioRxiv - Immunology 2022Quote: ... Cells were then washed and fixed with 4% formaldehyde (methanol-free, Polysciences). In this assay ...
-
bioRxiv - Developmental Biology 2023Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
-
bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
-
bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were fixed using an osmotically balanced and methanol-free 4 % formaldehyde (Polysciences) solution which has previously been shown to not cause detectable nuclear shrinkage (59) ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 5 µg of transfer plasmid were transfected with polyethylenimine (8mg/mL sterlile water, Polysciences, Warrington, PA, USA). After 60–72 h of incubation ...
-
bioRxiv - Neuroscience 2024Quote: ... The reaction was blocked by washing the sections with distilled water and mounted with Poly-Mount (#08381, Polysciences). Brightfield images were acquired with Nikon E800 microscope ...
-
bioRxiv - Neuroscience 2020Quote: ... Monolayer cells grown on glass coverslips were fixed in 4% methanol-free formaldehyde (Polysciences), diluted in DPBS (ThermoFisher Scientific ...
-
Fast and sensitive diffuse correlation spectroscopy with highly parallelized single photon detectionbioRxiv - Neuroscience 2020Quote: Our decorrelating tissue phantom was created by a 1 μm polystyrene microsphere-in-water suspension (Polysciences, inc.; Warrington, PA) in custom made cuvettes ...
-
bioRxiv - Neuroscience 2023Quote: ... the coverslips were rewashed with 1x PBS and milli-Q water and finally mounted with AquaMount (Polysciences, 18606-20). To assess cell death ...
-
bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for five minutes and then stained in Weigert’s Iron Hematoxylin working solution (Polysciences) for five seconds ...
-
bioRxiv - Biophysics 2023Quote: ... For this study 1:100 dilution of bead solution in double distilled water (Flurospheres amine-modified, 0.2 μm, yellow-green fluorescent, Microspheres, Polysciences) was used ...
-
bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for two minutes and then stained in Beibrich Scarlett-Acid Fuchsin Solution (Polysciences) for 15 seconds ...
-
bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
-
bioRxiv - Developmental Biology 2022Quote: ... The free nuclei were then bound to 300 μL of activated concanavalin A beads (Polysciences #86057) at RT for 10mins ...
-
bioRxiv - Biochemistry 2020Quote: ... washed in two drops of water and stained 30 s in either 0.75% uranyl formate (UF) or 2% sodium phosphotungstate (NaPT, Polysciences Inc. USA), pH 7.5.
-
bioRxiv - Bioengineering 2021Quote: ... DexMA was dissolved at 0.5 g mL-1 in a 1:1 mixture of milli-Q water and dimethylformamide with 1% (w/v) Irgacure 2959 photocrosslinker and 0.625 mM methacrylated rhodamine (Polysciences, Inc., Warrington, PA). For matrix displacement studies ...
-
bioRxiv - Neuroscience 2019Quote: ... slides were dipped in distilled water and Menzel-Gläser #1,5 coverslips were mounted using Aqua-Poly/Mount mounting medium (Polysciences, Inc., Cat# 18606-20).
-
bioRxiv - Biophysics 2022Quote: ... 100-200 µg of purified bacmid in 1 mL water were incubated with 400 µg of 25000 MW polyethyleneimine (PEI; Polysciences Cat# 23966) in 1 mL water at 55 degC for 30-45 minutes to sterilize ...
-
bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free formaldehyde (FA) (Polysciences, 040181) during 2 hours at RT and then rinsed in PBSDT (PBS 1x ...
-
bioRxiv - Cell Biology 2020Quote: ... For acquisition of time-lapse images young adult animals were immobilized with 0.05 micron polystene polybeads diluted 1:2 in water (Polysciences Inc., Warrington, PA, USA) and mounted on slides with 5% agarose pads ...
-
bioRxiv - Genomics 2022Quote: ... aligned nanofibrous dual-component scaffolds were developed with approximately a 50:50 ratio of PCL and water-soluble polyethylene oxide (PEO; 200 kDa, Polysciences, Inc., Warrington, PA). Briefly ...
-
bioRxiv - Bioengineering 2019Quote: Neurons were fixed in methanol-free 4% formaldehyde ultra-pure EM grade (Polysciences, Inc., cat. no. 18814-10) + 4% sucrose in PBS for 20 min at room temperature and permeabilized with 0.2% Triton X-100 for 5-10 min at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
-
bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
-
bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
-
bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
-
bioRxiv - Immunology 2020Quote: ... pPPI4 DNA vectors carrying free SOSIP trimers or SOSIP-based antigen-bearing components were transfected into FreeStyle 293F cells using polyethyleneimine (Polysciences, Inc) as described previously(6) ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed and incubated in serum-free MEM media for 2 h prior to addition of 1.75-micron latex beads (Fluoresbrite Carboxylate YG Microspheres, Polysciences, Inc, PA). Cells were incubated with beads at 37°C for an additional 2 h ...
-
bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
-
bioRxiv - Cell Biology 2019Quote: The tail fin was amputated up to the level of the notochord and larvae were fixed 20-24 hours later in 1ml of 4% ethanol-free formaldehyde (Polysciences, Warrington, PA) in phosphate-buffered saline (PBS ...
-
bioRxiv - Neuroscience 2023Quote: ... 2 x 106 HEK293 cells were seeded onto 100 mm2 cell culture dishes and transfected 24 hours later with a 12 μg cDNA mix prepared in Opti-MEM serum-free media with the polyethylenimine (PEI) (Polysciences #23966-2) transfection agent at a 3:1 ratio (PEI ...
-
bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...