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Citations for Polysciences :
1 - 50 of 359 citations for Oxychlordane Unlabeled 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... 60 μl of 1 ug/ml PEI MAX (Polysciences Inc.). After 16 hours ...
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bioRxiv - Developmental Biology 2021Quote: For sectioning embryos in 100% methanol were mounted in JB4 plastic sectioning medium (Polysciences cat. no 00226-1). Dehydrated samples were infiltrated with 10 ml JB4 solution A plus catalyst for two days ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Cancer Biology 2019Quote: ... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ...
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bioRxiv - Systems Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-domain library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Synthetic Biology 2022Quote: ... pMDLg/pRRE) and 8 ug of rTetR-concatenation library vectors using 50 mL of polyethylenimine (PEI, Polysciences #23966). Packaging plasmids were gifts from Didier Trono ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
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bioRxiv - Synthetic Biology 2024Quote: ... one day after plating 2ug of each plasmid (maximum 8 ug of plasmid) was transfected into cells using 60 ug of PEI max (Polysciences). Producer cells were then maintained for 3 days after transfection ...
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bioRxiv - Immunology 2023Quote: ... linear polyethylenimine (PEI, 25 kDa, PolySciences, 30 ug) suspended in Opti-MEM (500 uL ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Biophysics 2021Quote: ... coated with polyethlenimine (100 µg/ml; Polysciences). Neurons were incubated in 5% CO2 at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and fixed with 2% methanol-free formaldehyde (Polysciences, Warrington, PA). Cells were then permeabilized with 0.1% saponin (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Neuroscience 2020Quote: ... brain organoids were placed directly in 4% methanol-free formaldehyde (Polysciences) diluted in DPBS (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Parasites were cross-linked in 1% methanol-free formaldehyde (Polysciences Inc) at 37°C for 10 min while kept in suspension on an orbital shaker (50 rpm) ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were fixed in 4% methanol free PFA (Polysciences Cat# 040181) for 3 hrs at room temperature (RT ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ug of the MAXI-prep DNA that was pre-diluted in 13 ml of Opti-MEM® I Reduced Serum Medium (Thermo, 31985-062) and 800 ug Polyethylenimine (PEI 25K, Polysciences CatNo 23966-1) likewise pre-diluted in 13 ml of Opti-MEM media ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed and fixed with 4% formaldehyde (methanol-free, Polysciences). In this assay ...
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bioRxiv - Developmental Biology 2023Quote: ... Dechorionated embryos were fixed in 10% formaldehyde (methanol free, 18814 Polysciences Inc.) in PBS:Heptane (1:1 ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed using an osmotically balanced and methanol-free 4 % formaldehyde (Polysciences) solution which has previously been shown to not cause detectable nuclear shrinkage (59) ...
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bioRxiv - Neuroscience 2020Quote: ... Monolayer cells grown on glass coverslips were fixed in 4% methanol-free formaldehyde (Polysciences), diluted in DPBS (ThermoFisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 18 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 1,000 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 2.93 µL of 1 mg/mL PEI MAX (Polysciences #24765-100) were dissolved in 300 µL of 1x PBS and applied to the cell culture ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were dehydrated in series of methanol concentrations and embedded in methacrylate resin (JB4, Polysciences) containing eosin and acridine orange as contrast agents (Weninger et al. ...
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bioRxiv - Cell Biology 2023Quote: ... or 12mm circular #1.5 coverslips (Neuvitro) coated with polyethylenimine (100 µg/ml; Polysciences). Neurons were incubated in 5% CO2 at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free FA (Polysciences, 040181) overnight at 4°C and stored in 100% methanol at -20°C ...
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bioRxiv - Developmental Biology 2021Quote: Dechorionated and anesthetized embryos were fixed at desired stages in 4% methanol-free formaldehyde (FA) (Polysciences, 040181) during 2 hours at RT and then rinsed in PBSDT (PBS 1x ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were incubated in 50:50 mixture of methanol and JB4 resin (Polysciences, 00226-1, GMBH, Germany) overnight ...
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bioRxiv - Bioengineering 2019Quote: Neurons were fixed in methanol-free 4% formaldehyde ultra-pure EM grade (Polysciences, Inc., cat. no. 18814-10) + 4% sucrose in PBS for 20 min at room temperature and permeabilized with 0.2% Triton X-100 for 5-10 min at room temperature ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... and LPS (100 μg/ml) coupled to 3-μm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Cell Biology 2023Quote: Three biological replicates of NF54 and NF54CSAh (at 20±2 h.pi.) were fixed with 1.25 % methanol-free formaldehyde (Polysciences Inc) for 25 min at 37°C and quenched with 150 mM glycine for 15 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... and LPS (100 µg/mL) covalently coupled to 3-µm amino polystyrene beads (Polysciences Inc., Warrington, PA) as described previously (Mantegazza & Marks ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μg of plasmid DNA was incubated with 100 μg/mL polyethyleneimine (Polysciences Inc; linear 25 kDa) in 500 μL DMEM without serum or antibiotic for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Cell Biology 2022Quote: ... After that, pellets were dehydrated through a series of methanol (50%, 70%, 90%) then infiltrated with LR Gold resin (Polysciences) according to the manufacturer’s instructions and allowed to polymerize at -10°C under an UV lamp for 24 h ...
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bioRxiv - Biochemistry 2023Quote: ... HEK293T were seeded in 10-cm dishes then transfected with 10 ug of myc-tagged hnRNPA1 and hnRNP A1dm mutant using polyethyleneimine (PolySciences). Cells were treated with 100 uM 4-SU 16 hours prior to UV-crosslinking ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviruses were produced by transfection of HEK 293T cells with VSV.G and gag-pol plasmids together with the plasmid of interest and PEI (2ug per ug of DNA, Polysciences Inc) in Opti-MEM medium (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2021Quote: ... and HAP1 cells were initially tested for absence of mycoplasma contamination (UC Berkeley Cell Culture facility) by fluorescence microscopy of methanol fixed and Hoechst 33258 (Polysciences, #09460) stained samples ...
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bioRxiv - Cell Biology 2021Quote: Samples were crosslinked for 10 min at RT and 20 min at 4°C with 0.8% formaldehyde (methanol free, ultrapure EM grade, Polysciences, Inc; Warrington PA). Reaction was stop at RT for 10 min with the addition of Glycine to a final concentration of 0.125 M ...
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bioRxiv - Cell Biology 2024Quote: Midguts were dissected in 1X PBS (Oxoid, BR0014G) and fixed for 20 minutes at room temperature in fresh 4% methanol-free paraformaldehyde (Polysciences, 18814-10) diluted in PBS ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Genomics 2023Quote: ... Expi293F cells at a density of 3×106 cells/mL were transfected with 1mg/L of culture of plasmid complexed with PEI Max 40K (cat. 24765-100, Polysciences) in a 1:2 plasmid:PEI w/w ratio in 40mL Opti-MEM (cat ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...