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Citations for Polysciences :
1 - 50 of 385 citations for Mouse RTF1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with shRNA constructs and packaging plasmids (psPAX2 and VSV-G) using PEI (polyethylenimine MW25,000, Polysciences). Viral supernatants were collected at 48h and 72h post-transfection and mixed ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μg of human PANX1 plasmid and 25 μg of mouse Src Y529F mutant plasmid was used) was mixed with 150ug of PEI 25K (Polysciences) in a and incubated at room temperature for 30 min ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... pMD2-VSV-G and pLKO-shRNA or pWPI expressing NS4B using 25 kDa linear polyethylenimine (Polysciences Inc.) exactly as before (Anton et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... and 12 μg of pLKO-shRNA-puro using 63 μL of polyethylenimine (PEI – 1 μg/μL; Polysciences - 24765) mixed in 750 μL of 10 mM HEPES pH 7.4 and 150 mM NaCl in water ...
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bioRxiv - Molecular Biology 2023Quote: ... along with pLL3.7GW vector containing shRNAs for control (target sequence: CAACAAGATGAAGAAGAG CACCA) or Bmal1 (target sequence: GGAAGGATCAAGAATGCAA) using polyethylenimine (Polysciences Inc., #23966) 68 ...
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bioRxiv - Cell Biology 2020Quote: ... PlatE cells were co-transfected with the packaging plasmid pMRX-puro-tfLC3 plasmid and the envelope-encoding plasmid pVSVG by using of polyethylenimine (Polysciences Inc.). Supernatants were harvested 48 h post-transfection and filtered.
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bioRxiv - Cancer Biology 2023Quote: ... 293 T cells were cultured to a degree of 80%-90% and co-transfected with the three-plasmid system (packaging vectors psPAX2, envelope plasmid pMD2.G, and CAR plasmid) using polyethyleneimine (Polysciences, PEI MAX 40000). At 6-8 h of post-transfection ...
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bioRxiv - Neuroscience 2024Quote: ... the plasmid and helper plasmid were transfected into HEK293T cells using polyethylenimine (# 24765, Polysciences). The virus was harvested after 72 h and purified from benzonase-treated cell lysates via an iodixanol density gradient (OptiPrep ...
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bioRxiv - Genetics 2023Quote: ... plasmid using polyethylenimine (PEI, Polysciences). The cell culture medium was exchanged 12 h after transfection and the virus was harvested 24 h later ...
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bioRxiv - Cancer Biology 2024Quote: ... packaging plasmid using polyethylenimine (Polysciences). Sodium butyrate (Sigma Alrich ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with AAV plasmid and helper plasmid using PEI Max 40000 (PolySciences, USA). Twenty-four hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... plasmids were transfected using polyethylenimine (Polysciences).
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bioRxiv - Bioengineering 2023Quote: ... Plasmid DNA and polyethyleneimine (PEI, Polysciences) were independently diluted to 0.05 and 0.1 mg/mL ...
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bioRxiv - Immunology 2023Quote: ... 4µg pMDG.2 plasmid and 4µg psPAX plasmid by using the transfection reagent Polyethylenimine (PEI) (Polysciences #23966-100) at a PEI:DNA ratio of 4:1 ...
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bioRxiv - Immunology 2020Quote: ... Plasmids were transfected using polyethylenimine (Polysciences, Inc.) into 293T cells at a ratio of 1:1.5:1 of pCMVi/pCNCG/pl.18-CVS-11 ...
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bioRxiv - Cell Biology 2023Quote: ... complexes of plasmid and PEI MAX (Polysciences) were generated by mixing the appropriate pTT5 expression vector with transfection reagent (ratio of 1 µg vector ...
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bioRxiv - Microbiology 2022Quote: ... carrying defective envelope of VSV plasmid (trans form) and GFP reporter plasmid by using Transporter TM5 transfection reagent (PEI, 40,000Da, PolySciences), according to the provider guidelines ...
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bioRxiv - Biophysics 2019Quote: ... 1.4 mg plasmid A and 0.6 mg plasmid B were pre-incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences) in 50 ml fresh medium for 20 min ...
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bioRxiv - Immunology 2023Quote: ... luciferase encoding reporter plasmid (pTG126) and plasmid encoding Wuhan strain SARS-CoV-2 S using 24 μL polyethylenimine (Polysciences) in Optimem (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Microbiology 2020Quote: ... were transfected with the plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) using polyethylenimine (Polysciences, Inc.) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Bioengineering 2022Quote: ... A concentration of 2 μg mL-1 plasmid DNA and 2 μg mL-1 plasmid DNA linear 25kDa polyethyleneimine (Polysciences) dissolved in OptiPRO serum free medium (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviruses were produced by transfection of HEK 293T cells with VSV.G and gag-pol plasmids together with the plasmid of interest and PEI (2ug per ug of DNA, Polysciences Inc) in Opti-MEM medium (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... one day after plating 2ug of each plasmid (maximum 8 ug of plasmid) was transfected into cells using 60 ug of PEI max (Polysciences). Producer cells were then maintained for 3 days after transfection ...
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bioRxiv - Biophysics 2023Quote: ... the cells were transfected with WT hVMAT2-eGFP or the mutation plasmids (2μg plasmid per construct) at a ratio of 4μL polyethylenimine (PEI, 1 μg μL−1, Polysciences, 24765-1) per μg of DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were co-transfected with LentiCRISPR v2 plasmids along with packaging plasmids psPAX2 and pMD2.G using PEI transfection reagent (#23966-100, Polysciences). Lentiviruses were collected 48 hours after transfection ...
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bioRxiv - Genomics 2019Quote: The plasmids transient transfection using polyethyleneimine (PEI; Polysciences) has been detailed elsewhere10.
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bioRxiv - Molecular Biology 2019Quote: ... plasmids were transfected using PEI MAX (Polysciences Inc). For co-transfection of ILF2 siRNA with REL plasmids ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections were performed using polyethyleneimine (Polysciences, Inc.) for HEK293T cells or Lipofectamine LTX (Life Technologies Corporation ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmid transfections were performed using polyethyleneimine (Polysciences, Inc.) for HEK293T cells or Lipofectamine LTX (Life Technologies Corporation ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids and linear polyethylenimine (PEI; MW = 25000, Polysciences) were premixed and incubated at room temperature for 25 min in the following amounts per plate ...
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bioRxiv - Biochemistry 2021Quote: ... and AceTAG lentiviral plasmids using PEI (Polysciences Inc). Viral particles were collected 72 h after transfection and filtered through a 0.45 μM membrane ...
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bioRxiv - Neuroscience 2022Quote: ... and transfer plasmids using polyethylenimine MAX (Polysciences, 24765). The cell pellets were suspended in 1X Gradient Buffer (10mM Tris-HCl pH=7.6 ...
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bioRxiv - Neuroscience 2022Quote: ... and envelope plasmids using PEI (Polysciences, 24765-100). Media from transfected cells was harvested at 24 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were transfected with PEI MAX (Polysciences Inc). Primers used for this experiment are listed in Table S1 ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids and PEI (product #260008-5; Polysciences) were separately diluted in Opti-MEM1 (product #31985-047 ...
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bioRxiv - Developmental Biology 2024Quote: ... and the corresponding packaging plasmid and polyethyleneimine (Polysciences). After 72 hours ...
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bioRxiv - Biochemistry 2020Quote: ... were transiently transfected with a mixture of plasmid DNA diluted in PBS (0.67 μg total plasmid DNA per ml of culture) and Polyethylenimine (PEI) (Polysciences, Inc., 23966) at a DNA-to-PEI ratio of 1:3 ...
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bioRxiv - Molecular Biology 2023Quote: ... Producer cells were transfected with a solution containing the selected lentiviral vector transgene backbone, the packaging plasmids pMDLg/pRRE and pCMV.REV, pMD2.G (Plasmid Factory, Bielefeld, Germany) and polyethylenimine (PEI) (24765, Polysciences, Warrington, US). Transfection media was changes after 4 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pCMV-VSV-G-RSV-Rev (RIKEN BioResource Research Center plasmid RDB04393, provided by Dr. Miyoshi, Keio University) plasmids 45 using a polyethlenimine “Max” (Polysciences Inc.). Virus-containing media were collected 48 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 5.7 μg/150 mm dish of construct DNA was transfected with 22.8 μg/150 mm dish of pAAV9 capsid plasmid and 11.45 μg/150 mm dish of pAdDeltaF6 helper plasmid using polyethylenimine 25K MW (Polysciences, 23966-1). Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with TurboID-CpOGACD/DM plasmid with the packaging plasmids pPAX2 and pMD.2G using Polyethylenimine Linear (PEI, Polysciences, 24765). The PEI-containing medium was replaced with fresh serum-containing DMEM medium after 8 h ...
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Cell Biology 2022Quote: ... plasmids were transfected with polyethyleneimine (PEI; Polysciences, Cat# 23966) according to the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: ... Transfection of the plasmid was performed using PEImax (Polysciences Europe GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mg plasmid and 3 mg PEI25k (Polysciences, Inc.) was mixed in 100 ml OptiMEM media (Invitrogen) ...
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bioRxiv - Genetics 2022Quote: ... and transfer plasmids using polyethylenimine (PEI) MAX (Polysciences, 24765). Cells were harvested by cell lifter (Biologix ...