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Citations for Polysciences :
1 - 50 of 89 citations for Mouse Anti Nipah Virus Glycoprotein G AE6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Bioengineering 2021Quote: ... 2-aminoethyl methacrylate (AEMA, 3.04 g, Polysciences) (molar ratio of NHS:EDC:AEMA = 1:2:1 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Bioengineering 2021Quote: ... psPAX2 and PMD2.G using polyethyleneimine (Polysciences, Inc.) as described previously1 ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Immunology 2022Quote: ... and pMD2G-VSV-G (envelope) using PEI-MAX (Polysciences). Packaged lentivirus was used to transduce 293T cells in the presence of 5μg/mL polybrene ...
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bioRxiv - Developmental Biology 2020Quote: ... psPAX2 and pMD2.G using PEI (Polysciences, 24765-2) after reaching 70-80% confluence ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2.G using PEI Max 40000 (PolySciences, USA). The supernatant containing lentiviral particles was harvested and mixed with forty percent PEG 8000 (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2021Quote: ... and 1 μg of pHEF-VSV-G into 70% confluent monolayers of 293T cells in a 10 cm dish using polyethylenimine (Polysciences Inc, Warrington, PA.) at 4X total transfected DNA in 800 μl 150 mM NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Poly(ethylene glycol) methacrylate (PEGMA, 400 g mol-1, Polysciences) and 2-hydroxyethyl methacrylate (HEMA ...
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bioRxiv - Bioengineering 2023Quote: ... Poly(ethylene glycol) dimethacrylate (PEGDMA, 400 g mol-1, Polysciences) was used as the crosslinker (chemical structure shown in figure 1 ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of plasmid and 3 ¼g of polyethylenimine (MW 25,000; Polysciences) were mixed in 100 ¼ of OptiMEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Cell Biology 2020Quote: ... together with packaging plasmids pCMV-VSV-G and pCMV-delta8.9 using PEI (Polysciences Inc.) 48 hours after transfection ...
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bioRxiv - Cell Biology 2021Quote: ... and pMD2.G were co-transfected into 293T cells using polyethyleneimine (PEI, Polysciences 23966-1). Virus-containing supernatant media were harvested 48 hours post transfection and passed through 0.45 µm filters ...
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bioRxiv - Microbiology 2019Quote: ... and pVSV-G into 293T cells via polyethylenimine HCl MAX (PEI, Polysciences, Cat No: 24765) diluted in Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 9 µg transfer vector and 2 µg pVSV-G using 60 µl linear PEI (Polysciences). Medium was exchanged 6-8 hours post-transfection and harvested after another 48 hours ...
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bioRxiv - Microbiology 2021Quote: ... pQC-Fluc and pCAGGS-SARS2-SFmut-cflag or pCAGGS-VSV-G using PEI 40K (Polysciences, Inc.), and the medium was refreshed 6 hours later ...
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bioRxiv - Synthetic Biology 2023Quote: ... and pMD2.G along with the Lentivector (described above) and PEI (1µg/µl, polyethyleneimine) (Polysciences, USA), Lipofectamine 2000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Microbiology 2022Quote: ... 2 μg pcDNA3.1- hsa_circ_0001400 of plasmids was transfected to 2×106 cells with 12 μl Transporter 5 (Polysciences) lipofection reagent and 1 ml Opti-MemI in 10 ml DMEM ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... pMD2-VSV-G and pLKO-shRNA or pWPI expressing NS4B using 25 kDa linear polyethylenimine (Polysciences Inc.) exactly as before (Anton et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... was transfected into each 10 cm dish of HEK293T cells together with the packaging vectors psPax2 (7.5 μg) and pCMV-VSV-G (5 μg) using PEImax (67.5 μg; Polysciences) or Lipofectamine-2000 (50 μl ...
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bioRxiv - Bioengineering 2021Quote: ... the quadriceps muscle defects were filled directly with 30 mg of a synthetic (PCL, particulate, Mn = 50,000 g/mol; mean particle size <600μm, Polysciences) or biological (ECM ...
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bioRxiv - Bioengineering 2020Quote: ... PCL was employed as a synthetic material (particulate, Mn = 50,000 g/mol, mean particle size < 600 μm, Polysciences). In turn ...
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bioRxiv - Cell Biology 2019Quote: ... The contents of the 24-well plate were then spinoculated by centrifugation at 13°C for 2 hours at 1200 × g onto the coverslips and subsequently fixed in a solution of 0.1 M PIPES with 3.7% formaldehyde (Polysciences) for 15 minutes and washed 3x with PBS ...
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bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were cultured in T75 flasks and transfected at approximately 70% confluency with 7500 ng of the lentiviral vector of interest and packaging plasmids pCMV-dR8.91 (6750 ng) and pCMV-VSV-G (750 ng) with 75 µL of polyethyleneimine (PEI, 1 mg/mL; Polysciences). Approximately 72 hours after transfection ...
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bioRxiv - Genomics 2020Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
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bioRxiv - Bioengineering 2022Quote: ... Defects were filled with 30 mg of (poly)caprolactone (particulate, Mn=50,000 g/mol, mean particle size < 600um, Polysciences), decellularized extracellular matrix (Matristem ...
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bioRxiv - Microbiology 2021Quote: ... the pLHCX or pQXCIP packaging vector of interest and pCMV VSV-G using 1mg/ml PEI®-MAX (Polysciences). Media was replaced 16 h post-transfection and viral supernatants were harvested through a 0.45 µm filter ...
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bioRxiv - Cancer Biology 2021Quote: All lentiviruses were produced by co-transfecting lentiviral backbones with packaging vectors (delta8.2 and VSV-G) into 293T cells using PEI (Polysciences). The viral-containing supernatant was collected at 48- and 72-hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: ... HIV-1 were obtained by transfection of HEK293T cells with HIV-1 proviral DNA along with a VSV-G expression vector (pMD.G) and polyethylenimine (PEI) (Polysciences). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were transfected with shRNA constructs and packaging plasmids (psPAX2 and VSV-G) using PEI (polyethylenimine MW25,000, Polysciences). Viral supernatants were collected at 48h and 72h post-transfection and mixed ...
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bioRxiv - Cell Biology 2023Quote: ... lentiviral vector was co-transfected with pSPAX2 and pMD2.G in 293T cells using 40-kDa linearpolyethylenimine (Polysciences, 24,765). Retrovirus generation was by using PLAT-A cells (Cell Biolab) ...
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bioRxiv - Molecular Biology 2023Quote: HEK 293T cells were transfected with lentiviral constructs and packaging plasmids (psPAX2 and pMD2.G) using PEI transfection (Polysciences). Two days post-transfection ...
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bioRxiv - Biochemistry 2023Quote: Cells were transfected with plasmid DNA (0.75 µg) using a 1:3 (w/v) ratio of DNA to polyethyleneimine “MAX” MW 40,000 (1 mg/ml, Polysciences). These were first individually diluted in 100 µl of 150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... transfection was carried out using 0.5 μg of plasmid DNA and 2.5 µL of 1 mg mL−1 polyethylenimine (PEI) (Linear, MW 25,000, Polysciences Inc.). Twenty-four hours after transfection ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...