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Citations for Polysciences :
1 - 50 of 55 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested five days after transfection ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 or 293T cells were performed using polyethylenimine (PEI) (Polysciences) transfection ...
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bioRxiv - Microbiology 2020Quote: ... and MERS CoV lacking the endoplasmic retention signal sequence were cloned into pCAGGS expression system (pCAGGS-SARS2-S, pCAGGS-SARS-S, and pCAGGS-MERS-S) and were transfected using Polyethylenimine (PEI) (Polysciences, USA) at 1:3 ratio in HEK293T cells ...
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bioRxiv - Neuroscience 2020Quote: IgSF8-Fc and Tenascin-R-Fc proteins were produced by transient transfection of HEK293T cells using PEI (Polysciences). Six hours after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 and U2OS cells were transiently transfected using PEI-Max Transfection reagent (Polysciences, 24765) and optimem (gibco ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transiently co-transfected with lentiviral vectors using PEI MAX reagent (Polysciences, Warrington, PA, USA). Four hours post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Immunology 2021Quote: ... 1.4 µg of WT-SARS-CoV-2 spike plasmid (2) and 60 µg of PEI-Max (Polysciences). Supernatants were harvested 48h later ...
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bioRxiv - Immunology 2022Quote: ... HEK293T cells were transfected with 5μg of SARS-CoV-2 spike plasmids using PEI-MAX 40,000 (Polysciences) and incubated for 2 days at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Molecular Biology 2023Quote: ... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Microbiology 2021Quote: A pME18S expression plasmid containing the full-length or subunit spike protein was transiently transfected into HEK293T cells using PEI max (Polysciences); the pMx-GFP expression plasmid was used as the marker of transfected cells.
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T cells were co-transfected in a 2:1 ration with the pNL4-3-inGluc vector and the spike plasmid of interest using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) to produce pseudotyped lentiviral particles ...
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bioRxiv - Biochemistry 2023Quote: ... 500 μg of each plasmid encoding spike-proteins used in this study were incubated with 1.5 mg of polyethylenimine (Polysciences, Inc.) for 20 min and the mixture was deposited in 1L of HEK293F cells ...
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bioRxiv - Immunology 2020Quote: ... or pαH-SARS-CoV-2 RBD-Fc using 2 mg polyethylenimine (Polysciences, Cat# 24765). 6 days after transfection ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 Spike gene expression vector (Wuhan, Alpha, Delta, or Omicron) together with p8.9171 and pCSFLW72 using polyethylenimine (PEI, Polysciences, Warrington, USA). HIV (SARS-CoV-2 ...
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bioRxiv - Immunology 2024Quote: ... HEK293T cells were transfected with the appropriate SARS-CoV-2 spike gene expression vector in conjunction with lentiviral vectors p8.91 and pCSFLW using polyethylenimine (PEI, Polysciences, Warrington, USA). Pseudotype-containing supernatants were harvested 48 hours post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences). For intracellular transcription factor staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences).
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with PBS containing 1% FCS and fixed with 1% formaldehyde (Polysciences) and acquired on a BD LSR Fortessa using BD FACS Diva.
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bioRxiv - Immunology 2021Quote: ... The plasmids of 9μg pHAGE-luciferase-GFP、 psPAX2 and Zhou-COVID-19-Spike were co-transfected into HEK293T cells by using 1 μg/mL polyetherimide (Polysciences, Warrington, PA) in DMEM medium containing 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... A549-ACE2-A2402 cells (3 × 105 cells) were transfected with 2 μg of plasmids expressing prototype spike or its derivatives using PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Potential receptor proteins with C-terminal Flag tag were transiently expressed in HEK293F cells using polyethylenimine (PEI, Polysciences). 36 h following transfection ...
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bioRxiv - Cancer Biology 2020Quote: ... complemented with 10% FCS and 2mM glutamine) were transfected using 4μg polyethylenimine (PEI, Polysciences Inc., Warrington, USA) per μg plasmid DNA ...
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bioRxiv - Immunology 2020Quote: ... The two plasmids expressing IgA1-Fc and J-chain were co-transfected into the cells using polyethylenimine (Polysciences). Four days after transfection ...