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Citations for Polysciences :
1 - 50 of 57 citations for MAN1B1 Human HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... HEK 293 cells were cultured in ten 15 cm plates and polyethylenimine (Polysciences) transfected with an AAV plasmid overexpressing a candidate gene ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293 cells (ATCC) were transfected with plasmids using PEI ‘MAX’ (Polysciences Inc.) and subjected to electrophysiological assays 16-72 h after transfection ...
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bioRxiv - Cell Biology 2022Quote: Transfection of HEK 293 cells with plasmid DNA was performed with PEI reagent (Polysciences) in a 1:1 (w/w ...
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bioRxiv - Cell Biology 2021Quote: ... Briefly sub-confluent HEK 293 cells were transfected with branched or linear PEI (Polysciences, Inc.) 18 to 24 hrs after transfection the cells were lysed and incubated with CycB1 antibody overnight at 4°C and Protein G Sepharose beads (Sigma ...
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bioRxiv - Immunology 2022Quote: ... the pCAGGS-RBD plasmid was transfected into ∼5×107 adherent HEK-293/T17 cells (ATCC CRL-11268) in a T-175 using PEI (Polyethylenimine, linear 25,000 m.w.(Polysciences, Warrington, PA, USA). Plasmid was mixed at a 1:3 ratio with PEI (20ug of plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... Stable cell lines expressing N-terminal Flag tagged receptor constructs in pcDNA3.1 vector were generated by transfecting HEK-293 cells with 7 μg of plasmid DNA using polyethylenimine (PEI) (Polysciences, Cat. no. 19850), followed by selection using G418 (Gibco ...
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bioRxiv - Immunology 2021Quote: ... Env plasmids were co-transfected in Human Embryonic Kidney 293T or GnT1-293S cells using polyethyleneimine (PEI Max, Polysciences, Inc.), along with the MuLV Gag plasmid (23 ...
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bioRxiv - Biochemistry 2022Quote: Recombinant truncated human TSEN complexes were produced in human embryonic kidney (HEK) 293F cells by cotransfection using linear polyethyleneimine (PEI) (MW 25000, Polysciences) and incubated for 72 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Fusion proteins were transiently expressed in 293 human embryonic kidney cells (FreeStyle® 293-F) by transfecting plasmid DNA into the cells using polyethyleneimine reagent PEI MAX (Polysciences, Inc.). Transfected cells were grown in FreeStyle® 293 Expression Medium (ThermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... HEK-293T cells were transfected using homemade polyethylenimine (Polysciences, Inc.). 36h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... FreeStyle 293-F cells were transiently transfected using polyethyleneimine (Polysciences, #23966) in Opti-MEM Reduced Serum Medium (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and HEK-293T cells were transfected using polyethylenimine (#23966-1, Polysciences), while the RPE-1 cells were transfected using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were cotransfected to HEK Expi293 cells by polyethylenimine (Polysciences) at 37°C for 72 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid transfection into HEK 293T cells was carried out using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: HEK-293T cells were transiently transfected with plasmid DNA using polyethylenimine (Polysciences) at a ratio of DNA 1:3.4 polyethylenimine ...
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bioRxiv - Biophysics 2023Quote: ... Lentivirus was produced by transfecting HEK 293T cells with polyethylenimine (Polysciences Inc.) and packaging vectors (psPAX2 ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293F cells were transfected using 40 kDa polyethylenamine (PEI-MAX) (Polysciences) at 1 mg ml−1 ...
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bioRxiv - Microbiology 2024Quote: ... HEK 293F cells were transfected using 40 kDa polyethylenamine (PEI-MAX) (Polysciences) at 1 mg ml−1 ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... HEK cells were transfected using Polyethylenimine (PEI) (1mg/mL) (Polysciences, cat# 23966-2). 3µg plasmid DNA (6-well dishes ...
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bioRxiv - Immunology 2021Quote: ... Plasmid delivery in HEK-TLR4 cells was done using linear polyethyleneimine (PEI) 25K (Polysciences) complexed with DNA in a 2:1 ratio respectively for 24 h.
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bioRxiv - Microbiology 2022Quote: ... HEK 293T cells (ATCC) were reverse- transfected with polyethylenimine (PEI, Polysciences, Warrington, PA, USA) and the following plasmids for lentiviral generation ...
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bioRxiv - Cell Biology 2019Quote: HEK-293T and HeLa cells were transiently transfected with plasmid DNA using polyethylenimine (PEI, Polysciences) at a final concentration of 5 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... into HEK 293T cells using 1mg/mL of PEI Max 40000 (Polysciences, cat. 24765-1). After 48 hours of transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained in FreeStyle 293 medium and transfected using the PEI Max reagent (Polysciences Inc.) with a 1:3 ratio (w/w ...
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bioRxiv - Immunology 2022Quote: ... cultures of FreeStyle 293 (Thermo) cells were transiently transfected with the constructs using PEI MAX (Polysciences) at a 1:4 (w/w ...
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bioRxiv - Biochemistry 2020Quote: ... The expression construct was transiently transfected in HEK 293F cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Biochemistry 2021Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... The expression construct was transiently transfected in HEK 293T cells using polyethylenimine (Polysciences, Inc, Warrington, PA). Protein was purified from cell supernatants using Ni-NTA resin (Qiagen) ...
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bioRxiv - Microbiology 2020Quote: ... triple plasmid transfection of 293 adherent cells was performed with a polyethylenimine (PEI)-MAX (Polysciences, MW, 40,000) to DNA ratio of 3:1 (a mass-per-mass ratio) ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T or GP2-293 cells were used for lentivirus or retrovirus production using polyethyleneimine (PEI 25000, Polysciences). Supernatants containing lentiviral and/or retroviral particles were applied to RPE1-Tet3G-derived host cells for 24h ...
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bioRxiv - Cell Biology 2020Quote: ... The Ecad and Dsc3 plasmids were transiently transfected into HEK 293T cells using Polyethylenimine (PEI) (Polysciences, Inc.). Four days post transfection ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293T cells were transiently transfected with pLVX-IRES-Puro-WDR5 plasmid by PEI (Polysciences, 23966-2) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK-293T cells were co-transfected with 100 ng of corresponding plasmids using linear PEI (Polyethyleneimine, Polysciences). 24h after transfection ...
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bioRxiv - Biochemistry 2023Quote: HEK cells were transiently transfected at 80% confluency with CibN and CryC plasmids using polyethylenimine (PEI) (Polysciences, Inc.). Plasmids and PEI were diluted in Opti-MEM (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: HEK 293T cells were transfected with lentiviral constructs and packaging plasmids (psPAX2 and pMD2.G) using PEI transfection (Polysciences). Two days post-transfection ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2022Quote: Secreted IgG was produced by co-transfecting 106 293F cells/mL with the paired heavy and light chain expression plasmids at a ratio of 1:1 in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Fc.IL-2-expressing plasmids (pcDNA3.1) were transiently transfected into HEK 293T/17 adherent cells (ATCC CRL-11268) using PEI 25K (Polysciences, Inc.) (ratio 3:1 ...
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bioRxiv - Molecular Biology 2022Quote: Proteins were produced by transient transfection of plasmids into HEK 293F cells using the polyethylenimine (PEI) Max reagent (Polysciences, Inc.) with a 1:3 ratio (wt/wt ...
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bioRxiv - Biophysics 2020Quote: ... the plasmids pCAGGS-SARS2-S and pCAGGS-SARS-S were transiently expressed on HEK 293T cells using Polyethylenimine (PEI) (Polysciences, USA). 24 h post-transfection ...
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bioRxiv - Microbiology 2022Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences, cat#24765). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Cancer Biology 2019Quote: Lentivirus was produced in HEK 293T cells by transfecting plasmid DNA and packaging plasmids (VSVG and psPAX2) using Polyethylenimine (PEI 25000; Polysciences; Cat# 23966-1). Media was replaced with target media 6-8 hours following transfection and lentivirus-containing supernatant was subsequently collected every 12 hours for 48 hours prior to filtration through a 0.45µm filter ...
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bioRxiv - Neuroscience 2023Quote: ... and 30 ug pHelper plasmid into one 15-cm dish of confluent HEK-293T cells using PEI-Max (Polysciences Inc. catalog # 24765-1). After transfection the next day we changed the medium to 1% FBS ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...