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Citations for Polysciences :
1 - 50 of 158 citations for IL 5 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested five days after transfection ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... the cells were transiently transfected with 5 μg of pcDNA3L-His-CAMYEL plasmid using the linear polyethylenimine (PEI, m.w. 25 kDa) (Polysciences, Warrington, PA, USA). The PEI/DNA complex mixture was sequentially added to the cells at the ratio of 1:6 ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 or 293T cells were performed using polyethylenimine (PEI) (Polysciences) transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 and U2OS cells were transiently transfected using PEI-Max Transfection reagent (Polysciences, 24765) and optimem (gibco ...
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bioRxiv - Plant Biology 2020Quote: Control and extract-treated (for 30 min or 1 h) root tips of 4- to 5-day-old rice seedlings were fixed in 3% (v/v) glutaraldehyde (PolySciences, Niles, IL, USA) in 50 mM sodium cacodylate buffer (pH 7 ...
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bioRxiv - Immunology 2023Quote: ... Picrosirius red (PolySciences 24901-500; Niles, IL) was used for collagen staining ...
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transiently co-transfected with lentiviral vectors using PEI MAX reagent (Polysciences, Warrington, PA, USA). Four hours post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Synthetic Biology 2022Quote: ... Transporter 5™ reagent (Polysciences, Inc, Cat #26008-5) was used to transfect parental HEK293T/17 cells ...
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bioRxiv - Biochemistry 2023Quote: ... -MCM9 M45E or -GFP control (Transporter 5, Polysciences 26008-5) and harvested 3 days later for HA-immunoprecipitation as previously described39 ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant proteins fused with either Flag or Flag-HA-His tags were transfected at 20 µg per 15 cm plate using Polyethyleneimine 25000 (23966-100, Polysciences) solution at 2.5 µg per 1 µg of DNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Fast Blue (5%, Polysciences) or rAAV-hSyn-EGFP (1012vp/ml ...
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bioRxiv - Neuroscience 2021Quote: ... or Aquamount (#18606-5, Polysciences). Immunofluorescence was analyzed using a Nikon Eclipse Ti-E inverted microscope (Nikon Instruments Europe B.V. ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The day after seeding, pcDNA3L-His-CAMYEL plasmid were transiently transfected into cells using linear polyethyleneimine (PEI, m.w. 25 kDa) (#23966, Polysciences, Warrington, PA, USA). The DNA-PEI complex mixture was added into the cells at the ratio of 1:6 ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were transfected with 100 ng/well of pcDNA 3.1 Myc/His empty vector or MT1-MMP plasmid DNA using PEI max transfection reagent (Polysciences, catalog no. 24765). After 6 hours of treatment ...
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bioRxiv - Cancer Biology 2021Quote: ... 5 μL PEI (Polysciences, #23966-1) in 50 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Fluorescent polychromatic beads (Polysciences, 19507-5) with a 0.5 μm particle size were added to each well at a final concentration of 3.64×108 beads/mL and incubated at 20°C for 10 minutes with shaking ...
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bioRxiv - Microbiology 2020Quote: ... pCAG-HIVgp (5 μg) and pCMV-VSV-G-RSV-Rev (5 μg) (Riken BioResource Research Center) using PEImax solution (60 μL) (Polysciences) prepared in KnockoutDMEM/F-12 (500 μL ...
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bioRxiv - Biochemistry 2020Quote: ... 5 µm Polybead carboxylate microspheres (Polysciences, Warrington, PA) were covalently coupled with goat anti-human pAb using 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide/EDC chemistry (49) ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids and PEI (product #260008-5; Polysciences) were separately diluted in Opti-MEM1 (product #31985-047 ...
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bioRxiv - Cell Biology 2024Quote: ... Polystyrene microspheres (Polysciences #00876-15; 5-fold dilution) were used to immobilize late-L4 animals on 10% agarose pads ...
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bioRxiv - Cancer Biology 2022Quote: Calculation of absolute cell number was performed by adding to each vial a fixed number (10,000) of nonfluorescent 10 μm polybead® carboxylate microspheres (Polysciences, IL, USA) according to the formula ...
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bioRxiv - Plant Biology 2020Quote: ... Dehydration was performed in an acetone series, followed by treatment with propylene oxide (SERVA, Heidelberg, Germany) at 4°C and embedding in Spurr’s resin (PolySciences, Niles, IL, USA). The embedded specimens were sectioned with a Reichert-Jung Ultracut E (Reichert-Jung Optical Company ...
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bioRxiv - Molecular Biology 2022Quote: ... All human PBAF subunits were co-transfected into suspension Expi293F cells using polyethylenimine (Polysciences). Cells were cultured for 72 h at 37°C and harvested by centrifugation ...
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bioRxiv - Biophysics 2022Quote: ... 5% agarose pads and 0.1 μm polystylene beads (Polysciences) as described (Niwa ...
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bioRxiv - Cancer Biology 2021Quote: ... using 5 μL of Polyethylenimine (PEI) (Polysciences, 23966-1) at 1 mg/mL stock concentration ...
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bioRxiv - Immunology 2023Quote: ... at 5 ×106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...
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bioRxiv - Genomics 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery96 ...
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bioRxiv - Immunology 2023Quote: ... at 5 x106 cells/mL using PEI MAX (Polysciences) for DNA delivery ...