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Citations for Polysciences :
1 - 50 of 432 citations for Estriol 2 4 16 17 D4 98% 95% Pure since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: Ovaries were isolated from Drosophila melanogaster adult females straight into fixation medium and fixed in 4% PFA (prepared fresh from a stock solution of 16% paraformaldehyde, methanol free ultra - pure EM grade, Polysciences) in Cytoskeleton Buffer [21] ...
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bioRxiv - Microbiology 2023Quote: ... FACS buffer was removed and pellets were fixed in freshly prepared 2% methanol free formaldehyde (diluted from 16% Ultra Pure EM grade formaldehyde (Polysciences, Warrington, PA, USA) for a minimum of 1 hour prior to analysis ...
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bioRxiv - Microbiology 2020Quote: ... then fixed in 4% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA) diluted in PBS for 10 min at room temperature followed by a 5 min incubation with 250 mM glycine to stop the fixation reaction ...
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bioRxiv - Immunology 2023Quote: ... and PEI (Polysciences, 9002-98-6), and transfected into HEK293T cells at 80% to 90% confluence in 10-cm tissue culture plates ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed and fixed with 2% ultra-pure formaldehyde (Polysciences, Warrington, PA) for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Spheroids in the Elplasia plate were then placed on ice and fixed in situ for 20 minutes on ice in 1% effective Formaldehyde added to the serum-free media (Formaldehyde, 16%, methanol-free, Ultra Pure; Polysciences #18814-20). The cell-fixation media was then removed and the cells were incubated with 50 mM TrisHCL (Thermo Scientific #J22638.AE ...
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bioRxiv - Immunology 2023Quote: ... For assessment of phosphorylated proteins cells were fixed in 4% ultra-pure formaldehyde (#18814-20, Polysciences) for 10 minutes at 37°C directly after cell-culture ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Bioengineering 2019Quote: Neurons were fixed in methanol-free 4% formaldehyde ultra-pure EM grade (Polysciences, Inc., cat. no. 18814-10) + 4% sucrose in PBS for 20 min at room temperature and permeabilized with 0.2% Triton X-100 for 5-10 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... N-(3-Aminopropyl) methacrylamide hydrochloride (APMA, >98%) was obtained from Polysciences Inc ...
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bioRxiv - Molecular Biology 2019Quote: ... into HEK293T/17 cells with polyethylenimine (Polysciences) and collected supernatants at 48 and 72h post transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... fixed by adding equal volume of 4% formaldehyde solution (16% methanol-free formaldehyde by Polysciences, 18814, diluted in PBS) whilst vortexing to ensure single cell suspension ...
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bioRxiv - Developmental Biology 2019Quote: Flies of both genders were raised as described above and larvae from second instar to pre-pupal stages collected and dissected directly into fresh 4% EM grade paraformaldehyde solution (from a 16% stock. Polysciences) in PBS with 0.3% TritonX-100 then incubated for 25 min at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... single cell suspension was returned to a cell incubator (37°C & 5% CO2) for 1 hour in cRPMI and fixed with 4% ultra-pure formaldehyde (#18814-20, Polysciences, Hirschberg an der Bergstraβe, Germany) for 15 minutes at RT ...
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bioRxiv - Biophysics 2022Quote: ... the excess solvent was blotted and 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was added on the grid ...
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bioRxiv - Cell Biology 2019Quote: ... Tissues were counter-stained with 4’,6-diamino-2-phenylindole (DAPI, Polysciences) at 1 μg/mL ...
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bioRxiv - Biophysics 2021Quote: ... ~2 mg plasmids premixed with 4 mg linear polyethylenimines (PEIs) (Polysciences, Inc) in 45 mL fresh medium for 15 min ...
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bioRxiv - Microbiology 2019Quote: ... Cells were transfected with 4 µl of Polyethylenimine (PEI) (Polysciences cat# 23966-2) at 1 mg/ml concentration for every 1 µg plasmid DNA stock in 9ml of Opti-MEM ...
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bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Physiology 2022Quote: ... animals were mounted on 2 % agarose dry pads with 4 % polystyrene beads (Polybeads, Polysciences) in M9 buffer ...
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bioRxiv - Immunology 2023Quote: ... the remained solvent was blotted and three drops of 1% uranyl acetate (Uranyl Acetate 98%, ACS Reagent, Polysciences, Inc.) was applied on the grid for staining purpose ...
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bioRxiv - Cell Biology 2020Quote: ... in a ratio of 4:2:1 using polyethylenimine (PEI) (Cat#23966-1, Polysciences, Inc.) (PEI:DNA mass ratio 3:1) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then cross-linked with 1% formaldehyde (Ultra Pure EM grade, Polysciences, Warrington, PA), for 10 min at 26°C followed by another 5 min incubation after adding glycine to a final concentration of 125 mM to stop the cross-linking reaction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Formaldehyde (16%, methanol free) was purchased from Polysciences (Warrington, PA). Phosphate-buffered saline with (PBS) ...
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bioRxiv - Systems Biology 2023Quote: ... 33 µl of 16 % methanol-free formaldehyde (Polysciences #18814-20) was added to 100 µl of culture medium in each well to fix the cells in 4% final formaldehyde concentration ...
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bioRxiv - Cell Biology 2022Quote: ... We then rinsed the samples with pure acetone before infiltration of the resin lowicryl HM20 (Polysciences). The resin was polymerized with UV at -25 °C ...
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bioRxiv - Neuroscience 2023Quote: ... 4 µL FastBlue (FB; 2% in sterile PBS; CAS# 73819-41-7; Polysciences Inc., Warrington, PA USA) per paw was then slowly injected ...
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bioRxiv - Genetics 2019Quote: ... at a weight ratio of 4:3:2 using 54 µL PEI (Polysciences, 24765-1, 1 µg/µl). We changed the medium after 4-6 hours of incubation at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.). Mounting medium was allowed to harden overnight ...
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bioRxiv - Cell Biology 2022Quote: ... After 6 h of LPS treatment 16% Formaldehyde (18814-20, Polysciences Inc.) was added to the medium in the dish to a concentration of 1% and incubated (gently mixing ...
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bioRxiv - Microbiology 2023Quote: ... 20mM HEPES) with formaldehyde (molecular grade methanol-free 16% formaldehyde, Polysciences Inc.) at a final percentage of 1% for 30 minutes at room temperature in a vertical rotator ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with 2 µg of pCI-neo Fluc-EGFP or pCI-neo FlucDM-EGFP with 4 µg of PEI (Polysciences) and cultured for an additional 24 h ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then stained with 5 µg/ml DAPI (4′,6-diamidino-2-phenylindole) for two minutes to visualize nuclei and mounted in Mowiol mounting medium (Polysciences) in glycerol containing 2.5% 1,4-diazabicyclo-(2,2,2)-octane (DABCO ...
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bioRxiv - Neuroscience 2022Quote: ... 25 and 45 day old flies were immediately fixed after decapitation in 4% paraformaldehyde (Laborimpex) and 2% glutaraldehyde (Polysciences, Inc) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Neuroscience 2021Quote: Mice were sacrificed at 2 and 10 months of age by perfusion with 4% w/v paraformaldehyde (PFA; Polysciences, EM grade) and 0.1% v/v glutaraldehyde (GA ...
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bioRxiv - Cell Biology 2022Quote: Adult fly heads were decapitated and immediately fixed in 4% paraformaldehyde (Laborimpex, 15714) and 2% glutaraldehyde (Polysciences, Inc, 111-30-8) in 0.1 M Na-Cacodylate buffer pH 7.4 (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... The tissue slides were then dehydrated in an increasing gradient of ethanol (95%, 100%, 100%) and re-paraffinized with xylene and mounted in Poly-Mount (Polysciences, Inc.). Finally ...
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bioRxiv - Immunology 2020Quote: ... Fc.IL-2-expressing plasmids (pcDNA3.1) were transiently transfected into HEK 293T/17 adherent cells (ATCC CRL-11268) using PEI 25K (Polysciences, Inc.) (ratio 3:1 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were then transfected with ACE2-Ig-95 or ACE2-Ig-105/106 plasmid using PEI 25K (Polysciences, Cat. No. 23966-1) and selected using methionine sulfoximine (MSX)-containing EX-CELL CD CHO Fusion Medium (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... and cross-linked in 40 ml PBS with 1% formaldehyde (Formaldehyde methanol free 16%, Polysciences inc.) at room temperature for 30 min with vertical rotation at 15rpm ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Cell Biology 2020Quote: ... and cell pellets resuspended in 9.37 ml PBS plus 0.63 ml 16% formaldehyde-EM grade (Polysciences, Inc) and incubated at room temperature for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Physiology 2022Quote: ... 16 μg AAV-U6-sgRNA-TBG-Cre plasmid and 144 μl of 1 mg/ml Polyetherimide (PEI) (Polysciences) was mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Cancer Biology 2019Quote: ... and either 2-µm YG microspheres (Polysciences; 17155-2) or 15-µm scarlet microspheres (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 2 ml 2% PFA (Polysciences, Inc., 18814). After 30 min incubation at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in 4% Formaldehyde (Polysciences 18814) / PEM buffer (0.1 M PIPES ...
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bioRxiv - Microbiology 2021Quote: ... were fixed with 4% formaldehyde (Polysciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2020Quote: ... fixed in 4% paraformaldehyde (Polysciences, #18814) and mounted in Prolong Diamond to preserve GFP signal were also prepared ...