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Citations for Polysciences :
1 - 50 of 203 citations for Dengue Virus Serotype 4 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Developmental Biology 2022Quote: ... then fixed overnight at 4°C in 4% methanol-free formaldehyde (Polysciences, # 040181). Wholemount in situ hybridization (WISH ...
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bioRxiv - Neuroscience 2020Quote: ... fixed in 4% Formaldehyde (Polysciences 18814) / PEM buffer (0.1 M PIPES ...
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bioRxiv - Microbiology 2021Quote: ... were fixed with 4% formaldehyde (Polysciences) in phosphate buffered saline (PBS) ...
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bioRxiv - Biophysics 2020Quote: ... fixed in 4% paraformaldehyde (Polysciences, #18814) and mounted in Prolong Diamond to preserve GFP signal were also prepared ...
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bioRxiv - Physiology 2021Quote: ... ATP [5mM] and 4% formaldehyde (Polysciences). After fixation ...
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bioRxiv - Microbiology 2022Quote: ... 200 μl of 4% PFA (Polysciences) was added to the upper chamber of the Transwells and incubated for 30 min ...
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bioRxiv - Immunology 2023Quote: ... or 6 μm (#17145-4; Polysciences) amine-conjugated beads were washed in anhydrous DMSO (#276855 ...
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bioRxiv - Developmental Biology 2024Quote: ... and fixed with 4% formaldehyde (Polysciences) for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Klf4 or Oct-3/4 or Klf4 + Oct-3/4 or Klf4 + OctER using PEI (Polysciences, USA). Self-ligated empty pGEM-T vector (Promega ...
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bioRxiv - Microbiology 2019Quote: ... collected by centrifugation and fixed for 1 h at 4°C in 4% paraformaldehyde (Polysciences Inc., Warrington, PA) in 100 mM PIPES/0.5 mM MgCl2 ...
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bioRxiv - Cancer Biology 2019Quote: ... fixed in 4% methanol-free formaldehyde (Polysciences) in phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... followed by incubation with 4% formaldehyde (Polysciences)-PBS for 20 mins at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... fixed with 4% formaldehyde (FA; 18814; Polysciences) for 12 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4 % paraformaldehyde (Polysciences) and stained with the antibody anti-mouse KI67 ...
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bioRxiv - Developmental Biology 2020Quote: Embryos were fixed in 4% formaldehyde (Polysciences) for 10 minutes then immediately permeabilized for 30 minutes in 0.5% Triton X-100 (Millipore-Sigma ...
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bioRxiv - Developmental Biology 2019Quote: Embryos were fixed in 4% formaldehyde (Polysciences) for 10 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were fixed with 4% formaldehyde (Polysciences) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... fixed with 4% paraformaldehyde (PFA) (Polysciences, Inc.) in 1× PBS for 10 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Biophysics 2019Quote: ... 1.4 mg plasmid A and 0.6 mg plasmid B were pre-incubated with 4 mg linear polyethylenimines (PEIs) (Polysciences) in 50 ml fresh medium for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... For transfection cells were centrifuged and resuspended in fresh media to a density of 4×106 cells/mL and transfected with 4 μg plasmid/mL and 16 μg/mL of PEI 40 kDa (Polysciences). 4 h up to 24 h after transfection cells were fed with 75% of the transfection volume ...
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bioRxiv - Cell Biology 2019Quote: ... fixed for 15 min in 4% formaldehyde (Polysciences), washed 3 times with PBS and incubated 10 min with 15mM Glycine ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted in Mowiol 4-88 (Polysciences) and imaged on a Zeiss confocal microscope (LSM 880) ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Developmental Biology 2019Quote: ... After fixation with 4% PFA (Polysciences, Inc., 18814) for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected midguts were fixed in 4% Formaldehyde (Polysciences) in 1x PBS for 30 minutes at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% methanol-free formaldehyde (Polysciences) and 0.1 M potassium phosphate buffer (pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were then washed 3 × 30 mins in PBS and then immersed through a gradient of sucrose solutions from 10%-50% at 4°C for > 4 hrs each before being embedded in cryomolds (Polysciences 18646A) using OCT (Sakura Finetek ...
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bioRxiv - Biophysics 2019Quote: ... the samples were fixed with 4% paraformaldehyde (PFA; Polysciences) in 1 x PBS for 10 minutes at 25 °C and subsequently rinsed with 1 x PBS ...
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bioRxiv - Cell Biology 2022Quote: ... the COs were fixed with 4% paraformaldehyde (PFA; Polysciences) for 30 min at room temperature and subsequently embedded in cryogel (Tissue-Tek ® O.C.T ...
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bioRxiv - Cell Biology 2019Quote: ... cells were fixed with 4% EM grade Formaldehyde (Polysciences) for 5 minutes at 37 °C ...
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bioRxiv - Developmental Biology 2021Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Genetics 2019Quote: ... The samples were fixed in either 4% formaldehyde (Polysciences) for 30 minutes or in Bouin’s fixative (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... 4% (v/v) electron microscopy-grade formaldehyde (PFA, Polysciences) for 10 min on ice before and then incubated with 20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were fixed in 4% methanol-free formaldehyde (PolySciences) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... and fixed in 4% formaldehyde (Polysciences, Inc., 18814-10) in 1xPBS for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were fixed with 4% formalin (Polysciences, 04018-1), permeabilized with 0.3% Triton X-100 in 1X PBS for 20 minutes at room temperature ...