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Citations for Polysciences :
1 - 50 of 209 citations for Dengue Virus Serotype 3 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and the envelope protein expressing plasmid (VSV-G) using polyethylenimine (PEI 25K™; Polysciences; Cat. No. 23966) transfection reagent ...
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bioRxiv - Neuroscience 2022Quote: ... and envelope plasmids using PEI (Polysciences, 24765-100). Media from transfected cells was harvested at 24 ...
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bioRxiv - Immunology 2022Quote: ... and pMD2G-VSV-G (envelope) using PEI-MAX (Polysciences). Packaged lentivirus was used to transduce 293T cells in the presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Biochemistry 2021Quote: The expression vectors encoding for all S proteins were transiently transfected into HEK293 Freestyle (HEK293F) cells with polyethylenimine (PEI, linear, 25 kDa, Polysciences, USA) at a ratio of DNA ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... HEK293 cells were transfected using polyethylenimine (PEI; Polysciences) (113 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Cell Biology 2020Quote: ... envelope (pMD2-VSV) and pLenti6/V5 plasmids using linear polyethylenimine (PEI) (23966-1, PolySciences Europe ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested five days after transfection ...
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bioRxiv - Biophysics 2022Quote: ... Plasmid constructs were transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Neuroscience 2024Quote: Serotype 8 adeno-associated viruses (AAV8) were produced in HEK293FT cells grown on 5 (Polysciences Europe, 23966-100) or 10 15-cm diameter plates (Sigma-Aldrich #408727) ...
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bioRxiv - Developmental Biology 2020Quote: ... Constructs were transfected together into HEK293 cells using polyethylenimine (PEI, Polysciences) with a 24-h incubation time for protein expression ...
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bioRxiv - Neuroscience 2020Quote: ... All other AAVs were prepared in large scale HEK293T cell cultures that were transfected with mixture plasmids of AAV encoding full-length cDNAs and the serotype DJ packaging plasmids pHelper and pRC-DJ with polyethyleneimine (Polysciences). 48 h after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK293 cells were reverse transfected using poly(ethyleneimine) (linear, 20 kDA; Polysciences)49 ...
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bioRxiv - Microbiology 2020Quote: ... Transfections of HEK293 or 293T cells were performed using polyethylenimine (PEI) (Polysciences) transfection ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 and U2OS cells were transiently transfected using PEI-Max Transfection reagent (Polysciences, 24765) and optimem (gibco ...
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bioRxiv - Microbiology 2022Quote: ... carrying defective envelope of VSV plasmid (trans form) and GFP reporter plasmid by using Transporter TM5 transfection reagent (PEI, 40,000Da, PolySciences), according to the provider guidelines ...
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bioRxiv - Cell Biology 2023Quote: ... were used for lentivirus production by co-transfecting 6 μg of Lentiviral expression plasmid with 4 μg psPAX2 packaging plasmid and 0.8 μg pMD2.G envelope plasmid using the polyethylenimine transfection reagent (Polysciences). Cells with transfection medium were exchanged with fresh medium 12 hours after transfection ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Cell Biology 2020Quote: ... PlatE cells were co-transfected with the packaging plasmid pMRX-puro-tfLC3 plasmid and the envelope-encoding plasmid pVSVG by using of polyethylenimine (Polysciences Inc.). Supernatants were harvested 48 h post-transfection and filtered.
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bioRxiv - Cell Biology 2022Quote: ... COS-7 and HEK293 cells were transfected with DNA plasmids using PEI (Polysciences, Inc., Warrington, PA). At 24 hours post-transfection ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was transfected into HEK293 cells using PEI MAX 40000 (Polysciences, Inc., Warrington, PA, USA). The cultures were grown in FreeStyle 293 serum-free media (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were transfected into HeLa Kyoto and HEK293 cells using linear polyethylenimine (PEI, MW 25,000; Polysciences). One day prior to transfection ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Immunology 2022Quote: ... and packaging plasmid psPax2 and envelope plasmid pMD.2G with polyethylenimine (PEI) (at a ratio of 1:2.3 DNA:PEI) (Polysciences, catalog no. 24765-2) in Optimem (Life Technologies Europe BV ...
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bioRxiv - Microbiology 2020Quote: ... Plat-E cells were co-transfected with target gene vectors and an amphotropic envelope vector using polyethylenimine (PEI; Polysciences, Warrington, PA, USA) as described previously39 ...
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bioRxiv - Biochemistry 2020Quote: ... HDX-MS and MALS analysis were expressed in HEK293-GnT1 suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transfected with expression and packing plasmids following standard calcium phosphate or polyethylenimine (Polysciences #23966) protocols ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were transiently co-transfected with lentiviral vectors using PEI MAX reagent (Polysciences, Warrington, PA, USA). Four hours post-transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... 293 T cells were cultured to a degree of 80%-90% and co-transfected with the three-plasmid system (packaging vectors psPAX2, envelope plasmid pMD2.G, and CAR plasmid) using polyethyleneimine (Polysciences, PEI MAX 40000). At 6-8 h of post-transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant AAV vectors were produced by transfecting HEK293 cells at ~75% confluence with polyethylenimine (PEI Max; Polysciences, 24765) using a triple plasmid transfection protocol with the AAV Rep-Cap plasmid ...
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: For stable cell line generation HEK293-FT cells (4×106 in a 10 cm dish) were transfected using 4µg polyethylenimine (PEI, Polysciences) per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961 ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Biochemistry 2020Quote: ... ADP-Glo kinase assays and liposome sedimentation assays were expressed in HEK293-GnTI suspension cells by using the polyethylenimine (Polysciences) transfection system ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... with modified pCW57.1 target vector in a ratio of 5:1:5:5 into HEK293 cells using polyethyleneimine (PEI, Polysciences, 23966) as transfection reagent ...
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bioRxiv - Neuroscience 2023Quote: HEK293 or HEK293T cells were transfected with plasmids expressing GFP-fused NCAN using Lipofectamine 3000 (Thermo) or PEI Max (Polysciences). After three or four days ...
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the plasmids for G protein activation (the ratio of receptor/Gα/Gβ/Gγ was 4/1/2/8) using linear polyethyleneimine (PEI, Polysciences, 3:1 PEI:DNA ratio) (65 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S-expression plasmids (500 ng) and pDSP8-11 (500 ng) using PEI Max (Polysciences, Cat# 24765-1). To prepare target cells ...
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bioRxiv - Cell Biology 2020Quote: ... 3-micron beads (Polysciences Inc) were resuspended in 0.1 M Borate and incubated with human IgG at 4°C overnight while rotating ...