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Citations for Polysciences :
1 - 50 of 621 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells for virus generation were transfected with PEI reagent (Polysciences, US). HEK293T and DLD1 cells were kindly provided by Prof ...
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bioRxiv - Neuroscience 2023Quote: ... and virus was generated by transfecting HEK293T cells with PEI Max (Polysciences, 24765); after 48 hrs the media was collected and ultracentrifuged at 17,000 rpm at 6°C for 1 hr.
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bioRxiv - Immunology 2021Quote: Pseudotyped reporter virus was produced by transfection of 293T cells with four plasmids complexed with polyethyleneimine (Polysciences): dual-reporter vector described above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... To generate pseudotyped reporter virus the 293T cells were transfected with 4 plasmids mixed with Polyethylenimine (PEI, Polysciences): dual reporter genome vector ...
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bioRxiv - Microbiology 2022Quote: HIV virus was produced by transient transfection of HEK293T cells using Polyethyleneimine (PEI, 1 mg/ml, linear, MW 25000, Polysciences Inc.). Pseudotyping of GFP-HIV (HIV-1 NL4-3 ΔEnv EGFP Reporter Vector ...
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bioRxiv - Microbiology 2019Quote: ... Virus-like particles (VLPs) were produced by linear polyethylenimine 25K (Polysciences) transfection of HEK-293T ...
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bioRxiv - Cell Biology 2020Quote: For virus production, lentiCRISPR v2 plasmids(Sanjana et al, 2014) were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
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bioRxiv - Microbiology 2019Quote: ... HEK293T cells were seeded in 100-mm dishes and transfected with pNL4-3-lucΔVifΔEnv and vesicular stomatitis virus glycoprotein (VSV-G) using the PEI transfection reagent (Polysciences) following the manufacturer’s instruments ...
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bioRxiv - Systems Biology 2023Quote: Retrovirus was produced by transiently co-transfecting HEK 293T cells with a plasmid encoding a CAR or control construct and pRD114/pHIT60 virus-packaging plasmids (courtesy of Dr. Steven Feldman of the National Cancer Institute) using linear polyethylenimine (25 kDa; Polysciences). After 48 and 72 hours ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells grown in 10-cm dishes were transfected with the pNL4-3 and a vesicular stomatitis virus glycoprotein (VSV-G) expression plasmids at a ratio of 4:1 using polyethyleneimine (PolySciences, Warrington, PA). Cell culture supernatants containing infectious virions were collected two days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... with GRIK1-1aEM plasmid [or virus, prepared in DH10Bac as per established protocol (Goehring et al., 2014)] using polyethyleneimine (PEI-MAX, Polysciences; 1 DNA: 3 PEI w/w) as the transfection agent (or ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 ×106 cells/mL using PEI (Polyethyleneimine “MAX”, MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 48 h ...
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bioRxiv - Cancer Biology 2023Quote: ... A total of 1.1 mg DNA/L cells was co-transfected into cells at a density of 2.5 x106 cells mL-1 using PEI (Polyethyleneimine "MAX", MW 40,000, Polysciences, 24765, total 3 mg PEI/L cells). After 52 h (mTORC1 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transiently transfected at a density of 3 × 106 cells/mL using linear polyethylenimine (Polysciences). 24 h following transfection ...
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bioRxiv - Genomics 2022Quote: ... cells are transfected using PEI (Polysciences) with 1 ug of total DNA comprising 500 ng of piggyBac (fused or unfused ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected using polyethylenimine (Polysciences) or Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2022Quote: ... cells using polyethylenimine (PEI) (Polysciences, Inc.) to generate the lentiviral particles ...
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bioRxiv - Bioengineering 2020Quote: ... 0.625 μg of plasmid DNAs per million cells followed by 2.5 μg polyethyleneimine per million cells (Polysciences) were added to the cells and gently mixed ...
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bioRxiv - Cell Biology 2019Quote: ... MDCK-II cells were transfected with 1 μg plasmid DNA per 106 cells using either polyethlyenimine (Polysciences Inc) or Lipofectamine-2000 (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... DNAs were transfected cells using polyethylenimine (Polysciences). After 48-72 h expression ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected using PEI MAX (Polysciences) at a DNA:PEI ratio of 1:3.75 ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 10% paraformaldehyde (Polysciences) for 24 hours and then stained with anti-spike antibodies and plaques were counted.
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bioRxiv - Cell Biology 2022Quote: ... cells were fixed with 4 % paraformaldehyde (Polysciences) and stained with the antibody anti-mouse KI67 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 10% formaldehyde (Polysciences). The next day ...
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bioRxiv - Molecular Biology 2019Quote: ... into HEK293T/17 cells with polyethylenimine (Polysciences) and collected supernatants at 48 and 72h post transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were transfected using PEI MAX (Polysciences) at a DNA:PEI ratio of 1:3.75 ...
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bioRxiv - Immunology 2023Quote: ... Cells were transfected using PEI Max (Polysciences) and relevant expression vector ...
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bioRxiv - Microbiology 2022Quote: ... B16F10 cells (2,500,000 cells) were transfected with 5 μg S expression plasmid by PEI Max (Polysciences, Cat# 24765-1) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Calu-3 cells and HEK-293F cells were cultured and transfected using either Lipofectamine 3000 (Thermo) or polyethylenimine (Polysciences) as previously described(26 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells (4 × 106 cells/150 mm dish) were transiently transfected with pcDNA3-hFGF18-FLAG using PEI-MAX (Polysciences 24765). The culture medium was changed 24 hours after transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Transfection was performed when the cell density reached 2.5 × 106 cells per mL by using expression plasmids and polyethylenimines (PEIs, Polysciences, USA). Approximately 1.5 mg of plasmids were premixed with 4.5 mg of PEIs in 50 mL of fresh medium for 25 minutes before application ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were mounted with Aqua Poly/Mount (Polysciences) and imaged using a Zeiss LSM510 META or LSM880 laser confocal microscope under a 40x objective ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were transfected with PEI (Polysciences, #23966-2) using 15 μg of CIGAR-seq vector ...
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bioRxiv - Cell Biology 2021Quote: ... cells were transfected using linear polyethylenimine (Polysciences Inc.) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using polyethylenimine (PEI, Polysciences Inc.) and 150 mM NaCl using a 1:6 (w:v ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were embedded with Aqua-Poly/Mount (Polysciences). The primary and secondary antibodies used for immunostaining are listed in Appendix Table S3.
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were transiently transfected with polyethyleneimine (Polysciences) with different StrepII-GFP-CSPP1 constructs ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were fixed with 4% paraformaldehyde (PFA; Polysciences) for 10 minutes at 37°C and permeabilized in ice-cold 90% methanol (Merck ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T17 cells were transfected using polyethylenimine (PEI, Polysciences) with helper plasmid pxx-680 (A gift from R.J ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were transfected using Polyethylenimine MAX (Polysciences Inc.) or 293fectin (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The cells were fixed with 10% formaldehyde (Polysciences) for 24 hours after which the ELISA procedure mentioned above was performed using serum from each vaccinated animal.
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bioRxiv - Biochemistry 2021Quote: ... and transfected into HEK293 cells using polyethyleneimine (Polysciences). Cell growths were harvested four days after transfection ...
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bioRxiv - Biochemistry 2021Quote: WT HEK293T cells were transfected with polyethylenimine (Polysciences) using a DNA to polyethylenimine ratio of 1:2.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Constructs were transfected to cells using polyethylenimine (Polysciences). After 60 h of expression ...
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bioRxiv - Genomics 2020Quote: ... Cells were transfected using the PEI method (Polysciences). Media was changed 12 hours after transfection and viral-containing supernatant was collected 24 and 48 hours later ...
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bioRxiv - Immunology 2021Quote: ... The cells were transiently transfected with polyethylenimine (Polysciences) and respective vector plasmids in Opti-MEM and FreeStyle 293 medium (Gibco ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected using PEI Max (Polysciences) according to the manufacturer’s protocol ...