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Citations for Polysciences :
601 - 650 of 730 citations for 20 Hydroxyecdysone ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Flp-In Trex cells were co-transfected with a 1:9 ratio (w/w) of GFP vector:pOG44 Flp recombinase vector using PEI Max 40k (Polysciences). To select for integrant cells ...
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bioRxiv - Bioengineering 2023Quote: ... isolated with ultracentrifugation were resuspended and incubated in 2.5% glutaraldehyde for 1 hour at room temperature and loaded on plasma cleaned carbon-coated copper grid (Polysciences). Fixed MBV samples were incubated on the grids for 20 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Animals were immobilized on a pad composed of 4% ultrapure agarose in H2O in 1-2 µL of 5 mM levamisole in H2O or in a suspension of 0.1 µm polystyrene beads (Polysciences) in H2O ...
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bioRxiv - Neuroscience 2023Quote: ... followed by brief nuclear counterstaining using Hoechst (1:10,000) in PBS and mounting on a glass slide with Aqua-Poly-Mount (Polysciences).
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bioRxiv - Biochemistry 2023Quote: ... cells were transfected with a total of 125 μg DNA encoding the full ECD per 250 mL of cells using polyethylenimine (PEI, 1:3 DNA:PEI ratio; Polysciences), according to the manufacturer’s protocol ...
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: ... packaging vector psPAX2 and envelope vector pMD2.G with a ratio of 4:2:1 using PEI Max (Polysciences) according to standard protocols with a 3:1 PEI Max:DNA ratio for 6 to 9 h ...
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bioRxiv - Neuroscience 2024Quote: ... Cells at ∼80% confluency were transfected with DNA plasmids using polyethyleneimine max according to the manufacturer’s manual (PEI Max; 24765-1, Polysciences). For CaST experiments ...
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bioRxiv - Neuroscience 2024Quote: ... the left sides of the brains were placed (anterior portion facing downwards) in disposable cryomold (Polysciences Inc. #18986– 1), and frozen in O.C.T ...
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bioRxiv - Neuroscience 2024Quote: ... the transfer and pDGM6 packaging plasmids were co-transfected in 293T cells (ATCC) using PEI reagent (Polysciences, 23966-1), as described previously 52 ...
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bioRxiv - Microbiology 2024Quote: ... Transient DNA transfections were performed using linear poly(ethylenimine) (1 mg/mL of solution in 10 mM Tris-HCl, pH 6.8; Polysciences), Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then transfected with 2 µg of GFP-tagged construct and 2 µg of mCherry using a 3:1 ratio of PEI MAX (Polysciences) in Opti-MEM (Thermo Fisher) ...
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bioRxiv - Cell Biology 2020Quote: ... MLV-GagPol/pHIV 8.1 and pHIT VSVg at a ratio of 2:3:1 for 48 h using polyethylenimine (Polysciences, Germany). 293T supernatant was collected and filtered through a 0.2-μm filter before being used to transduce MDCK II cells ...
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bioRxiv - Molecular Biology 2021Quote: Lentivirus expressing short-hairpin RNAs was prepared by transfecting PLKO.1 shRNA plasmids and packaging plasmids containing psPAX2 and pMD2.G into 293T cells using PEI (Polysciences). Collected conditional media containing virus particles were used to transduce cells in the growth media supplemented with Polybrene for 24 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected with 500 ng of reporter and miRNA-vector using 4 μg polyethylenimine (PEI) (Polysciences Inc, 24765-1). Dual-luciferase assays were carried out using the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... followed by ∼3 microliters of the final assay solution containing 5 ng mL-1 500 nm diameter beads (PolySciences Inc,), coated nonspecifically with neutravidin18(ThermoFisher Inc.).
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bioRxiv - Cell Biology 2019Quote: ... were placed within a of droplet of 1 µl M9 buffer containing 1mM levamisole and 0.1 µm polystyrene microbeads (Polysciences, USA) on a 10 % agarose pad ...
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bioRxiv - Cell Biology 2019Quote: Age-synchronized males (3 d after bleaching adult hermaphrodites fertilized by males) were placed in droplets of 1 µl polystyrene microbeads solution (bead diameter of 0.1 µm; Polysciences, USA) on 10% agarose pads ...
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bioRxiv - Microbiology 2021Quote: ... expression medium was added to reduce cell density to 1.0 × 106 ml−1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Microbiology 2021Quote: ... the construct was expressed in 293F by transfecting with PEIMAX PEI MAX™ (24765-1, Polysciences, Inc., Warrington, PA, USA) according to the manual ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transiently transfected at a density of 3 × 10^6 cells/mL using linear polyethylenimine (Polysciences Cat# 23966-1). The media was supplemented 24 hours after transfection with 2.2 mM valproic acid ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293T cells were co-transfected with pAS-Pyl-AF and an amber codon-containing EMC construct at a ratio of 4:1 using PEI “MAX” (Polysciences) and were grown for 2 days in the presence of 0.5 mM AbK (Iris Biotech) ...
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bioRxiv - Biophysics 2021Quote: ... were co-transfected with plasmids containing the SOSIP sequence and furin protease in a 3:1 ratio using PEI MAX (Polysciences). Six to seven days post-transfection the supernatant was cleared by filtration and the SOSIP purified by Galanthus nivalis lectin affinity ...
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bioRxiv - Neuroscience 2020Quote: ... The homogenized mixture was then poured into a solution containing 1% polyvinyl alcohol (25 kDa, 88% hydrolys, Polysciences, Warrington, PA) in water under continuous stirring ...
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bioRxiv - Cell Biology 2020Quote: HEK293T stable cell lines for HSPB8-V5 were transiently transfected with different wild type or mutant BAG3-GFP constructs using PEI (23966-1, PolySciences Europe ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 50 to 100 million J-Lat 11.1 cells per condition were collected and crosslinked with 1% formaldehyde (Polysciences inc.) in 40 ml PBS ...
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bioRxiv - Neuroscience 2022Quote: ... the cells were transfected as follows: 100 μL of 1 mg/mL polyethylenimine (PEI; Polysciences, 23966-2, linear 25 kDa) was added to 2 mL of DMEM and incubated for 10 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were incubated for 2h at RT and then washed in PBS and further incubated for 30 minutes in 1 ml 50 mM ethanolamine (Polysciences). The beads were then washed 3 times in PBS and left in PBS on a cold room rotator.
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bioRxiv - Pathology 2021Quote: ... then 1:2 mixture of propylene oxide and Polybed 812 epoxy resin for 4 hours and infiltrated overnight in 100% resin (08792-1, Polysciences). Samples were transferred to embedding molds and polymerized at 60□ overnight ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... HEK 293 cells in a single well of 6-well plate (confluency ≥80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Biochemistry 2021Quote: ... SARS-CoV-2 S trimer expression vector was transiently transfected into Expi293TM cells using 1 mg/mL of polyethylenimine (Polysciences). Five days post transfection ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... 5 μg of an HIV-1 Gag-Pol expression plasmid (pCRV1/GagPol) and 1 μg of VSV-G expression plasmid in 293T cells in 10-cm dishes using polyethylenimine (PolySciences). Virus-containing supernatant was collected and filtered (0.2μm ...
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bioRxiv - Genetics 2022Quote: Circulating blood monocytes were labeled in vivo by retroorbital intravenous injection of 1 μm Flouresbrite YG microspheres (Polysciences Inc, PA) diluted 1:4 in sterile PBS in mice undergoing regression 3 days prior to harvest ...
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bioRxiv - Microbiology 2019Quote: ... expression medium was added to reduce cell density to 1.0×106 ml-1 for transfection with polyethyleneimine (PEI) (Polysciences, Inc). Next ...
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bioRxiv - Immunology 2020Quote: ... the cells were transfected using 6 μg p509 and 6 μg p2670 plasmid DNAs (Neuhierl et al., 2002) mixed with 72 μl of a 1 mg/ml stock solution of polyethylenimine (#24765; Polysciences) in water (pH 7.0 ...
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bioRxiv - Microbiology 2020Quote: ... The cells were incubated at 37°C for 72 hours after which cells were fixed with 1 ml of 10% formaldehyde (Polysciences) was added for 24 hours to ensure inactivation of virus ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: HEK 293 cells in a single well of 6-well plate (confluency ≥ 80%) were transfected with 2 μg of DNA using polyethyleneimine (PEI, 1 mg/ml, MW = 25,000 g/mol) (Polysciences Inc) at a DNA:PEI ratio of 1:6 (w/v) ...
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bioRxiv - Molecular Biology 2020Quote: ... Yeast strains were grown in rich medium (YPD) to mid-log phase (∼1.5×107/ml) and cross-linked with methanol-free formaldehyde (1% final concentration, Polysciences Inc) for 30 min and quenched with 0.25M final concentration of glycine (from 2.5M stock ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid DNA (1 mg) was mixed by dropwise addition of 2 mg linear polyethylenimine MW 25000 (Polysciences Inc, Warrington USA) in 25 ml Gibco Hybridoma-SFM (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... 293F cells were transfected at a density of 106 cells/mL in Freestyle 293 media using 1 mg/mL PEI Max (Polysciences). Transfected cells were cultured for 7 days with gentle shaking at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Four hundred μl of undiluted culture was then mixed with 50 μl of a 1:100 dilution of 1.75 μm fluorescent microspheres (Polysciences 19392). Four hundred fifty μl of the bead/culture suspension was added to the epithelial surface of individual tissue sections ...
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bioRxiv - Immunology 2020Quote: HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
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bioRxiv - Immunology 2021Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45 μM filter and stored at −70 °C.
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses were produced by co-transfection with a lentiviral backbone (HIV-1 pNL4.luc encoding the firefly luciferase gene) and either of the SARS-CoV-2 spike plasmids with PEIMAX (Polysciences). Culture supernatants were clarified of cells by a 0.45-μM filter and stored at −80 °C ...
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bioRxiv - Microbiology 2022Quote: ... HEK293T cells were transfected with the pNL4-3-inGluc and S constructs in a 2:1 ratio using polyethyleneimine transfection (Transporter 5 Transfection Reagent, Polysciences) in order to generate viral particles ...
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bioRxiv - Immunology 2022Quote: The HEK293T cell line was then transfected with both the pYD7-H57Fab-HC-PIF plasmid and the pTT22-H57Fab-LC plasmid (ratio 1:3) using polyethylenimine (PEI, Polysciences). Cells were then maintained in DMEM supplemented with 2% FBS ...
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bioRxiv - Biochemistry 2023Quote: ... pCAG-FIP200NTD (1-640) was co-transfected with pCAG-TSF-ATG13363-517-ULK1MIT (836-1050) using the polyethylenimine (PEI) (Polysciences) transfection system ...
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bioRxiv - Bioengineering 2024Quote: ... AORBs were then transferred to optimal cutting temperature (Tissue-Tek, OCT) compound (Sakura, 4583) in a cryomold (Polysciences, 18986-1), with as little PBS as possible ...
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bioRxiv - Neuroscience 2024Quote: ... helper plasmid in a 1:4:2 ratio by weight for a total of 40 µg mixed with PEI (40K MW #24765 Polysciences) in DPBS (Invitrogen 14190144) ...
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bioRxiv - Immunology 2023Quote: ... Expi293 cells were used for transient transfection with the suitable S2P stabilized S-expression plasmids or other vectors by using 1 mg/mL polyethylenimine (PEI, Polysciences). The supernatant was harvested and the S trimer was purified using Ni-NTA resin (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... NIH)67,70 and a plasmid encoding the full Env gp160 in a 3:1 ratio in HEK293T cells (ATCC) using the PEIMAX transfection reagent (Polysciences). Following 48 hours ...