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Citations for ApexBio :
1 - 50 of 52 citations for Prothymosin alpha Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... FLAG peptide (ApexBio, A6002) was added to lysates to a concentration of 1 µg/ml to reduce non-specific interactions ...
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bioRxiv - Biochemistry 2021Quote: ... with similar buffers but 3xFLAG peptide replaced with HA peptide (A6004, APExBIO, Houston, TX) for elution ...
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bioRxiv - Biochemistry 2019Quote: ... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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bioRxiv - Cell Biology 2022Quote: ... RIPA buffer containing FLAG peptide (ApexBio) by incubating on ice for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... 0.4 mg/mL 3xFLAG peptide (APExBIO)).
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bioRxiv - Immunology 2022Quote: ... containing 160µg/mL 1xFLAG peptide (ApexBio) pH 7.5 following a 1-hour incubation with mild agitation ...
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bioRxiv - Biochemistry 2019Quote: ... and 0.5 mg/ml 3xFLAG peptide (APExBIO). To determine concentration and purity ...
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bioRxiv - Biochemistry 2019Quote: ... 250 μg/mL 3x FLAG peptide (APExBIO). The eluted fraction was concentrated using a centrifugal filter unit (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1 μg/μl 3x FLAG peptide (ApexBio) at 37 °C for 15 min with shaking at 1,000 RPM The two elution fractions were pooled and samples were prepared with 4x LDS sample buffer and 10× sample-reducing agent (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... and eluted with 3x FLAG peptide (APExBIO, USA). Following concentration by centrifugation (Amicon Ultra ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.5% blocking reagent (Cat No. K1052; APExBIO, Houston, TX) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... and captured complexes were eluted with 3xFLAG-peptide (ApexBio). Those complexes were further enriched through a second round of selection ...
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bioRxiv - Immunology 2023Quote: ... and eluted with 160 μg/mL FLAG peptide (APExBIO) in Tris buffered saline followed by streptavidin resin (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for 30min with a Flag peptide (ApexBio A6001) and later boiled/loaded into SDS-PAGE gel.
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Following elution for 1.5 h with 0.3 mg/ml 3xFlag peptide (ApexBio), eluted proteins were cleaved with TEV protease (0.25 mg/ml final concentration ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by elution with 250 ng/μL 3 × FLAG peptide (ApexBio, A6001). Purity and concentration were verified by PAGE ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Microbiology 2021Quote: ... FLAG and GFP IPs were eluted with 3XFLAG Peptide (ApexBio, cat# A6001), then separated by SDS-PAGE and processed for immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 3 times wash with 200ng/μL 3x FLAG peptides (APExBio) for elution ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG elutions were performed two times with the 250μg/ml 3XFLAG peptide (ApexBio) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Immunology 2022Quote: ... 0.005% LMNG with 0.0005% CHS and 100 mg/ml 3xFLAG peptide (ApexBio, Catalog number A6001).
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bioRxiv - Molecular Biology 2022Quote: ... Beads were then incubated in 40 µL of 100 ng/mL 3x FLAG peptide (APExBIO) at 211 g for 1 hour at room temperature (Eppendorf ThermoMixerC) ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
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bioRxiv - Biophysics 2023Quote: ... and the complex was eluted with 5 CVs of 3×Flag peptide (0.1 mg/ml; ApexBio). The eluted protein was further purified by gel filtration using a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... immunoprecipitated complexes were eluted with 150 μg/mL of 3 × FLAG peptide (ApexBio, Houston, TX, USA) and subjected to Western blotting.
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bioRxiv - Biophysics 2024Quote: ... The bead-bound remodeler complex was eluted twice using 0.5 mg/mL 3x Flag peptide (APExBIO), with the first elution incubated for at least 2 h ...
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bioRxiv - Biophysics 2020Quote: ... Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio, Houston, TX). Myosin purity was assessed by Coomassie-stained SDS-polyacrylamide gels and protein concentration was determined by measuring the absorbance at 280 nm using extinction coefficient ε280nm = 93,170 M-1cm-1 ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio, Houston, TX). Myosin purity was assessed by coomassie-stained SDS-polyacrylamide gels (Figure S2 ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
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bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... followed by elution with 50 μL of 1 mg/mL 3x-Flag (DYKDDDDK) peptide (ApexBio Technology; A6001) per 100 mL of initial culture volume ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-TOE1 was eluted with 100 µl of NET-2 containing 150 ng/µl FLAG peptide (ApexBio) and 10% glycerol by rotating at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
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bioRxiv - Cell Biology 2021Quote: ... Resin was washed again with 3 bed volumes of buffer and then eluted in 5×0.5 bed volumes of buffer containing 0.25 mg/mL 3×FLAG peptide (APExBio). The protein was further purified by gel filtration (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were washed with lysis buffer and Flag-tagged GRASP55 and GRASP65 were eluted with 0.5 mg/ml 3xFLAG peptide (Apexbio) in lysis buffer ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
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bioRxiv - Bioengineering 2019Quote: Suppression of spontaneous calcium release was done by blocking the forward mode of the NCX using SEA0400 (ApexBio, cat no. A3811) at 2μM and at 4μM and by blocking the RyR receptors using Ryanodine (Tocris ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-importin α purified from mitotic and interphase HeLa cells was eluted from the beads using 300 µg/ml 3xFLAG-tag peptide (Apexbio). The 58 kDa bands were cut from the gel and processed by the Proteomics Core Facility at UT Southwestern.
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... For 3X FLAG immunoprecipitations elutions were performed with 50 µL of lysis buffer supplemented with 1 mg/mL 3X FLAG peptide (ApexBio).
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The GABAARs were eluted with aliquots of 200 μg/ml FLAG tag peptide and 100 μg/ml 3X FLAG (ApexBio) in the washing buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the M2 gel was washed with wash buffer (cell lysis buffer with 300 mM NaCl) for three times and then eluted with 3X Flag peptide (ApexBio). The eluate were mixed with 4 X SDS loading buffer and boiled ...
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bioRxiv - Cancer Biology 2019Quote: ... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: ... Protein was eluted twice from the resin by incubating with 300 µL of 500 µg/mL 3X Flag Peptide (APExBIO, A6001) rotating at 4 °C for 20 minutes ...