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Citations for ApexBio :
1 - 50 of 63 citations for Prestained Protein Peptide Standards since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... bound proteins were eluted with 3xFLAG peptide (0.5 mg/ml, ApexBio). Elution was performed 2 times with 0.1 ml 3xFLAG peptide for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... the bound proteins were eluted with 3xflag peptide (ApexBio, Houston, TX, USA) for 1 h at 4°C.
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bioRxiv - Immunology 2021Quote: ... The protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001), incubated with an excess of NEK7 for 1h on ice and further purified by size exclusion chromatography using Superose 6 10/300 GL column (GE Healthcare ...
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bioRxiv - Molecular Biology 2021Quote: ... FLAG proteins were eluted for 10-20 mins with 250 ug/ml 3× FLAG peptide (APExBIO) in Isotonic Wash Buffer at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The protein was eluted with buffer A supplemented with 0.2 mg/ml 3× FLAG peptide (APExBio A6001), concentrated in a 30-kD MWCO Amicon centrifugal filtration device ...
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bioRxiv - Biochemistry 2022Quote: ... and the proteins were eluted by incubating the beads with 200 μg/mL 3xFLAG peptide (APExBIO, A6001) for 2 hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted by incubation with buffer A containing 0.5 mg/ml 3xFLAG peptide (ApexBio, Houston TX) at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The protein was eluted with buffer A supplemented with 0.25 mg/ml 3× FLAG peptide (A6001; APExBio) for biochemical assays ...
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bioRxiv - Molecular Biology 2022Quote: ... FLAG peptide (ApexBio, A6002) was added to lysates to a concentration of 1 µg/ml to reduce non-specific interactions ...
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bioRxiv - Biophysics 2024Quote: ... The protein was eluted with 5 CV of purification buffer supplemented with 150 μg/mL 3X-FLAG peptide (Apexbio). Fractions containing NavPas were pooled and concentrated ...
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bioRxiv - Biochemistry 2021Quote: ... with similar buffers but 3xFLAG peptide replaced with HA peptide (A6004, APExBIO, Houston, TX) for elution ...
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bioRxiv - Cancer Biology 2019Quote: ... Proteins were eluted using 2 50 μL washes of PBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). CuAAC was performed to append rhodamine-azide onto alkyne probe-labeled proteins and after addition of loading buffer samples were separated on precast 4-20% TGX gels (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... and rotated at 4° C for 2 h before washing 3 × with 500 μL PBS and subsequent elution of FLAG-tagged proteins with 100 μL of 250 ng/μL 3 × FLAG peptide (APExBIO) in PBS ...
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bioRxiv - Biochemistry 2019Quote: ... 3XFlag peptide was from APExBIO. Anti-Flag(M2 ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: ... Protein was eluted twice from the resin by incubating with 300 µL of 500 µg/mL 3X Flag Peptide (APExBIO, A6001) rotating at 4 °C for 20 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... were performed before proteins were eluted using 50 μL of TBS supplemented with 250 ng/μL 3 × FLAG peptide (ApexBio A6001). Supernatant (30 μL ...
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bioRxiv - Cell Biology 2022Quote: ... RIPA buffer containing FLAG peptide (ApexBio) by incubating on ice for 5 min ...
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bioRxiv - Biochemistry 2021Quote: ... 0.4 mg/mL 3xFLAG peptide (APExBIO)).
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bioRxiv - Immunology 2022Quote: ... containing 160µg/mL 1xFLAG peptide (ApexBio) pH 7.5 following a 1-hour incubation with mild agitation ...
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bioRxiv - Immunology 2021Quote: ... The beads were washed with 50 column volumes (CV) of the same buffer and the protein was eluted with 100 μg/ml 3xFLAG peptide (ApexBio, Cat. no: A6001). The eluted protein was incubated with 0.5 mM ADP and 0.5 mM MCC950 (Millipore Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... and 0.5 mg/ml 3xFLAG peptide (APExBIO). To determine concentration and purity ...
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bioRxiv - Biochemistry 2019Quote: ... 250 μg/mL 3x FLAG peptide (APExBIO). The eluted fraction was concentrated using a centrifugal filter unit (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... or 1 μg/μl 3x FLAG peptide (ApexBio) at 37 °C for 15 min with shaking at 1,000 RPM The two elution fractions were pooled and samples were prepared with 4x LDS sample buffer and 10× sample-reducing agent (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2023Quote: ... and eluted with 3x FLAG peptide (APExBIO, USA). Following concentration by centrifugation (Amicon Ultra ...
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bioRxiv - Molecular Biology 2020Quote: ... and captured complexes were eluted with 3xFLAG-peptide (ApexBio). Those complexes were further enriched through a second round of selection ...
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bioRxiv - Immunology 2023Quote: ... and eluted with 160 μg/mL FLAG peptide (APExBIO) in Tris buffered saline followed by streptavidin resin (Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... incubated for 30min with a Flag peptide (ApexBio A6001) and later boiled/loaded into SDS-PAGE gel.
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bioRxiv - Biochemistry 2020Quote: ... Following elution for 1.5 h with 0.3 mg/ml 3xFlag peptide (ApexBio), eluted proteins were cleaved with TEV protease (0.25 mg/ml final concentration ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by elution with 250 ng/μL 3 × FLAG peptide (ApexBio, A6001). Purity and concentration were verified by PAGE ...
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bioRxiv - Microbiology 2021Quote: ... FLAG and GFP IPs were eluted with 3XFLAG Peptide (ApexBio, cat# A6001), then separated by SDS-PAGE and processed for immunoblotting ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 3 times wash with 200ng/μL 3x FLAG peptides (APExBio) for elution ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG elutions were performed two times with the 250μg/ml 3XFLAG peptide (ApexBio) for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 0.005% LMNG with 0.0005% CHS and 100 mg/ml 3xFLAG peptide (ApexBio, Catalog number A6001).
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bioRxiv - Molecular Biology 2022Quote: ... Beads were then incubated in 40 µL of 100 ng/mL 3x FLAG peptide (APExBIO) at 211 g for 1 hour at room temperature (Eppendorf ThermoMixerC) ...
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bioRxiv - Microbiology 2024Quote: ... Huperzine standards were purchased from commercial vendors: Huperzine A (ApexBio Technology LLC, Houston, TX, USA) and Huperzine B (MilliporeSigma ...
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bioRxiv - Biophysics 2023Quote: ... and the complex was eluted with 5 CVs of 3×Flag peptide (0.1 mg/ml; ApexBio). The eluted protein was further purified by gel filtration using a Superose 6 Increase 10/300 GL column (Cytiva ...
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bioRxiv - Cancer Biology 2023Quote: ... immunoprecipitated complexes were eluted with 150 μg/mL of 3 × FLAG peptide (ApexBio, Houston, TX, USA) and subjected to Western blotting.
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bioRxiv - Biophysics 2024Quote: ... The bead-bound remodeler complex was eluted twice using 0.5 mg/mL 3x Flag peptide (APExBIO), with the first elution incubated for at least 2 h ...
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bioRxiv - Biophysics 2020Quote: ... Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio, Houston, TX). Myosin purity was assessed by Coomassie-stained SDS-polyacrylamide gels and protein concentration was determined by measuring the absorbance at 280 nm using extinction coefficient ε280nm = 93,170 M-1cm-1 ...
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bioRxiv - Biochemistry 2021Quote: ... Beads were washed and myosin was eluted from the beads by 3x FLAG peptide (ApexBio, Houston, TX). Myosin purity was assessed by coomassie-stained SDS-polyacrylamide gels (Figure S2 ...
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bioRxiv - Cell Biology 2023Quote: ... followed by elution with 50 μL of 1 mg/mL 3x-Flag (DYKDDDDK) peptide (ApexBio Technology; A6001) per 100 mL of initial culture volume ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-TOE1 was eluted with 100 µl of NET-2 containing 150 ng/µl FLAG peptide (ApexBio) and 10% glycerol by rotating at 4°C for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Resin was washed again with 3 bed volumes of buffer and then eluted in 5×0.5 bed volumes of buffer containing 0.25 mg/mL 3×FLAG peptide (APExBio). The protein was further purified by gel filtration (Superdex 200 10/300 GL ...
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bioRxiv - Biochemistry 2024Quote: ... pH 6.5 prior to competitive elution with ∼2.5 column volumes of buffer containing 0.3 mg/mL of DYKDDDK tag peptide (A6002, APExBIO). The eluted protein was concentrated on a 10 kDa MWCO PES membrane Vivaspin 6 Centrifugal Concentrator (Sartoris ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were washed with lysis buffer and Flag-tagged GRASP55 and GRASP65 were eluted with 0.5 mg/ml 3xFLAG peptide (Apexbio) in lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-importin α purified from mitotic and interphase HeLa cells was eluted from the beads using 300 µg/ml 3xFLAG-tag peptide (Apexbio). The 58 kDa bands were cut from the gel and processed by the Proteomics Core Facility at UT Southwestern.
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bioRxiv - Cell Biology 2021Quote: ... For 3X FLAG immunoprecipitations elutions were performed with 50 µL of lysis buffer supplemented with 1 mg/mL 3X FLAG peptide (ApexBio).
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The resin was resuspended in 800 µL of low salt wash buffer containing 2 mg/mL 3X-FLAG peptide (ApexBio) and incubated for 30 min at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... The GABAARs were eluted with aliquots of 200 μg/ml FLAG tag peptide and 100 μg/ml 3X FLAG (ApexBio) in the washing buffer ...