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Citations for Repligen :
1 - 50 of 66 citations for Centrosomal Protein 63 kDa CEP63 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound nucleosomes were captured using protein A agarose (Repligen) beads ...
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bioRxiv - Microbiology 2022Quote: ... TFF concentration (Repligen N06-E100-05-N, 100 kDa) optiprep (Sigma ...
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bioRxiv - Immunology 2022Quote: A 50 kDa cut-off Tangential flow filtration column (Repligen) was washed and equilibrated as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) using 6-8 kDa dialysis tubing (Repligen) at 4 °C overnight ...
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bioRxiv - Microbiology 2022Quote: ... antibodies were purified using the Protein A resin (Repligen, CA-PRI-0100). All the antibody preparations were reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... The polymer was further purified using dialysis (1 kDa MWCO, Repligen) against 3.5 liters of deionized water with a total of four changes over 72 h at room temperature and lyophilized to obtain a pure white solid powder ...
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bioRxiv - Genetics 2020Quote: ... Vectors that encoded the anti-FAM19A5-IgG1 antibody were transfected into HEK293F cells and the recombinant antibody was purified using Protein A beads (RepliGen). Anti-FAM19A5 antibodies that recognized the epitopes formed at N-terminal and C-terminal regions were generated and called N-A5-Ab and C-A5-Ab ...
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bioRxiv - Biophysics 2021Quote: ... using a 10 kDa MWCO dialysis membrane (Spectrum® Repligen, Waltham, MA, USA). For ACE2 purification ...
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bioRxiv - Biophysics 2019Quote: ... pH 7.4) overnight at 4 C in 3.5 kDa MWCO dialysis tubing (Repligen). 1-1.5 mL of Ni2+ Sepharose Excel (GE Life Sciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody-bound nucleosomes were captured for 1 hr at 4°C using protein A agarose (Repligen) beads ...
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bioRxiv - Bioengineering 2021Quote: ... 8-10 kDa molecular weight cut off (MWCO) was purchased from Repligen (Waltham, MA, USA). TLR agonists (ssRNA40/LyoVec and CpG-A ODN 2216 ...
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bioRxiv - Immunology 2020Quote: ... Formulation was performed by TFF (ProStream mPES,1 m2 10 kDa MWCO, L-screen; Repligen) operated with a TMP of 15 psig at a process flux of 8 LMH ...
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bioRxiv - Immunology 2020Quote: ... Harvest was performed via tangential flow filtration using 750 kDa hollow fiber cartridge (Spectrum/Repligen) at load challenge <50 g/m2 ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was concentrated using a 100 kDa TFF column (Repligen N06-E100-05-N) and viruses were pelleted by ultracentrifugation (141,000 × g ...
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bioRxiv - Bioengineering 2023Quote: ... a 100-kDa MWCO MidiKros mPES membrane (D04-E100-05-N; Repligen, Boston, MA, USA) with 6 diafiltration steps and a transmembrane pressure of 5 PSI was used to concentrate samples to ∼10-15 mL26 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2 mg of anti-MHC-I mouse monoclonal antibodies (W6/32) covalently conjugated to Protein A sepharose (Repligen) using DMP as previously described [42,43] were added to the clarified supernatants and incubated with constant agitation for 2 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... The suspension was then diafiltered using a MicroKros cross-flow diafiltration cartridge (500 kDa cutoff) from Repligen, Rancho Dominguez ...
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bioRxiv - Synthetic Biology 2023Quote: ... and buffer exchanged into PBS by overnight dialysis through regenerated cellulose tubing (6-8 kDa MWCO, Repligen). To test conjugation ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02% sodium azide) overnight at 4C using 6-8 kDa molecular weight cut-off dialysis tubing (Repligen) and the expression tag was simultaneously removed using recombinant TEV protease (∼1:20 ratio mg/mg) ...
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bioRxiv - Bioengineering 2023Quote: ... 12-14 kDa molecular weight cut-off (MWCO) dialysis membrane was acquired from Repligen (Waltham, MA, USA). Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates (500 µg for HEK293 or 2 mg for U251N) were incubated with 3 µg of indicated antibodies and 12 µl protein-A sepharose beads (IPA300, Repligen) (co-IP ...
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bioRxiv - Cell Biology 2022Quote: ... 200 µg for HEK293) were incubated with 3 µg of the indicated antibodies and 12 µL protein sepharose beads (IPA300, Repligen) at 4°C for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol and 2 mM dithiothreitol (DTT)) in 12-14 kDa Spectra/Por dialysis tubing (Repligen, cat#123697) with mild agitation at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.70) using hollow fiber filter modules (100 kDa pore size, modified polyethersulfone; Spectrum MicroKros and MidiKros, Repligen). Liposomes were syringe filtered through 0.45 µm cellulose acetate membranes (VWR) ...
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bioRxiv - Bioengineering 2020Quote: Hollow fiber cartridges (molecular weight cut off, MWCO: 500 kDa, material: polysulfone) were supplied by Repligen (Rancho Dominguez, CA). Amicon® ultra-15 centrifugal filter units (MWCO ...
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bioRxiv - Bioengineering 2022Quote: ... Then the slurry was washed with 10x of 85 mM imidazole in PBS and the active protein was eluted with 250 mM imidazole and dialyzed three times against 100-fold excess of PBS at 4 °C (3,000 kDa molecular weight cut-off, Spectra/Por, Repligen). Finally ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were first purified using CaptivA Protein A affinity resin (Repligen), unless otherwise specified ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dialyzed against 3 liters of Milli-Q water at 4°C using a 1 kDa cut-off dialysis tubing (Repligen Spectra/Por 6 Pre-Wetted Regenerated Cellulose ...
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Immunogenic fusion proteins induce neutralizing SARS-CoV-2 antibodies in the serum and milk of sheepbioRxiv - Bioengineering 2022Quote: ... Supernatants from each culture were dialyzed into 1.5 L 1X PBS for 24 hours using 6-8 kDa molecular weight cut off (MWCO) dialysis tubing (Repligen, #132655). Dialysed samples were collected and imidazole was added to a final concentration of 20 mM ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A beads (Repligen) were used to capture antibody-bound protein at 4 °C for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A beads (Repligen) were blocked in BC150 supplemented with 1% Fish gelatin (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A agarose beads (RepliGen) were blocked for 1 hr at 4°C in Buffer BC150 containing 1% fish skin gelatine (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in 1x TE buffer at 4⁰C for 1 hr ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein A agarose beads (Repligen) were blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA in native ChIP incubation buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A agarose beads (Repligen) were used to capture the immunoprecipitated material for 1 h at 4°C ...
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bioRxiv - Immunology 2021Quote: ... EV-depleted FBS was collected from the filtrate from FBS introduced through tangential flow filtration (TFF) system with a 500 kDa molecular weight cutoff (MWCO) hollow fiber filter (polysulfone, Repligen, Waltham, MA). After 48 h ...
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bioRxiv - Immunology 2022Quote: ... followed by diafiltration and concentration through tangential flow filtration using a 750 kDa molecular weight cut-off (MWCO) modified polyethersulfone (mPES) membrane (Repligen, Waltham, MA). Terminal filtration of the saRNA material was done using a 0.22 µm PES filter ...
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bioRxiv - Physiology 2022Quote: ... and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen). All proteins were further polished with Superdex 200 Increase 10/300 GL (GE Healthcare Life Sciences ...
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bioRxiv - Microbiology 2021Quote: Particles in the flow-through from the filtration stage were concentrated on a 100 kDa tangential flow filter (Spectrumlabs, Repligen, Waltham, Massachusetts, USA) to a final volume of ~500 ml ...
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bioRxiv - Microbiology 2024Quote: ... The recombinant proteins were purified from supernatants by NGL COVID-19 Spike Protein Affinity Resin (Repligen) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A agarose (Repligen, #CA-PRI-0100), Mouse anti-HA magnetic beads (Pierce ...
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bioRxiv - Neuroscience 2022Quote: ... protein-A Agarose beads (Repligen 102500-03) and RFP trap magnetic beads (Chromo Tech rtma ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared conditioned tangential flow media was placed onto a 30 kDa cut-off filtration (TFF) column (Repligen, S02-E030-05-N, surface area 790 cm2). The media was concentrated 10-fold by placing the feed tube in the media and applying 12 PSI transmembrane pressure (TMP ...
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bioRxiv - Immunology 2021Quote: Protein-A sepharose beads (Repligen, Waltham, Mass. USA) were covalently conjugated to 10 mg/mL W6/32 (pan-anti-HLA-I ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein A agarose beads (Repligen, 10-1003-02) or protein A/G magnetic beads (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... Protein A-Sepharose was from Repligen (Needham, MA). Other common reagents were from Sigma-Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... 60 μL of Protein A agarose bead slurry (Repligen) were washed and resuspended in lysis buffer ...
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bioRxiv - Neuroscience 2022Quote: ... 500 μg of total brain or heart lysate extracts were incubated with 15 μL of Protein-A Sepharose beads (CaptivA protein resin, Repligen, Cat#CA- PRI-0100) and 2 μg of anti-Cav1.2 α1-subunit or control rabbit IgG antibody ...
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bioRxiv - Molecular Biology 2020Quote: Sonicated chromatin was precleared using Protein A agarose beads (Repligen) for 1 hour (1h ...
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bioRxiv - Molecular Biology 2019Quote: ... or protein A agarose beads (Repligen, for histone or RNAPII ChIP) blocked with 1 mg/ml BSA and 1 mg/ml yeast tRNA ...