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Citations for ChromoTek :
1 - 50 of 86 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... 50 using RFP-Trap single chain antibodies (Chromotek). Enrichment of specific DNA fragments was validated by qPCR at the ARR7 promoter region24 ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
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bioRxiv - Cell Biology 2019Quote: ... and the soluble fraction was incubated with single chain anti GFP antibody (GFP-binder) (GFP-Trap-M; Chromotek) conjugated to sepharose beads ...
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bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
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bioRxiv - Cell Biology 2020Quote: ... anti-GFP conjugated to Atto488 (1:100; ChromoTek gba-488; Fig. 3B-C, S2A,C), anti-mouse secondary antibodies (1:400 ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Plant Biology 2020Quote: ... or c-Myc rat monoclonal antibody (Chromotek, 9E1-100) at a dilution of 1:1000 followed by goat anti-rat IgG horseradish peroxidase (Abcam ...
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bioRxiv - Plant Biology 2023Quote: ... the chromatin was treated with ultrasound and the DNA fragments were precipitated using GFP-TRAP Magnetic Agarose beads (Chromotek, gtma-20). After protein digestion ...
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bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
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bioRxiv - Biochemistry 2020Quote: ... C GFP-Trap (Figure S3B) or RFP-Trap (Figure S3C) beads (Chromotek) were first coated with the ATG12–ATG5-ATG16L1-GFP protein (Figure S3B ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200 ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-c-myc beads (VF299569, Thermo) or RFP-Trap Magnetic Agarose (Chromotek) were added to the protein extracts and incubated at 4°C for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated at 4°C overnight with GFP-Trap agarose (Chromotek) or Dynabeads (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) beads as described by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) and washed in lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... Complexes were immunoprecipitated for 90 minutes at 4°C with GFP-Trap beads (Chromotek). After five washes in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and the supernatant incubated with 25 μl of equilibrated GFP-Trap resin (ChromoTek) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek) and washed as manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed on a rotating wheel at 4°C overnight with GFP-Trap beads (Chromotek). The beads were then washed 4 times with lysis buffer ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were incubated (2 h, at 4°C) with 20 µl GFP-Trap®_MA (ChromoTek). Beads were then washed 3 times for 10 min with 1 ml of IP buffer and eluted with Laemmli sample buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated at 4°C for 1 h with GFP-Trap Agarose beads (Chromotek) and washed 4 times ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 4 hours at 4°C with agarose-conjugated GFP-Trap™ beads (Chromotek). Beads were washed once with lysis buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates incubated on a nutator at 4°C for two hours with 20μl of GFP-Trap beads (ChromoTek). GFP-Trap complexes were then washed four times in IP buffer and re-suspended in 2× SDS-PAGE sample buffer ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Pathology 2021Quote: ... 1-4 mL of supernatant was incubated at 4°C with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany) overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysate was cleared by centrifugation and incubated for 2h at 4°C with magnetic GFP-Trap agarose (Chromotek). The beads were washed 3 x 10 min with Buffer 3 and were either used for mass spectrometry analysis or the proteins were eluted in 1× SDS buffer with 5 min incubation at 95°C for western blotting ...
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bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 1h at 4 °C before being added to 10 µl magnetic V5 beads (v5tma, Chromotek), pre-equilibrated in buffer K ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 200 μl of GFP-Trap_MA magnetic beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... for 30 min at 4°C and then with GFP-Trap Magnetic Particles M-270 (gtd-20, ChromoTek GmbH) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Protein samples were then incubated for 1.5 h at 4 ⁰C with 25 µL RFP-Trap agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was cleared by centrifugation at 16,000 g for 5 min at 4 °C before it was immobilized on 20 μl GFP-Trap beads (ChromoTek) for 30 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was cleared at 14,000 rpm for 10 min and incubated for 10 min rotating at 4 °C together with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek). Beads were washed four times with GFP pull-down buffer at 2,500 g for 2 min at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Supernatants were centrifuged at 21,000 g at 4 °C for 10 min and then incubated for 10 min at 4 °C with 12.5 μl pre-equilibrated GFP-Trap beads (Chromotek) rotating ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Plant Biology 2024Quote: ... Lysates were cleared by filtering through miracloth followed by centrifugation (6,000 g, 30 min, 4°C) and then incubated with GFP-Trap (Chromotek) for 4h ...