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Citations for ChromoTek :
1 - 49 of 49 citations for Siglec 15 Cynomolgus HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: YFP-ATM was immunoprecipitated from stably transfected HEK293 cells by GFP-TRAP (Chromotek). The immunoprecipitates were suspended in kinase assay buffer containing 2 μCi of [γ-32P] ATP ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Plant Biology 2022Quote: ... 15 μl of GFP-Trap®_A (ChromoTek) was washed three times with RIPA buffer and added into the supernatant ...
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bioRxiv - Neuroscience 2022Quote: ... 15 µl GFP-Trap Magnetic Agarose (ChromoTek GmbH) (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... 15 μL of GFP-Trap agarose beads (Chromotek, Germany) were incubated with 2 mL of crude protein at 4°C for 3 h and collected as described previously (Yang et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 15 µl of GFP-trap beads (Chromotek; gta-20) were equilibrated in lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... (2021) using 15 μl α-GFP-magnetic beads (Chromotek). Anti-GFP antibody (sc9966) ...
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bioRxiv - Cell Biology 2020Quote: ... GFP-ChAM were pull-down using 15 µL GFP-Trap Agarose (Chromotek), pre-washed three times with ice-cold NETN250 buffer and blocked for 3 h at 4°C on a rotating wheel with 500 µL ice-cold NETN250 buffer supplemented with 2 mg/mL bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2019Quote: ... The supernatant was incubated with 15 µl of RFP-trap slurry (Chromotek) for 2 hours at 4°C on a rotary shaker ...
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bioRxiv - Pathology 2021Quote: ... the supernatant was mixed with 15 μL GFP-trap A beads (Chromotek) and incubated at 4°C for 1.5 h ...
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bioRxiv - Molecular Biology 2022Quote: ... protease inhibitors) and incubated with 15 μl of GFP_Trap magnetic beads (Chromotek) for 1h at 4°C with gentle rotation ...
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bioRxiv - Developmental Biology 2023Quote: ... 15 μl of equilibrated magnetic RFP-Trap Agarose beads (ChromoTek, Planegg, Germany) were added ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were added to 15 µl of GFPTrap-magnetic agarose beads (Chromotek) and incubated with end-over-end rotation for 30 minutes at 4 °C to capture specific binding proteins ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Developmental Biology 2021Quote: ... The lysate was then incubated with 15 ml of GFP trap Beads (Chromotek) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... 15 μl of GFP-Trap®A (pre-equilibrated with lysis buffer, Chromotek) was added to 285μl of normalized cell lysate and incubated at 4°C for 2 hours with gentle rocking ...
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bioRxiv - Genetics 2019Quote: ... lysates were incubated with 15 μl of GFP-Trap Agarose beads from Chromotek for 1 h at 4 †C on a rotary wheel ...
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bioRxiv - Cell Biology 2023Quote: ... lysates were incubated with 15 µl of GFP-Trap Agarose beads from Chromotek for 1h at 4 °C on a rotary wheel ...
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bioRxiv - Molecular Biology 2023Quote: ... or GFP/RFP-Trap Magnetic Agarose beads (15 µL bead suspension per reaction, Chromotek). When using endogenous antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... The filtered extracts were incubated with 15 µL GFP-trap agarose beads (ChromoTek, Germany) at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2021Quote: ... μ 20 mg of total protein was incubated with 15 μl GFP-TrapMA beads (Chromotek) at 4 °C for 2 h ...
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bioRxiv - Developmental Biology 2019Quote: ... For immunoprecipitation of GFP-tagged Imp and Bru1 15 μl of GFP-Trap® (Chromotek) were used ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
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bioRxiv - Plant Biology 2021Quote: ... To each 15 ml of protein extract 40 μl of GFP-TRAP AGAROSE BEADS (50 % slurry, ChromoTek) washed in extraction buffer were added and incubated with gentle agitation for 4 h at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared and used for immunoprecipitation with 15 μl GFP-trap or Myc-trap (Chromotek). Beads were washed with lysis buffer and bound material was eluted from beads with elution buffer (50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 15 µl of Myc-Trap Agarose (with anti-Myc alpaca antibody) (Chromotek, cat. No. yta) and incubated for 3 h at 4°C with end-over-end rotation ...
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Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: ... the rest was incubated with 15 μl of GFP-Trap_MA magnetic agarose beads (ChromoTek GmbH, Planegg-Martinsried, Germany), prewashed with lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were cleared by centrifugation at 16000g for 15 minutes and incubated with GFP-Trap agarose beads (Chromotek) overnight at 4 °C ...
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bioRxiv - Genetics 2020Quote: ... Clarified extract was incubated with 15 μl GFP-TRAP-A beads or blocked agarose beads (bab-20) (Chromotek) equilibrated in C Buffer for 2 h at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were cleared by centrifugation at 14000rpm for 15 minutes and incubated with GFP-Trap agarose beads (Chromotek) overnight at 4°C under rotation ...
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bioRxiv - Cell Biology 2024Quote: Equal volumes of the cleared and normalised lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of clarified and normalised cell lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) for overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of the cleared and normalised lysates were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) pre-equilibrated in lysis buffer ...
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 200 μl of GFP-Trap_MA magnetic beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... The homogenate was clarified by centrifugation at 750 g for 15 min at 4°C and the supernatant was incubated with 40 μl of GFP-Trap MA magnetic Beads (ChromoTek) for 4 h on a rotating wheel at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... Lysates were cleared for 15 min at 20000 rcf and incubated with 20 μL pre-equalibrated GFP-trap or Myc-trap beads (ChromoTek) as indicated for 45 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... then mixtures were centrifuged (15 min, 4º, 14000 rcf) and the supernatant (input) was added to washed anti-GFP beads (Chromotek). Beads and lysates were incubated 2-4 h at 4 °C while rotating ...
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bioRxiv - Plant Biology 2020Quote: ... Total proteins were extracted as indicated above and immunoprecipitation was performed with 15 μL of GFP-trap beads (ChromoTek, Germany) or ANTI-FLAG M2 Affinity Agarose Gel (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... 450 μl of nuclear extract was used for each GFP pull down using 15 μl slurry of GFP-Trap agarose beads (Chromotek), performed in triplicate ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris was pelleted at 17,900 rcf for 15 min and the supernatant was bound to α-myc beads (Chromotek) for 4 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: Chromatin samples were thawed on ice and pre-cleared for 15 minutes by rotation in 25 µl of pre-washed binding control magnetic agarose beads (Chromotek). Chromatin was diluted ten-fold in sonication buffer without SDS ...
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bioRxiv - Cell Biology 2024Quote: ... Equal volumes of clarified and normalised cytosolic and nuclear extracts were incubated with 15-20 μl GFP-Trap Agarose (ChromoTek) for overnight at 4 °C with rotation ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2022Quote: ... The rest of the supernatants were incubated for 15 min at 4°C with 10 μl of GFP-Trap® Agarose beads (gta-10; Chromotek) to remove free GFP and spun at 1200 xg for 1 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 mg nuclear or 3 mg cytoplasmic extract per pulldown was incubated in triplicates with 15 μl of a 50% slurry of GFP-trap beads (ChromoTek, gta-20) or control agarose beads (ChromoTek ...