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Citations for ChromoTek :
1 - 50 of 251 citations for SARS CoV 2 Membrane Envelope Fusion Protein His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Microbiology 2022Quote: ... mCherry fusion proteins were captured with RFP-Trap (Chromotek RTA-20) for 3 h at 4° C ...
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bioRxiv - Cancer Biology 2022Quote: ... GFP fusion proteins were immunoprecipitated using GFP-Trap_MA (ChromoTek, Hauppauge, NY), recovered using a magnetic stand ...
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bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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bioRxiv - Microbiology 2019Quote: ... GFP-fusion protein-complexes were immunoprecipitated with 25 µl GFP-Trap (ChromoTek) for 60 min at 4°C under constant rotation ...
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bioRxiv - Biophysics 2022Quote: ... The fusion protein (Lifeact-mScarlet) was extracted using a RFP-Trap (Chromotek, Planegg – Martinsried ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP fusion proteins were immunoprecipitated with GFP-Trap magnetic agarose (GFP-Trap_MA, Chromotek) beads for 2 hours or overnight at 4°C with rotation ...
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bioRxiv - Biochemistry 2021Quote: Extracts containing GFP-fusion proteins were incubated on GFP-Trap agarose beads (Chromotek) for 20 minutes at 4°C on a rotating wheel in presence of Ethidium Bromide ...
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bioRxiv - Microbiology 2023Quote: ... GFP and the fusion proteins were immunoprecipitated by adding GFP-Trap Agarose (ChromoTek) for 5 h at 4 □ ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Biochemistry 2023Quote: ... eGFP-fusion proteins were immunoprecipitated with GFP-trap magnetic agarose beads (GFP-trap_MA Chromotek) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... The membrane was air-dried for 1 h and immunoblotted with a primary antibody against mNeonGreen tag (Chromotek) at room temperature for 1 h followed by a secondary antibody (680nm anti-mouse ...
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bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
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bioRxiv - Cell Biology 2022Quote: eYFP-tagged fusion proteins were immunoprecipitated using anti-GFP magnetic agarose beads (Chromotek, gtma-20). After induction of gene expression of lentiviral transduced KO2 cells with 1 µg/ml doxycycline ...
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bioRxiv - Neuroscience 2022Quote: ... Overexpressed protein was immunoprecipitated from the cell lysate using tag specific nano-trap (SPOT-Trap-MA, Chromotek) and analysed by WB.
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bioRxiv - Cell Biology 2023Quote: ... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
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bioRxiv - Cancer Biology 2022Quote: ... and subjected to immunoprecipitation of RFP/mCherry/dsRed-tagged or GFP-tagged fusion proteins using RFP-trap or GFP-trap matrix (Chromotek), respectively ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
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bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
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bioRxiv - Biophysics 2022Quote: ... SNAP/CLIP-tag monoclonal antibody (6F9, Chromotek), single-domain antibody (nanobody ...
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bioRxiv - Cell Biology 2023Quote: ... Halo-tag (mouse, 28a8 ChromoTek, 1:200), Alexa Fluor 647-direct labeled CETN1 (rabbit ...
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bioRxiv - Plant Biology 2019Quote: ... The protoplasts were collected and the subsequent work was operated following the protocol for immunoprecipitation of Myc-fusion proteins using Myc-Trap®_MA (Chromotek, Germany). Both the input and the IP samples were separated by SDS-polyacrylamide gel electrophoresis ...
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bioRxiv - Microbiology 2024Quote: ... Lysates from HaCaT cells grown on two 150 mm dishes and infected with HSV-2 strain 186 viruses carrying pUL21-mCherry fusion proteins at an MOI of 3 were prepared at 18 hpi and immobilized on RFP-TRAP beads (Chromotek, Rosemont, IL) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... coli purified EGFP (Chromotek, #EGFP-250) as a stock solution ...
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bioRxiv - Molecular Biology 2020Quote: ... rat monoclonal to myc-tag (Chromotek #9e1, 1:500), mouse monoclonal to Alpha Adaptin (Thermo #MA1-064 ...
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bioRxiv - Cell Biology 2020Quote: ... PARP1-Chromobody (RFP-tag) plasmid was obtained from Chromotek (#xcr). All constructs were amplified with a Plasmid Midi Kit (Qiagen) ...
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bioRxiv - Plant Biology 2019Quote: ... S2Lb-GFP proteins were immunoprecipitated for 2 hours using a GFP-trap system (Chromotek) coupled to magnetic beads ...
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bioRxiv - Cell Biology 2022Quote: GFP and RFP-fusion pulldowns using the GFP- and RFP-Trap (ChromoTek) were performed as described63 ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2019Quote: ... or an anti-Myc-tag Nanobody coupled to agarose (Myc-Trap, Chromotek, Hauppauge, NY) for 1 hour at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... falciparum D10) or GFP-TRAP (PfSkp1GFP-epi, PfCul1GFPKI, and GFP control) antibodies (15 µl slurry/2 mg protein; ChromoTek) for 2 hours at 4°C with gentle shaking ...
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bioRxiv - Plant Biology 2024Quote: ... A volume of 2 mL of total protein was mixed with 25 μL of GFP-TRAP agarose beads (50% slurry, ChromoTek) and incubated shaking overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of the protein lysates was incubated for 2 h with 10 µl of either Myc-Trap® or GFP-Trap® beads (Chromotek). To determine the dependence of interactions on RNA ...
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bioRxiv - Cell Biology 2020Quote: ... Ndc80C-7D-GFP and Ndc80C-His were attached to nanobeads using biotinylated anti-GFP nanobody (Chromotek cat No. gtb-250) and biotinylated anti-His antibody (Quiagen cat No ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were probed with rabbit anti-GFP antibody (Chromotek) at 1:1000 ...
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bioRxiv - Microbiology 2019Quote: ... Solubilized membranes were incubated with GFP-Trap_MA beads (Chromotek) for 1 h at 4°C ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... benthamiana leaves were extracted at 1 mg ml−1 in extraction buffer as described14 and immuno-adsorbed by means of their GFP-tags on GFP-Trap agarose beads (ChromoTek). Immunoblots were developed either directly with Streptavidin-alkaline phosphatase conjugate (Roche) ...
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bioRxiv - Plant Biology 2020Quote: ... Small cuts from the infiltrated regions were incubated for one hour in anti-Spot-Tag VHH/Nanobodies conjugated to the organic fluorophore ATTO488 (Chromotek), diluted 1:1000 in 10 mM Tris-HCl solution pH 7.5 ...
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bioRxiv - Developmental Biology 2023Quote: ... The CUT and TAG experiment was done for two biological replicates using antibodies anti-GFP (Chromotek, PABG1-100, 1:50), anti-YFP (Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... the membrane was incubated overnight in 1° Ab (ChromoTek: 32F6) at 1:5,000 dilution at 4°C on rocker ...
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bioRxiv - Molecular Biology 2023Quote: ... transferred to Nitrocellulose membranes and analysed with anti-RFP (Chromotek) and anti-Flag-HRP conjugated (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2019Quote: ... Nitrocellulose membranes were probed with rat anti-GFP (3H9, Chromotek, 1:2,500), PAP (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2023Quote: ... blotted to nitrocellulose membranes and incubated with antibodies specific for GFP (Chromotek, 3h9), RFP (Chromotek ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...