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Citations for ChromoTek :
1 - 50 of 202 citations for Recombinant Yellow Fluorescent Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rat anti-red fluorescent protein (RFP) monoclonal (5F8; ChromoTek GmbH ...
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bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
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bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
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bioRxiv - Plant Biology 2022Quote: ... rat anti-red fluorescent protein (RFP) (5F8, Chromotek, Planegg-Martinsried, Germany) (1:10,000) ...
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bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
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bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant was incubated with 10µL of green fluorescent protein (GFP)- Trap A (Chromotek) bead slurry for 4 hours at 4°C with constant slow rotation followed by washing the bead slurry 5 times with 500µL IP wash buffer at 1,000 x g for 1 minute at 4°C ...
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bioRxiv - Genetics 2019Quote: ... For IP 25 μL of Red Fluorescent Protein (RFP)-Trap Magnetic Agarose (MA) beads (Chromotek) were added and 25 μL of MA beads (Chromotek ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Plant Biology 2023Quote: ... Five hundred microliters of the supernatant was incubated with 10µL of green fluorescent protein (GFP)-Trap A (Chromotek) bead slurry for 3 hours at 4°C with constant end-over-end rotation ...
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bioRxiv - Neuroscience 2020Quote: ... or Alpaca antibody against Green Fluorescent Protein conjugated to Alexa Fluor 488 (also labels EBFP2, Figure 6B, Chromotek, cat# gb2AF488, 1:100 dilution) to enhance the EBFP2 signal in the green color channel along with DAPI (blue to stain nuclei ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent lamin nanobody (lamin chromobody) plasmid was purchased from ChromoTek and transferred into the NcoI-XbaI site of pGEMHE ...
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bioRxiv - Cell Biology 2020Quote: ... fluorescent nuclear actin nanobody (nuclear actin chromobody) plasmid was purchased from ChromoTek and transferred into the HindIII-EcoRI site of pGEMHE ...
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bioRxiv - Cell Biology 2019Quote: ... The mCherry fluorescent signal was enhanced with rat mAb anti-RFP (Chromotek). As secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Cell Biology 2020Quote: ... To enhance signal from fluorescent reporters we used GFP-booster coupled to Atto488 (gba488) and RFP-booster coupled to Atto594 (rba594) from Chromotek. Secondary antibodies used were from Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
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bioRxiv - Microbiology 2019Quote: ... The nanoboosters - single-domain alpaca antibody fragments covalently coupled to fluorescent dyes — GFP-Booster Atto488 and RFP-Booster Atto594 (Chromotek, Martinsried, Germany) — were used to boost eYFP and mCherry fluorescence ...
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bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were purified by GFP-trap (ChromoTek) immunoprecipitation for 1 hour at 4°C ...
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bioRxiv - Systems Biology 2020Quote: ... Protein complexes were immunoprecipitated using GFP-Trap (Chromotek) and washed 3 times with 50mM ammonium bicarbonate after the precipitation to remove detergent ...
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bioRxiv - Systems Biology 2023Quote: ... The proteins were immunoprecipitated using GFP-Trap (Chromotek) according to manufacturer’s protocol (with slight modifications) ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
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bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cell Biology 2021Quote: ... protein lysates were added to RFP-Trap beads (Chromotek) and incubated for one hour at 4°C on a rotating shaker ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
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bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
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bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
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bioRxiv - Cell Biology 2022Quote: ... GFP-tagged proteins were affinity purified by GFP-trap (ChromoTek) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
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bioRxiv - Cell Biology 2022Quote: ... GFP-GABARAP proteins were immunoprecipitated using GFP-TRAP beads (ChromoTek), incubating for 2hrs at 4°C ...
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bioRxiv - Microbiology 2023Quote: GFP-tagged proteins were immunoprecipitated using GFP-TRAP beads (Chromotek) using TNTE buffer (20mM Tris-HCL pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
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bioRxiv - Genetics 2023Quote: Protein complex was purified using GFP-Trap agarose beads (Chromotek) and separated by western blot ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
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bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...