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Citations for ChromoTek :
1 - 50 of 53 citations for Recombinant Human Lectin Galactoside Binding Soluble 3 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2021Quote: ... and binding control agarose beads (Chromotek).
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bioRxiv - Microbiology 2019Quote: ... binding control magnetic agarose beads (Chromotek) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... or binding control beads (Chromotek #bmab-20). The immune complexes were run on SDS-PAGE and analyzed by Western blotting.
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bioRxiv - Immunology 2019Quote: ... or control binding antibody (bmab-20, Chromotek). For tight chromatin fractionation ...
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bioRxiv - Plant Biology 2020Quote: ... Soluble proteins were incubated with 20 μl of GFP-Trap_MA slurry (Chromotek) or anti-Myc magnetic beads (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... These soluble fractions were incubated with GFP-Trap magnetic agarose (ChromoTek, Germany) overnight at 4°C on a rotating wheel ...
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bioRxiv - Cell Biology 2022Quote: ... 10 µl of GFP-binding agarose beads (Chromotek) were incubated with the protein extract for 1 hour at 4 °C in head-over-shoulder rotation ...
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bioRxiv - Plant Biology 2024Quote: ... a negative control (binding control agarose beads, Chromotek) and input control were used ...
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bioRxiv - Microbiology 2021Quote: ... 25 μl of binding control agarose beads (Chromotek; bab20), prepared in dilution buffer (10 mM Tris-Cl pH 7.5 ...
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bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
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bioRxiv - Cancer Biology 2023Quote: ... or with binding control agarose beads (Chromotek #bab-20) using RIPA lysis buffer and according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was precleared with binding control agarose beads (ChromoTek) for 1hr at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
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bioRxiv - Cell Biology 2019Quote: ... and the soluble fraction was incubated with single chain anti GFP antibody (GFP-binder) (GFP-Trap-M; Chromotek) conjugated to sepharose beads ...
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bioRxiv - Biochemistry 2022Quote: Soluble cell fractions were bound to 10 µL per mL of lysate of GFP-Trap® (ChromoTek™) agarose beads ...
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bioRxiv - Cell Biology 2021Quote: ... The soluble proteins were separated from the cell debris by centrifugation and incubated with GFP-Trap agarose beads (Chromotek) at 4°C for 2 h ...
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bioRxiv - Cell Biology 2019Quote: Soluble fractions of cell extracts were immunoprecipitated with an anti-GFP antibody coupled to magnetic beads (GFP-Trap_MA, ChromoTek) or an anti-HA antibody coupled to agarose beads (EZview Red Anti-HA Affinity Gel ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mg of nuclear extract (nuclear-soluble and chromatin-bound fractions were pooled together) was incubated with Agarose GFP traps (Chromotek) or anti-HA Affinity Matrix (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting protein extracts were centrifuged at maximum speed on a table-top centrifuge for 15 minutes at 4 °C and the supernatant (soluble fraction) incubated with 25 μl GFP-trap magnetic beads (ChromoTek, gtma) for four hours at 4 °C under constant rotation ...
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bioRxiv - Biochemistry 2020Quote: ... Diluted lysate was pre-cleared by incubation with binding control agarose beads (bab-20, CHROMOTEK), at a volume of slurry equivalent to 1:200 (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were incubated for 1 hr with 20 μl of agarose binding control beads (ChromoTek), equilibrated in IP buffer 2 (200 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were centrifuged for 10 min at 20,000 g and equal amounts of supernatant (NP40-soluble fraction only) was loaded on GFP-Trap beads (gta-20, Chromotek, Martinsried, Germany). Beads were incubated with the protein lysate for 1 h at 4 °C and washed three times with wash buffer [20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 30 μl GFP-Trap Agarose or Binding Control Agarose Beads (Chromotek, gta-10 and bab-20) were washed once in dilution buffer (10mM tricHCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... and the supernatant was precleared in 20 μl binding control magnetic beads (bmp-20, ChromoTek; Munich, DE) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: IPs with GFP-tagged proteins were performed with GFP-binding magnetic agarose beads (GFPtrap MA, Chromotek, #gtma-20). Per IP sample ...
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bioRxiv - Genetics 2023Quote: ... Each sample was incubated for preclearing with 200 μL slurry of binding control magnetic agarose beads (ChromoTek bmab) for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2019Quote: ... Pre-clearing of cell lysates was done by adding 50 µl pre-equilibrated binding control magnetic agarose beads (Chromotek) and incubated for 30 min at 4°C on a rotating platform ...
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bioRxiv - Plant Biology 2022Quote: ... The ribosylated NbFBN1a2A-GFP and NbFBN1a-GFP were detected by the anti-pan-ADP-ribose binding reagent after they were pulled down with Co-IP by GFP-Trap_A (ChromoTek).
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bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
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bioRxiv - Plant Biology 2021Quote: ... thaliana plants were incubated with 50-100 μl previously washed 50% GFP-binding protein beads slurry (Chromotek, GFP-Trap®) at 4 °C for 3 hours on a roller mixer ...
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bioRxiv - Microbiology 2022Quote: ... FLAG-KAT7 was incubated with either HIS6-LphD or GST-RomA (HIS6-MBP or GST-MLY1 as binding controls) and the pulldown was performed using DYKDDDDK Fab-Trap beads (Chromotek) followed by western blot analysis.
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bioRxiv - Neuroscience 2023Quote: Chromatin samples were thawed on ice and pre-cleared for 15 minutes by rotation in 25 µl of pre-washed binding control magnetic agarose beads (Chromotek). Chromatin was diluted ten-fold in sonication buffer without SDS ...
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bioRxiv - Developmental Biology 2024Quote: ... To avoid unspecific protein binding the worm lysate was pre-cleared by incubation to uncoupled agarose beads (Chromotek, bab-20) for 1 hour at 4°C ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
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bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
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bioRxiv - Microbiology 2021Quote: ... The resin was then washed 3 times in GFP-Trap_A Wash buffer (Chromotek) and GFP-proteins were eluted using SDS sample buffer at 95°C for 10 min and analyzed by immunoblotting.
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bioRxiv - Immunology 2023Quote: The coding sequence of the Dnmt1-chromobody targeting the human Dnmt1 protein [37] (Uniprot : P26358 · DNMT1_HUMAN) and lamin-chromobody (ChromoTek) targeting the lamin D0 from Drosophila monogaster (Uniprot ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... MEFs were washed extensively and GFP-booster (tagged with Alexa488, ChromoTek, gb2AF488-50, 1:500 in 3%BSA/PBS) and anti-rabbit-Alexa568 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-Trap agarose beads were used to pull down the GFP-human TDP-43 (residues 216-414) or GFP protein (Chromotek, gta-10). Blots were probed with antibodies for mouse anti-Myc antibody (1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were blocked in 3% BSA in PBS-T for 30 min before incubation with GFP-Booster _Atto488 (1:300; Chromotek), or mouse anti-G3BP1 in 3% BSA in PBS-T for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitation was performed using 3 mL of plant extract mixed with 25 µL of GFP-trap magnetic beads (Chromotek) at 4 °C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The extracts were cleared by 10 min centrifugation at 12,000 rpm and the supernatant was incubated on a rotor wheel at 4°C for 3 h with 25 µl of GFP-Trap magnetic agarose bead slurry (Chromotek), pre-washed with extraction buffer ...
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bioRxiv - Microbiology 2021Quote: ... Slides were subsequently blocked overnight in PBS containing 3% BSA before labelling with anti-mNeonGreen mouse monoclonal antibody (32F6; 1:300; Chromotek, Germany) followed by Alexa Fluor 488 goat anti-mouse antibody (1:1000 ...