Labshake search
Citations for ChromoTek :
1 - 50 of 215 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... recombinant binding protein (gt-250, Chromotek). The secondary antibodies we used were AffiniPure goat anti-mouse IgG (H+L ...
-
bioRxiv - Biophysics 2023Quote: ... Purified recombinant Enhanced Green Fluorescent Protein (EGFP) was purchased from Chromotek. Bafilomycin A1 was purchased from InvivoGen ...
-
bioRxiv - Neuroscience 2023Quote: ... recombinant proteins were isolated from the culture medium by incubating anti-GFP nanobody-conjugated agarose (Chromotek). In some experiments ...
-
bioRxiv - Neuroscience 2022Quote: ... Fluorescent signal from the virus was amplified by immunostaining against Red Fluorescent Protein (RFP) (catalog no.5F8, Chromotek GmbH, Germany), followed by secondary antibody-staining with Alexa 546-tagged Goat Anti-rat IgG (catalog no ...
-
bioRxiv - Synthetic Biology 2021Quote: ... FITC MEF values were converted to equivalent fluorescent protein concentrations using calibration curves made using recombinant eGFP from ChromoTek GmbH (reference EGFP-250 ...
-
bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
-
bioRxiv - Neuroscience 2021Quote: ... Recombinant myc and myc-BDNF were from Chromotek and Cusabio ...
-
bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
-
bioRxiv - Immunology 2022Quote: ... and c-Myc–tagged recombinant mouse NEU3 was purified using a Myc-Trap agarose kit (ytak-20; Chromotek, Hauppauge, NY) following the manufacturer’s protocol ...
-
bioRxiv - Pathology 2021Quote: ... sections were blocked and antibodies were incubated at 4°C overnight which was either primarily conjugated with Alexa Fluor 488-conjugated dual monoclonal recombinant alpaca anti-rabbit IgG nano secondaries (Chromotek) or detected with the Vectastain Elite ABC-HRP Kit (VectorLaboratories ...
-
bioRxiv - Microbiology 2022Quote: GFP-tagged target proteins and interacting proteins were affinity purified using GFP-trap agarose beads (Chromotek). Proteins were processed ...
-
bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were determined and an equal amount of protein was mixed with TurboGFP-Trap Magnetic Agarose (Chromotek) for 1 hour at RT with agitation ...
-
bioRxiv - Plant Biology 2021Quote: ... proteins were detected using α-HA (Chromotek) and α-GFP antibodies (Santa Cruz Biotechnology ...
-
bioRxiv - Neuroscience 2022Quote: ... 200 g for lysate was added to 25 µL of HaloTrap beads (Chromotek, #ota-10), prewashed with 500 µL of 10 mM Tris-HCl (pH 7.5) ...
-
bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
-
bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
-
bioRxiv - Neuroscience 2019Quote: ... and 100 ng/ml GFP-Trap Protein (ChromoTek). Tissue samples were homogenized three times at 250 rpm and ten times at 750 rpm on a variable-speed homogenizer (Glas-Col ...
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were purified by GFP-trap (ChromoTek) immunoprecipitation for 1 hour at 4°C ...
-
bioRxiv - Systems Biology 2020Quote: ... Protein complexes were immunoprecipitated using GFP-Trap (Chromotek) and washed 3 times with 50mM ammonium bicarbonate after the precipitation to remove detergent ...
-
bioRxiv - Systems Biology 2023Quote: ... The proteins were immunoprecipitated using GFP-Trap (Chromotek) according to manufacturer’s protocol (with slight modifications) ...
-
bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates were cleared by centrifugation (20,000x g, 20 min) and incubated with GFP-Trap beads (ChromoTek) for 3 hr at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...
-
bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
-
bioRxiv - Developmental Biology 2019Quote: ... Tagged proteins were immunoprecipitated with Myc-Trap_MA beads (ChromoTek) or anti-FLAG M2 Affinity gel (Sigma ...
-
bioRxiv - Biophysics 2020Quote: ... they were incubated with unlabelled (GFP-binding protein, Chromotek) and Abberior 635P conjugated Nanobody (GFP-booster ...
-
bioRxiv - Cell Biology 2021Quote: ... protein lysates were added to RFP-Trap beads (Chromotek) and incubated for one hour at 4°C on a rotating shaker ...
-
Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rat anti-red fluorescent protein (RFP) monoclonal (5F8; ChromoTek GmbH ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were detected using anti-GFP antibody (3H9, Chromotek) or anti-alpha tubulin (T5168 ...
-
bioRxiv - Plant Biology 2023Quote: ... the purified recombinant mCherry or Tip6ΔSP-mCherry proteins were separately mixed with purified His-ZmTPL2N-His proteins and incubated with pre-washed magnetic mCherry-Trap beads (Chromotek, Cat. No. rtmak) in wash buffer (0.2 M NaH2PO4/0.2 M Na2HPO4 ...
-
bioRxiv - Plant Biology 2024Quote: ... Coimmunoprecipitation of GFP- or RFP-tagged proteins from total leaf protein extract by incubation with GFP- or RFP-Trap magnetic agarose beads (ChromoTek, Planegg-Martinsried Germany) was performed as previously reported (Azevedo et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP-tagged proteins were affinity purified by GFP-trap (ChromoTek) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... The protein complex pulled down by GFP-Trap beads (Chromotek) was washed three times with RIPA buffer and eluted in 30 μl of 1x Laemmli sample buffers by heating at 75°C for 10 min.
-
bioRxiv - Cell Biology 2022Quote: ... GFP-GABARAP proteins were immunoprecipitated using GFP-TRAP beads (ChromoTek), incubating for 2hrs at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
-
bioRxiv - Microbiology 2023Quote: GFP-tagged proteins were immunoprecipitated using GFP-TRAP beads (Chromotek) using TNTE buffer (20mM Tris-HCL pH 7.4 ...
-
bioRxiv - Cell Biology 2023Quote: ... or the 32F6 IgG2c monoclonal antibody against mNeonGreen protein (Chromotek). After several 5 min washes in PBS ...
-
bioRxiv - Genetics 2023Quote: Protein complex was purified using GFP-Trap agarose beads (Chromotek) and separated by western blot ...
-
bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
-
bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by GFP-Trap Agarose (Chromotek, gta).
-
bioRxiv - Plant Biology 2024Quote: ... then proteins were immunoprecipitated by RFP-Trap Agarose (Chromotek, rta). pFRETgc 35S::EGFP + 35S::BEN1-mCherry and pFRETgc 35S: ...
-
bioRxiv - Plant Biology 2024Quote: ... GFP-tagged proteins were detected by α-GFP antibodies (ChromoTek).
-
bioRxiv - Genomics 2019Quote: ... Cell lysates were cleared by centrifugation at 5,000 g for 5 min before incubation with 50 µl pre-washed GFP-TRAP MA beads (Chromotek) for 1 h at 4 degrees Centigrade ...
-
bioRxiv - Biochemistry 2021Quote: ... the lysates were centrifuged at 16,000 g for 60 min and the supernatant bound to 25 μl of GFP-trap magnetic beads (Chromotek) for 1 h with end-over-end rotation at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... The extracts were cleared by centrifugation at 8000 g for 10 min and supernatants were incubated with GFP-Trap_A matrix (ChromoTek) or anti-HA magnetic beads (Thermo Scientific ...