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Citations for ChromoTek :
1 - 50 of 59 citations for Legionella pneumophila antigen native extract since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were incubated with GFP-Trap (ChromoTek) beads for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Twenty milligram protein extracts were taken as input and the rest of the extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... extracts were incubated with GFP-Trap beads (Chromotek, Germany) for one hour ...
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bioRxiv - Cell Biology 2019Quote: ... A total of 4 mgs of protein extract per sample was then incubated with GFP-Traps beads (ChromoTek; 3 μl/mg of extract) for 3 hours at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were immunoprecipitated with GFP-Trap beads (Chromotek, Germany), and analyzed by western blot with anti-GFP (Abiocode ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were processed for co-immunoprecipitation using GFPTrap beads (Chromotek) (20µl GFPTrap beads per condition ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were then processed for coimmunoprecipitation using GFP-trap (Chromotek) following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... protein extracts were incubated with GFP-Trap Agarose (Chromotek, Germany) at 4°C for 1 h with gentle rotation ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were incubated with anti-GFP agarose beads (GFP-trap, Chromotek) for 2h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: Extracts containing GFP-fusion proteins were incubated on GFP-Trap agarose beads (Chromotek) for 20 minutes at 4°C on a rotating wheel in presence of Ethidium Bromide ...
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bioRxiv - Plant Biology 2021Quote: ... The nuclear extracts were incubated with 25 µL of GFP Magnetic Trap beads (Chromotek) according to manufactures instructions ...
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bioRxiv - Genetics 2022Quote: ... 1 mg of protein extracts was incubated with agarose GFP traps (Chromotek, #GTA-20) pre-equilibrated with Buffer D (20mM HEPES pH 7.9 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extract was incubated with 40 uL of GFP-trap MA bead slurry (ChromoTek), shaking for 2 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of extract protein were incubated with GFP-binding protein-conjugated agarose (Chromotek) or anti-RFP antibody (Anti-RFP RTU - Rockland Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... Extracts were then treated with The DYDDDDK Fab-Trap™ agarose beads (ChromoTek, Proteintech) and the retained Flag-tagged FlaA/B was eluted from the agarose beads using 3xFLAG®-peptide (Chromotek ...
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bioRxiv - Cancer Biology 2023Quote: ... Total protein extracts were incubated with goat anti-GFP Trap agarose (gta-20, Chromotek) or anti-c-Myc Agarose (20168 ...
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bioRxiv - Plant Biology 2024Quote: ... The filtered extracts were incubated with 15 µL GFP-trap agarose beads (ChromoTek, Germany) at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... 100 μg SIRK1-GFP protein extract was prebound to GFP-Traps®-MA beads (Chromotek) for two hours under rotation at 4 °C ...
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bioRxiv - Plant Biology 2020Quote: ... and then the rest extracts were incubated with the GFP-Trap beads (ChromoTek, gta-20) for 1.5 h at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... precleared cell extracts (10,000, 5 min) and incubated the supernatants with Myc-trap resin (Chromotek) for 3 hours at 4 °C.
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bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
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bioRxiv - Plant Biology 2022Quote: ... clarified extracts were incubated with GFP-TRAP Magnetic Agarose affinity beads (ChromoTek GmbH, Planegg-Martinsried, Germany) for one hour ...
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bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... with equal amounts of total protein extract for each condition incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Plant Biology 2022Quote: ... leaf material was harvested and protein extracts were subjected to immunoprecipitation using GFP-Trap beads (ChromoTek). 200 mg ground leaf material was subjected to protein extraction and immunoprecipitation using GFP-Trap beads (ChromoTek)19 ...
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bioRxiv - Molecular Biology 2023Quote: ... MNase-digested nuclear extracts were incubated overnight with GFP-Trap® Magnetic Particles M-270 (Chromotek) rotating at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Plant Biology 2021Quote: ... The protein extracts were incubated with 20 μL of GFP-Trap magnetic agarose beads (GFP-TrapMA, Chromotek) for 2 h at 4 °C by vertical rotation (10 rpm) ...
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bioRxiv - Plant Biology 2021Quote: ... To each 15 ml of protein extract 40 μl of GFP-TRAP AGAROSE BEADS (50 % slurry, ChromoTek) washed in extraction buffer were added and incubated with gentle agitation for 4 h at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... were co-precipitated from cells extracts by pulling-down the GFP moiety with GFP-Trap® (Chromotek), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Twenty μl of anti-GFP beads was mixed with 1.4 ml of filtered extracts (Chromotek, Munich, Germany) and incubated at 4□ for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... an equal amount of total protein extract for each condition was incubated with GFP-Trap_A beads (Chromotek) at 4°C for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... the GFP-fused substrate proteins were immunoprecipitated from the cell extract using GFP-Trap A beads (Chromotek) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The extract was pre-cleared with Sepharose beads and subjected to immunoprecipitation with GFP-Trap beads (Chromotek). After extensive washing with RIP washing buffer ...
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bioRxiv - Genetics 2020Quote: ... Clarified extract was incubated with 15 μl GFP-TRAP-A beads or blocked agarose beads (bab-20) (Chromotek) equilibrated in C Buffer for 2 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... For IP 2 mg of cytoplasmic and nuclear extracts were incubated with either 15 µl GFP Trap beads (Chromotek) for 1 h at 4°C or 1-1.5 μg IgG overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... GFP fusion proteins were purified as follows: 200mg of protein extract was incubated with GFP-Trap Magnetic Agarose (ChromoTek) at 4°C for 20 minutes with gentle agitation ...
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bioRxiv - Cell Biology 2019Quote: Soluble fractions of cell extracts were immunoprecipitated with an anti-GFP antibody coupled to magnetic beads (GFP-Trap_MA, ChromoTek) or an anti-HA antibody coupled to agarose beads (EZview Red Anti-HA Affinity Gel ...
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bioRxiv - Molecular Biology 2023Quote: Preparation of DNase-digested cell extracts and immunoprecipitation was done using GFP-Trap® Magnetic Particles M-270 (Chromotek) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 100 µg for the input were saved and then cell extracts were immunoprecipitated by a GFP-Trap (Chromotek, Germany). As a negative control for pull-downs assays the eGFP plasmid (Clontech Laboratories ...
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bioRxiv - Biochemistry 2021Quote: Protein extracts containing GFP-tagged EFR or site-directed mutants were incubated with 20 μL of GFP-Trap beads (Chromotek) or GFP-clamp beads (Hansen et al. ...
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bioRxiv - Cell Biology 2021Quote: ... or chd-1(bs125) (CHD-1::sfGFP) whole worm extracts and 80 microliters of GFP-Trap Magnetic Agarose beads (Chromotek) were used for each IP ...
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bioRxiv - Molecular Biology 2022Quote: The rest of the extract was then incubated for 90 minutes with 10 μl slurry of GFP-Trap Agarose beads (gta-100, Chromotek). The beads were washed four times with 1 ml of Wash buffer and bound proteins were eluted at 95 °C for 3 minutes in 30 μl of 1X LDS sample loading buffer.
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bioRxiv - Molecular Biology 2020Quote: ... 450 μl of nuclear extract was used for each GFP pull down using 15 μl slurry of GFP-Trap agarose beads (Chromotek), performed in triplicate ...
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bioRxiv - Molecular Biology 2021Quote: ... the extract prepared from OSC lines expressing endogenously tagged proteins of interest was incubated with magnetic GFP-Trap agarose (Chromotek) for 3h at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... The immunoprecipitation was performed using 3 mL of plant extract mixed with 25 µL of GFP-trap magnetic beads (Chromotek) at 4 °C overnight ...
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bioRxiv - Plant Biology 2023Quote: GFP-tagged or RFP-tagged proteins were immunoprecipitated from total leaf protein extract by incubation with GFP-Trap or RFP-Trap magnetic agarose beads (ChromoTek). The Co-immunoprecipitation assay was carried out according to ChromoTek’s instructions whereby 300 μl of protein extract was diluted with 500 μl of ice-cold dilution buffer [10 mM Tris-HCl (pH 7.5),150 mM NaCl ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mg of nuclear extract (nuclear-soluble and chromatin-bound fractions were pooled together) was incubated with Agarose GFP traps (Chromotek) or anti-HA Affinity Matrix (Roche ...
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bioRxiv - Biochemistry 2024Quote: Protein extracts containing GFP-tagged MIK2 or site-directed mutants were incubated with 20 µL of GFP-Trap beads (Chromotek) for 2 h with gentle mixing at 4 °C to immuno-precipitate receptor complexes ...