Labshake search
Citations for ChromoTek :
1 - 50 of 507 citations for Cow G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2019Quote: ... GFP tagged proteins was detected with GFP-booster coupled with Atto488 (1:500; Chromotek). DNA was stained by DAPI (0.2 µg/ml ...
-
bioRxiv - Plant Biology 2024Quote: Immunoprecipitation was performed by adding 1 g of homogenized leaf material 50 µL of GFP-Trap® coupled to magnetic beads (ChromoTek). Samples were processed as described (Raffeiner et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Protein extracts were then subjected to immunoprecipitation using agarose beads coupled with anti-RFP nanobodies (Chromotek). Eluates were collected and analysed by western blot ...
-
bioRxiv - Neuroscience 2020Quote: ... approximately 500 µg of cellular protein was incubated with 1 ug of flag antibody or 20 µL of RFP-Trap®_MA (Chromotek). The magnetic beads were collected using a magnet and washed three times in NP-40 lysis buffer ...
-
bioRxiv - Systems Biology 2022Quote: ... and 1:500 anti-rabbit nanobody coupled to Alexa Fluor 647 (Chromotek srbAF647-1-100). Slides were washed three times for two minutes in PBS in the dark ...
-
bioRxiv - Molecular Biology 2020Quote: ... The remaining supernatants were used for immunoprecipitation of GFP-fused proteins agarose using GFP-Trap coupled to agarose beads (Chromotek) and following the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: The one-step immunoprecipitation of GFP-tagged proteins is performed by anti-GFP VHH coupled agarose beads (90 μm) (Chromotek) with a binding capacity of 3-4 μg/10 μl GFP-Trap® slurry ...
-
bioRxiv - Neuroscience 2023Quote: ... Anti-GFP VHH coupled to agarose beads for immunoprecipitation of GFP-fusion proteins (GFP-Trap; ChromoTek GmbH, Germany; gta-20) was used for immunoprecipitation of mCerN1 ...
-
bioRxiv - Neuroscience 2020Quote: ... GFP fusion proteins in fixed cells were labelled with Alexa Fluor 647-coupled GFP nanobodies (GFP-booster gb2AF647-50, Chromotek, Germany) at 1:100 dilution ...
-
bioRxiv - Plant Biology 2021Quote: One milligram of total protein extract was mixed with 25 μl of GFP Nanobody/VHH coupled to agarose beads (GFP-Trap® Agarose, Chromotek) and incubated for 3 hours and 30 minutes at 4°C with end to end rocking ...
-
bioRxiv - Developmental Biology 2021Quote: ... The supernatant was subsequently used for the Co-IP using a GFP-binding protein coupled to magnetic beads (GFP-Trap_M; ChromoTek, Planegg-Martinsried) following manufacturers instructions ...
-
bioRxiv - Microbiology 2020Quote: ... antibody and protein G beads and GFP-ARTD12 with 5 µl of GFP-Trap magnetic agarose beads (Chromotek) at 4 °C for 1 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... α-GFP antibody-coupled magnetic beads (Chromotek) were equilibrated with IP buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... The lysates were cleared by centrifugation (13000 g, 30 min) and the proteins were captured with GFP-trap (Chromotek), Myc-trap (Chromotek ...
-
bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... To enhance signal from fluorescent reporters we used GFP-booster coupled to Atto488 (gba488) and RFP-booster coupled to Atto594 (rba594) from Chromotek. Secondary antibodies used were from Thermo Scientific ...
-
bioRxiv - Plant Biology 2023Quote: ... or Myc-antibody coupled beads (Myc-trap, ChromoTek) were used ...
-
bioRxiv - Neuroscience 2020Quote: ... with rotation with anti-GFP-coupled magnetic beads (Chromotek). The beads were washed with lysis buffer and 1 M urea before Western blot analysis ...
-
bioRxiv - Cell Biology 2023Quote: ... One third of the whole cell lysate was pre-cleared with a 50:50 mixture of protein A/G magnetic beads and GFP-Trap (Chromotek, gtd-20) was pre-blocked with 1mg/mL ultra-pure BSA (AM2616 ...
-
bioRxiv - Neuroscience 2022Quote: ... rat monoclonal anti-red fluorescent protein (RFP) (Chromotek, 1:1000) and goat anti-mCherry (1:1000) ...
-
bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitation was performed using the protein lysates and a GFP-Trap_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed using the protein lysates and a GFP-Trap_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... red fluorescent protein (RFP; rat, Chromotek: 5F8, RRID: AB_2336064; 1:2000) FoxP2 (mouse ...
-
bioRxiv - Cell Biology 2021Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Immunoprecipitation was performed using crosslinked protein and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... and a red fluorescent protein tag (tdTomato; ChromoTek, 5f8-100, 1:500) expressed by the rabies virus ...
-
bioRxiv - Cell Biology 2019Quote: ... nanobody boosters towards GFP (anti-GFP, Atto-488 coupled, Chromotek; gba488-100) were used to enhance the signal ...
-
bioRxiv - Cell Biology 2021Quote: ... cells were incubated with RFP-Booster nanobody coupled to Atto 594 (Chromotek) at a dilution of 1:200.
-
bioRxiv - Cell Biology 2020Quote: ... Immunoprecipitation was performed using crosslinked protein lysate and a GFP-Trap®_A Kit (Chromotek) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... mCherry-fusion proteins were isolated by immunoprecipitation using the RFP-Trap®_MA kit (Chromotek). Lysates from transfected cells were prepared as above and incubated with RFP-trap magnetic agarose beads for 30 min to capture mCherry or mCherry-Lpg2411 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Lysates from cells expressing GFP tagged proteins were immunoprecipitated using a GFP-Trap Kit (Chromotek) as per manufacturer’s instructions and immunoblotting performed as above ...
-
bioRxiv - Plant Biology 2019Quote: ... After centrifugation the cleared extracts (15 µg of proteins) were fractionated in a 10% polyacrylamide gel and protein immunodetection was realized with an antibody anti GFP (PABG1, Chromotek, 1/3000).
-
bioRxiv - Molecular Biology 2022Quote: ... Supernatants were incubated 1h with GFP-Trap coupled to magnetic agarose beads (Chromotek). Then ...
-
bioRxiv - Genetics 2022Quote: ... 1 mg of protein extracts was incubated with agarose GFP traps (Chromotek, #GTA-20) pre-equilibrated with Buffer D (20mM HEPES pH 7.9 ...
-
bioRxiv - Plant Biology 2019Quote: ... RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000; ChromoTek). Light harvesting complex protein (LhcB1 ...
-
bioRxiv - Plant Biology 2019Quote: ... or an anti-Myc-tag Nanobody coupled to agarose (Myc-Trap, Chromotek, Hauppauge, NY) for 1 hour at 4 °C ...
-
bioRxiv - Plant Biology 2019Quote: ... mCherry proteins were detected by a rat anti-RFP antibody (5F8-100, 1:2,000, Chromotek), and then with a goat anti-rat IgG HRP-conjugated secondary antibody (AP202P ...
-
bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was then incubated with anti-GFP coupled magnetic microparticles (GFP-Trap, ChromoTek) for 2 h followed by four washing steps ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation was performed by adding 30 mL of GFP-Trap coupled to agarose beads (ChromoTek) and samples were incubated for 2 h at 4°C with continual rotation ...
-
bioRxiv - Cell Biology 2023Quote: Affinity purification was performed using anti-GFP nanobody coupled magnetic beads (∼ 30µl slurry) (Chromotek AB_2631358). Briefly ...
-
bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation was performed by adding 30 μl of GFP-Trap coupled to agarose beads (ChromoTek) and samples were continuously agitated for 2 hours at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Supernatant was applied onto GFP-nanobodies coupled to magnetic agarose beads (GFP-trap, ChromoTek, Germany) equilibrated in lysis buffer ...
-
bioRxiv - Biochemistry 2019Quote: ... Protein (1 mg/mL) was incubated with 25 μL of GFP-Trap magnetic micro beads (ChromoTek), or anti-FLAG M2 magnetic beads (Sigma) ...
-
bioRxiv - Plant Biology 2020Quote: ... Protein extracts were incubated for 1 h with 25 μl GFP trap (ChromoTek; Planegg-Martinsried, Germany) on a rotating wheel at 4°C ...
-
bioRxiv - Plant Biology 2023Quote: ... GFP tagged proteins were detected using an anti-GFP antibody (1:4000; mAb rat 3H9; Chromotek) and a secondary anti-rat antibody (1:15000 ...
-
bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 mg total proteins were incubated with 15 µl pre-equilibrated magnetic GFP-TRAP beads (Chromotek) for 2 h on a rotator at 4 °C ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by incubation of the cleared supernatant with 10 µL prewashed GFP-coupled magnetic agarose (Chromotek) per 10 cm dish ...
-
bioRxiv - Plant Biology 2021Quote: ... Immunoprecipitation was performed by adding 30 µL of GFP-Trap® coupled to magnetic beads (ChromoTek) and samples were incubated for 2 h at 4°C with continual rotation ...