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Citations for ChromoTek :
1 - 50 of 343 citations for 1 2 2 morpholin 4 ium 4 ylethoxy phenyl butan 1 one chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... protein extracts were incubated for 1-2 h at 4°C with GFP-Trap_MA (magnetic beads, ChromoTek, handling according to manufacturer’s manual) ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated 1 h at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated overnight or 2 nights at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Plant Biology 2024Quote: ... 1 mL of supernatant was incubated at 4°C for 2 h with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany). The beads were collected by centrifugation at 800 × g and washed four times in 1 mL washing buffer (25 mM Tris pH=7.5 ...
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bioRxiv - Developmental Biology 2021Quote: ... two different protocols were used: 1) one step of O/N incubation at 4°C with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Plant Biology 2021Quote: ... and serine 2 (Chromotek, diluted 1:200). After washing with PBS ...
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bioRxiv - Plant Biology 2019Quote: ... Supernatants were incubated (2 h, at 4°C) with 20 µl GFP-Trap®_MA (ChromoTek). Beads were then washed 3 times for 10 min with 1 ml of IP buffer and eluted with Laemmli sample buffer ...
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bioRxiv - Pathology 2021Quote: ... 1-4 mL of supernatant was incubated at 4°C with GFP-Trap_A beads (Chromotek, Planegg-Martinsried, Germany) overnight ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was then transferred in 900 μl aliquots to fresh Eppendorf tubes and incubated with gentle head-over-tail agitation for 2 h at 4°C with 30 μl of anti-RFP or control beads (Chromotek rtma-2 and bmab-20, respectively) that had been washed in 1x lysis buffer.
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bioRxiv - Cell Biology 2019Quote: ... lysates were incubated for 2 hours at 4°C with 25 μL GFP-Trap coupled agarose beads (Chromotek). After incubation ...
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bioRxiv - Genomics 2020Quote: ... at 4°C or for 2 hours with 20 uL of GFP-Trap magnetic beads (Chromotek, gtma-20) at 4°C ...
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bioRxiv - Systems Biology 2021Quote: ... Protein concentration was evaluated with a BCA dosage and 1 mg of total protein was incubated at 4 °C for 4 hours with agarose GFP beads (ChromoTek, Germany) for PHB-GFP or with magnetic FLAG beads (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 15 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were then incubated for 2 hours at 4°C with 150 μL GFP-Trap coupled to agarose beads (Chromotek) in an end-over-end rocker ...
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bioRxiv - Cell Biology 2024Quote: ... 0.6 mL of the resulting supernatant (containing 2 – 4 mg of proteins) were supplemented with 15 μL of RFP-Trap magnetic particles (ChromoTek), and incubated for 2.5 h at 4°C on a rotating wheel ...
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bioRxiv - Plant Biology 2024Quote: ... Protein extracts were there incubated at 4 C for 1 h with GFP-Trap beads (Chromotek) and unspecific binding was minimized by several washes with wash buffer (the same as extraction buffer but with only 0.2 % NP-40) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was incubated at 4°C for 1 h with GFP-Trap Agarose beads (Chromotek) and washed 4 times ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were left on ice for 30 minutes and centrifuged at 16,000 g for one hour at 4 °C and the protein was captured with 25 μL Myc-trap (Chromotek) beads by tumbling overnight ...
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bioRxiv - Plant Biology 2021Quote: ... The filtered supernatant was then incubated for one hour at 4°C with 50 μL of anti-GFP agarose beads (Chromotek) to pull down GRP7-GFP and with 50 μL of anti-HA agarose beads (ThermoFisher ...
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bioRxiv - Developmental Biology 2021Quote: ... and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6], Chromotek, 1:250) diluted in PBT + 1x WBR ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then probed overnight at 4°C with TBST buffer containing 1% non-fat dry milk and primary antibodies: mouse monoclonal anti-RFP (Chromotek, clone 6G6, 1:3,000), rabbit polyclonal anti-GFP (SYSY ...
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bioRxiv - Plant Biology 2020Quote: ... Tagged proteins were immunoprecipitated for 1 h at 4°C using GFP-Trap beads (ChromoTek, Planegg, Bavaria, Germany) following the method of Chinchilla et al5 or for Fig ...
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bioRxiv - Microbiology 2019Quote: ... and then diluted 1/4 in dilution buffer with 0.025% Tween-20 and added to washed GFP-Trap_MA beads (Chromotek). For the negative control ...
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bioRxiv - Molecular Biology 2022Quote: ... rat antibodies for the detection of phosphorylated RNAPII (serine 5 and serine 2) (Chromotek, 1: 100) and secondary goat anti-rat Alexa Fluor 488 antibodies (A-11006 ...
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bioRxiv - Cell Biology 2022Quote: ... EGFP-INPP5E or its mutants were then immunoprecipitated from 1 mg of cell lysate by overnight rotation at 4°C with GFP-Trap_MA beads (Chromotek). Beads were then washed thrice in buffer containing 50mM Tris-HCl pH=7.5 and 150mM NaCl buffer (no protease inhibitors or detergent) ...
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bioRxiv - Biophysics 2023Quote: ... 0.25 mM PMSF) and eluted with 4 × 1 mL 0.15 mg/mL (50 µM) 3 x FLAG peptide (Chromotek). The elutant was concentrated with Amico-4 (50k MWCO ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at +4°C with the primary antibody (rabbit anti-GFP, 1:600, Chromotek) diluted in the blocking solution ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 4 hours at 4°C with agarose-conjugated GFP-Trap™ beads (Chromotek). Beads were washed once with lysis buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... on a rotating wheel at 4° C for 1 h in triplicates with GFP-Trap agarose beads (#gta-200, Chromotek) or control agarose beads (Chromotek) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated 1-2 hrs at room temperature in a blocking solution containing 3% Normal Donkey serum and 0.3% Triton X-100 in PBS and incubated 48 hrs at 4°C with primary antibodies: rat anti-RFP (1:1,000; Chromotek #5f8), chicken anti-GFP (1:1,000 ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... incubated with a 1:1,000 dilution of mouse anti-GFP [B-2] (SantaCruz Biotechnology) or a rat anti-RFP [5F8] (Chromotek) followed by a 1:5,000 dilution of the horseradish peroxidase-conjugated secondary antibody against mouse IgG (7076 ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were precleared with 50 µL of sepharose beads for 1 h at 4°C with constant rotation and subjected to immunoprecipitation with 25 µL GFP Trap agarose beads (Chromotek, gta-20) during 1 h at 4°C with constant rotation ...
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bioRxiv - Plant Biology 2020Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Plant Biology 2022Quote: ... sample supernatants were adjusted to 2mg/ml protein and incubated 4 hours at 4°C with GFPTrap-A beads (Chromotek). Beads were washed 4 times with wash buffer (20 mM Tris pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... transduced cells were incubated with 100 μL Hybridization Buffer containing 2 μL 12.5 μM CF568-labled gRNA FISH probes and 1:2000 AF647-labeled anti-GFP nanobody (Chromotek, gb2AF647-50) for 4 hours in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... one step of O/N incubation at 4ºC with GFP booster (1:250, Alexa Fluor ® 488 Chromotek #gb2AF488), Phalloidin-647 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight at 4°C in primary antibodies (RFP from Chromotek 5f8-100 at 1:200 ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated at 4°C overnight with GFP-Trap agarose (Chromotek) or Dynabeads (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) beads as described by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Complexes were immunoprecipitated at 4°C in lysis buffer with GFP-Trap (ChromoTek) and washed in lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Myc-Trap agarose beads A (Chromotek, yta-2 Myc Trap_A) were prepared by 3 rounds of washing in extraction buffer and centrifugation at 1000 x g for 5min at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 4 mg lysate was incubated with 10 μl RFP-Trap magnetic agarose beads (Chromotek) for 3 h at 4 °C ...
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bioRxiv - Cell Biology 2019Quote: ... Complexes were immunoprecipitated for 90 minutes at 4°C with GFP-Trap beads (Chromotek). After five washes in lysis buffer ...
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bioRxiv - Microbiology 2020Quote: ... 4°C) and the supernatant incubated with 25 μl of equilibrated GFP-Trap resin (ChromoTek) for 2 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed before incubated with GFP-Trap Agarose beads overnight at 4 °C (Chromotek) and washed as manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... at 4°C) and the supernatant incubated with anti-GFP TRAP magnetic agarose beads (ChromoTek) for 1 hr at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... were immunoprecipitated from 2 mg of lysates using GFP-Trap beads (Chromotek) at 4°C for 4h under rotation ...
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bioRxiv - Genetics 2019Quote: ... Immunoprecipitations were performed on a rotating wheel at 4°C overnight with GFP-Trap beads (Chromotek). The beads were then washed 4 times with lysis buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Lysates were cleared by centrifugation (6,000 g, 30 min, 4°C) and incubated with GFP-Trap (Chromotek) for 2h ...