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Citations for Biovision :
1 - 50 of 183 citations for Recombinant Mouse Programmed Cell Death 1 Ligand 2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: ... his-tagged extracellular domain of the growth hormone receptor (GHR, BioVision 7477-10) was immobilized on the Ni-NTA sensor tip by dipping the tips in a 2.5 μg/ml solution of GHR for 400 s ...
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bioRxiv - Cancer Biology 2023Quote: ... The HIS-tagged protein was purified on Ni-NTA (cat. no. 6562; BioVision; abcam, Inc.) column ...
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bioRxiv - Cancer Biology 2023Quote: ... and Flt-3 ligand (Biovision). These cultures were maintained by changing media every day ...
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bioRxiv - Cell Biology 2019Quote: ... and 10ng/ml IGF-1 (recombinant human, BioVision, San Francisco, CA). Media was changed on alternate days over a 10-day period.
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bioRxiv - Cell Biology 2022Quote: ... were assessed by terminal deoxynucleotidyltransferase-mediated dUTP nick end labeling (TUNEL) assay with an in situ Cell Death Detection Kit (Biovision Inc., CA, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... 1% PS and 5ng/ml human recombinant TGF-β1 (Biovision, 4342-5). This medium was changed every 4 days and after 14 days ...
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bioRxiv - Immunology 2021Quote: ... 100 µl of rabbit anti-His-tag antibody (1:1000, BioVision, USA, cat#3998) was incubated at 37℃ for 2 h and washed as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant Human Wnt 3a (BioVision) was administered at 150 ng/ml for 24 hours ...
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bioRxiv - Genetics 2023Quote: ... 1% P/S and 5ng/ml active human recombinant TGF-β1 (Biovision, 4342-5). This medium was replaced every 4 days ...
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bioRxiv - Molecular Biology 2019Quote: ... and recombinant active caspase 3 (0.5 μg; Chemicon) or recombinant active caspase 7 (0.5 μg; Biovision) were incubated for 3 h in cleavage assay buffer (50 mM Hepes ...
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bioRxiv - Immunology 2019Quote: ... cultures were supplemented with recombinant SCF (50ng/ml; BioVision), G-CSF (20ng/ml ...
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bioRxiv - Immunology 2021Quote: ... 10 μM PS341 and 10 μM PR619 for 15 minutes on ice followed by addition of 5 Units of active recombinant caspase-1 (Biovision). This lysate was incubated at 37 °C for 80 minutes and samples subsequently diluted in ice-cold DISC buffer (containing 2 mM NEM and Roche complete protease-inhibitor cocktail) ...
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bioRxiv - Microbiology 2020Quote: ... cells growing on coverslips transfected with plasmids or infected with EBOV were incubated in serum-free-(SF)-DMEM for 1 h and treated without or with 10 ng/mL recombinant human OSM (BioVision) for 15 min (analysis of fixed/immunostained cells ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human IFN-α2a was purchased from BioVision (Milpitas, CA). In the present experiments Huh7 cultures were exposed for two days to IFN-α at a concentration of 3,000 IU/ml prior in DMEM supplemented with 2% FBS prior to prior to fixation and immunofluorescence analyses (22).
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human IFN-α2 was purchased from BioVision (Milpitas, CA) and typically used at 10-20 ng/ml for 2 days ...
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bioRxiv - Biophysics 2023Quote: ... or Halo-TM-ABD* and labelled with Halo ligand were imaged at 37°C using an S-TIRF system (Spectral Applied Research, via BioVision Technologies, Exton, PA) mounted on a Nikon Ti-E inverted microscope with Perfect Focus and a 60x/1.49 NA oil TIRF (total internal reflection fluorescence ...
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bioRxiv - Biophysics 2021Quote: The PSF was characterised using purified recombinant EGFP (Biovision, Milpitas, California) diluted to a final concentration of 20 mM in PBS (Gibco ...
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bioRxiv - Immunology 2020Quote: Infections were performed as described above except KSHV.219 virus was pre-incubated for 30 minutes on ice with serum free RPMI only or serum free RPMI containing recombinant human syndecan-1 protein (rhCD138, BioVision, 7879-10) prior to being added to Naïve B lymphocytes ...
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bioRxiv - Synthetic Biology 2022Quote: ... the 10 % PVA inner phase was supplemented with 32.7 kDa recombinant EGFP (Biovision) mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were differentiated into mature macrophages with human recombinant macrophage colony stimulating factor (BioVision) in RPMI 1640 media supplemented with 20% human AB serum (Corning ...
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bioRxiv - Cell Biology 2020Quote: 2×105 cells were subjected to the Caspase-3/CPP32 Fluorometric Assay Kit (BioVision) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were then loaded with Fura-2-AM (5 μM, 37 °C, Biovision, #125280) for 45min and washed with Standard Extracellular Solution (145 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... four-micron sections of formalin-fixed paraffin-embedded tissue were mounted on positively charged Superfrost® Plus slides and subjected to IHC using a SARS-CoV-2-specific anti-nucleocapsid mouse monoclonal antibody (clone 6F10, BioVision, Inc., Milpitas, CA, USA) as previously described (Carossino et al. ...
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bioRxiv - Plant Biology 2020Quote: ... LED light of 600 μmol m−2 s−1 (pE-100wht, BioVision Technologies, Exton, PA, USA) was used for actinic light ...
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bioRxiv - Neuroscience 2020Quote: ... Thiazovivin (2 μM, BioVision) was supplemented into the seeding media ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were stained with 10 µL of WST-1 reagent (Biovision) at 37°C for 30 minutes in the dark and analyzed at 450 nm with 96-well plate reader (Allsheng ...
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bioRxiv - Neuroscience 2020Quote: ... and incubation with the primary antibody (polyclonal rabbit anti-dsRed antibody, 632496, Clontech, dilution 1:500; monoclonal mouse anti-dsRed antibody, 3994-100, BioVision, dilution 1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were assayed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) tetrazolium reduction assay (BioVision, Milpitas, CA) according to manufacturer instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and 2× Reaction Buffer (BioVision) in a black microplate and incubated at 37℃ for 2 h ...
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bioRxiv - Bioengineering 2022Quote: Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
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bioRxiv - Cancer Biology 2020Quote: ... mouse p53 (BioVision, Cat # 3036-100), p21 (Abeam ...
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bioRxiv - Microbiology 2023Quote: ... Mouse anti-GRA5 antibody (BioVision, #A1299) (1:500 ...
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bioRxiv - Bioengineering 2022Quote: Naïve and provisional mouse tendons were decellularized using an adapted detergent-free decellularization method29,30 for skeletal muscle31 based on Latrunculin A (Biovision, #2182-1) and alternating hypertonic-hypotonic solutions ...
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bioRxiv - Cancer Biology 2020Quote: ... we suspended 2 × 106 cancer cells cultured in 2 mL of D0 medium with or without the SYK inhibitor R406 (10 µM; BioVision, 9682); the PKC inhibitor (30 µM ...
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bioRxiv - Bioengineering 2022Quote: Drugs were added to organoids concurrently with IL-1 β on Days 2 and 4 upon media replacement: Tocilizumab (Cat: A1447-200, Lot: 6G02A14470, Biovision), Human IL-1RA (Cat:4263-100 ...
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bioRxiv - Neuroscience 2022Quote: Mouse plasma samples were diluted 1:100 in the provided dilution buffer and measured using the ALP ELISA kit (Biovision #E4572-100) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2024Quote: ... 400mM 2-Deoxy-D-Glucose (BioVision, #B1048), 400mM 3-Bromopyruvic acid (BioVision ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IL-1β antibody (5129-100, BioVision); anti-mouse Caspase-1 p20 (AG-20B-0042-C100 ...
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bioRxiv - Microbiology 2021Quote: ... or anti-SARS-CoV-2 Nucleoprotein (N; BioVision), and rabbit anti-DDX42 antibody (HPA023571 ...
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bioRxiv - Developmental Biology 2020Quote: ... and 2-inhibitor cocktail (3 mM CHIR (BioVision) and 1 mM PD0325901 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were counterstained with Hematoxylin (BioVision, K580-24-2) for 1 min ...
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bioRxiv - Developmental Biology 2022Quote: ... RANKL and insulin were measured by mouse Osteocalcin ELISA Kit (BioVision), OPG ELISA Kit (Boster Biological Technology) ...
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bioRxiv - Cell Biology 2022Quote: ... and Necrostatin-1 (Nec-1, BioVision). For inhibition experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... BV2 cells were incubated in 1 µM non-fluorescent Aβ for two hours then an EZCell™ Protein Transport Inhibitor Cocktail (BioVision #K932-500) which disrupts the structure and function of the Golgi apparatus and allows cytokines to be retained intracellularly ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake analysis was performed with a 2-NBDG Glucose Uptake Assay Kit (BioVision) per the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... A8674, Sigma), 10mM glucose (A2494001, Thermo Scientific and 50mM 2-DG (B1048-100, BioVision) as indicated ...
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bioRxiv - Cancer Biology 2024Quote: Cell viability for TNBC cell lines following drug treatment was determined using colorimetric cell proliferation assays (MTS, BioVision Inc) per manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... Bioluminescence imaging was performed by injection of 200 μL (2 mg/mL) D-luciferin (BioVision) per mouse ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM β-mercaptoethanol and 0.003125% [w/v] NP-40 (#S226, Biovision Detergents Pak I) to set up cryo-EM grids ...
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bioRxiv - Physiology 2021Quote: ... rabbit anti-caspase-1(1:200; Biovision Research Products, CA). After washing ...