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Citations for Biovision :
1 - 50 of 67 citations for Recombinant Human GAB1 Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The HIS-tagged protein was purified on Ni-NTA (cat. no. 6562; BioVision; abcam, Inc.) column ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant Human Wnt 3a (BioVision) was administered at 150 ng/ml for 24 hours ...
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bioRxiv - Synthetic Biology 2022Quote: ... his-tagged extracellular domain of the growth hormone receptor (GHR, BioVision 7477-10) was immobilized on the Ni-NTA sensor tip by dipping the tips in a 2.5 μg/ml solution of GHR for 400 s ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human IFN-α2a was purchased from BioVision (Milpitas, CA). In the present experiments Huh7 cultures were exposed for two days to IFN-α at a concentration of 3,000 IU/ml prior in DMEM supplemented with 2% FBS prior to prior to fixation and immunofluorescence analyses (22).
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bioRxiv - Biochemistry 2022Quote: ... Recombinant human IFN-α2 was purchased from BioVision (Milpitas, CA) and typically used at 10-20 ng/ml for 2 days ...
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bioRxiv - Cell Biology 2019Quote: ... and 10ng/ml IGF-1 (recombinant human, BioVision, San Francisco, CA). Media was changed on alternate days over a 10-day period.
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bioRxiv - Immunology 2020Quote: Infections were performed as described above except KSHV.219 virus was pre-incubated for 30 minutes on ice with serum free RPMI only or serum free RPMI containing recombinant human syndecan-1 protein (rhCD138, BioVision, 7879-10) prior to being added to Naïve B lymphocytes ...
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bioRxiv - Genetics 2023Quote: ... 1% PS and 5ng/ml human recombinant TGF-β1 (Biovision, 4342-5). This medium was changed every 4 days and after 14 days ...
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bioRxiv - Microbiology 2021Quote: ... PBMCs were differentiated into mature macrophages with human recombinant macrophage colony stimulating factor (BioVision) in RPMI 1640 media supplemented with 20% human AB serum (Corning ...
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bioRxiv - Genetics 2023Quote: ... 1% P/S and 5ng/ml active human recombinant TGF-β1 (Biovision, 4342-5). This medium was replaced every 4 days ...
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bioRxiv - Microbiology 2020Quote: ... cells growing on coverslips transfected with plasmids or infected with EBOV were incubated in serum-free-(SF)-DMEM for 1 h and treated without or with 10 ng/mL recombinant human OSM (BioVision) for 15 min (analysis of fixed/immunostained cells ...
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bioRxiv - Molecular Biology 2019Quote: ... and recombinant active caspase 3 (0.5 μg; Chemicon) or recombinant active caspase 7 (0.5 μg; Biovision) were incubated for 3 h in cleavage assay buffer (50 mM Hepes ...
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bioRxiv - Immunology 2019Quote: ... cultures were supplemented with recombinant SCF (50ng/ml; BioVision), G-CSF (20ng/ml ...
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bioRxiv - Biophysics 2021Quote: The PSF was characterised using purified recombinant EGFP (Biovision, Milpitas, California) diluted to a final concentration of 20 mM in PBS (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 100 µl of rabbit anti-His-tag antibody (1:1000, BioVision, USA, cat#3998) was incubated at 37℃ for 2 h and washed as described above ...
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bioRxiv - Synthetic Biology 2022Quote: ... the 10 % PVA inner phase was supplemented with 32.7 kDa recombinant EGFP (Biovision) mixed to a final concentration of 2 µg/mL or 60 kDa FITC-labeled streptavidin (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... lyophilized human MSTN proform peptide (BioVision, 4623P-10) was reconstituted in ddH2O to a concentration 100 ng/μl ...
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bioRxiv - Bioengineering 2022Quote: Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
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bioRxiv - Bioengineering 2022Quote: ... Human IL-1RA (Cat:4263-100, Lot: 8L024263, Biovision), and Dexamethasone (Cat ...
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bioRxiv - Molecular Biology 2019Quote: ... we extracted CY and PM proteins using the PM Protein Extraction Kit (BioVision). The protein samples were treated for 30 min at 50°C in a sample-treating solution containing 2% SDS and 5% β-mercaptoethanol ...
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bioRxiv - Physiology 2023Quote: ... Soluble host protein was quantified in triplicate with a BCA Protein Assay Kit II (BioVision) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... 10 μM PS341 and 10 μM PR619 for 15 minutes on ice followed by addition of 5 Units of active recombinant caspase-1 (Biovision). This lysate was incubated at 37 °C for 80 minutes and samples subsequently diluted in ice-cold DISC buffer (containing 2 mM NEM and Roche complete protease-inhibitor cocktail) ...
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bioRxiv - Cell Biology 2022Quote: ... human chylomicrons (0.89 mg/ml, catalog no. 7285-1000, Biovision Incorporated), human very-low-density and low-density lipoproteins (catalog no ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein A-Sepharose (BioVision #6501-5) and rabbit polyclonal antibodies against Orc2 subunit were added and incubated with supernatant overnight ...
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bioRxiv - Cancer Biology 2021Quote: EZClick global protein synthesis kit (Biovision) was used to detect nascent protein synthesis following manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and protein concentration in the supernatant was determined using BCA protein assay Kit (Ref#K812-1000, Biovision). Each protein sample (45 µg ...
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bioRxiv - Cancer Biology 2020Quote: The plasma membrane protein extraction kit (BioVision) was used to separate the plasma membrane fraction from other cellular membranes according to the manufacturer’s recommended protocol ...
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bioRxiv - Pathology 2022Quote: ... The results were normalized to the total protein for associated samples measured by a BCA protein assay (BioVision, K813). For EV or matrix vesicle TNAP activity measurement ...
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bioRxiv - Plant Biology 2023Quote: ... GST-tag Protein Purification Kit (Glutathione Sepharose, BioVision) and MBP-tag Protein Purification Kit (Dextrin Beads ...
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bioRxiv - Systems Biology 2023Quote: ... the nuclear and cytoplasmic protein extraction kit (Biovision Inc. ...
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bioRxiv - Biophysics 2019Quote: Biotinylation of plasma membrane proteins was performed using a slightly modified protocol from a commercially available cell surface protein isolation kit (BioVision, Milpitas, CA USA). CHO-K1 cells were first transfected with ASIC3-TUR or ASIC1a-CER with and without unlabeled STOM ...
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bioRxiv - Biochemistry 2022Quote: ... Protein phosphotyrosyl phosphatase (PTP) activity was measured in kidney lysates using fluorometric Protein Tyrosine Phosphatase Activity Assay Kit (#K829, BioVision Inc., Milpitas CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Upon addition of 20 μl protein A Sepharose beads (Biovision), samples were incubated on a shaker for 1 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The EZClick™ Palmitoylated Protein Assay Kit (BioVision, K416-100) was used in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... protein synthesis was inhibited using Anisomycin (60 µM; BioVision 1549). For protein synthesis stimulation ...
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bioRxiv - Biophysics 2022Quote: ... Cells were lysed with EZLys™ Bacterial Protein Extraction Reagent (BioVision) by following the manufacture’s method ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentrations were in quantified using s BCA assay kit (BioVision) and a BioTek Synergy plate reader and Gen5 software (BioTek Instruments ...
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bioRxiv - Immunology 2019Quote: ... coverslips were coated with 20 µg/ml Protein A (6500-10, BioVision) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Western blots were performed as previously described [19] using rabbit anti-human LDLR (1:500) (BioVision, Inc., Milpitas, CA), TAGLN (1:1000) ...
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bioRxiv - Molecular Biology 2019Quote: ... We followed the protocol provided for the Plasma Membrane Protein Extraction Kit (BioVision, CA) to extract the PM protein ...
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bioRxiv - Neuroscience 2020Quote: ... followed by overnight incubation with protein A/G agarose beads (Biovision, Mountain View, CA) on a shaking plate at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Activity of the purified protein was quantified by an amylase activity colorimetric kit (BioVision) utilizing the amylase-specific E-G7-pNP substrate (Fig ...
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bioRxiv - Cell Biology 2021Quote: ADP released into the supernatant from human platelets was quantified with an ADP ELISA Kit (Biovision ADP Colorimetric Assay Kit, #K355) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... 5–10 ug of GST fusion proteins were bound to glutathione sepharose beads (BioVision #6566) in Binding Buffer (PBS + 0.1 % NP-40 ...
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bioRxiv - Cell Biology 2023Quote: ... Extraction of cytoplasmic and nuclear proteins was performed using the Nuclear/Cytosol Fractionation Kit (BioVision) according to its instruction.
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bioRxiv - Cancer Biology 2020Quote: ... Protein synthesis evaluation by immunofluorescence was conducted following manufacturer protocol (K715-100, BioVision, San Francisco, CA). To examine effects of inhibiting the RUNX1-CBFβ interaction ...
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bioRxiv - Cell Biology 2020Quote: Surfaces were prepared by stamping 2 μg/ml of protein A/G (Biovision, San Francisco, CA) using PDMS stamps ...
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bioRxiv - Genetics 2020Quote: ... a 96-well plate was coated with enhanced GFP protein (BioVision, 4999-100, 5 µg/mL) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Proteins were detected using specific antibodies against ß-actin (3598R-100-BV, BioVision via BioCat, Heidelberg, Germany) or HCMV 45 kDa late antigen (MBS320051 ...
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bioRxiv - Cell Biology 2023Quote: ... glass coverslips were cleaned with ethanol and incubated with 20 μg/ml of protein A (BioVision, USA) for 1 hr at room temperature or 4°C over night ...