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Citations for Invivogen :
1 - 50 of 128 citations for Yeast Expression Cloning kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... that are compatible with cloning into the rhesus IgG1 pFUSE expression vectors (pFUSEss-CHIg-rhG1 and pFUSEss-CLIg-rhK from Invivogen; rhIgLC1*01 courtesy of D ...
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bioRxiv - Microbiology 2023Quote: IgA1 heavy chains were generated by cloning the variable regions of selected antibodies into the expression vector pFUSEss-CHIg-hA1 (Cat# pfusess-hcha1, Invivogen, San Diego, CA). Variable regions of the antibody coding sequences were PCR amplified using the IgG1 heavy chain expression plasmid as a template and custom primers that appended an EcoRI site and an NheI site at the 5’ and 3’ ends respectively ...
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bioRxiv - Bioengineering 2023Quote: ... were generated by Gibson cloning into pFUSE vector (InvivoGen). Fab genes were PCR amplified from the Fab phagemid and subcloned into pBL347 Fab expression vector ...
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bioRxiv - Immunology 2022Quote: ... were synthesized by Genscript and cloned into pINFUSE-mIgG2b-Fc2 expression plasmid (InvivoGen). Recombinant protein was produced by transient transfection of Expi293 cells and purified using HiTrap Protein A column ...
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bioRxiv - Immunology 2021Quote: Antibody variable regions were cloned into expression plasmids from Invivogen (#pfusess-hchg1 ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into the blasticidin selectable pUNO expression vector (Invivogen). Alpha-actinin-4 sstFRET522 was constructed by inserting the sstFRET module between 408aa and 409aa of human alpha-actinin-4 (XP_016882820.1) ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14). Wild-type human NIK and the NIK-K429/430A mutant plasmids were a gift from Prof ...
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bioRxiv - Biochemistry 2023Quote: ... Cas9 expression was induced with tetracycline at 1 μg/ml (InvivoGen) 24 h prior to transfection with an Amaxa nucleofector (Lonza ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Systems Biology 2022Quote: ... Salmonella typhimurium), fungal (Cryptococcus gattii, Candida albicans hyphae and yeast) and other relevant antigens (Poly:IC (100 ug/mL - Invivogen; TLR3 ligand), E ...
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bioRxiv - Molecular Biology 2021Quote: ... or on solid YPD-agar (1% yeast extract, 2% peptone, 2% D-glucose, 2% agar) and selected with 100 µg/ml Zeocin® (InvivoGen). For small scale expression screening ...
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bioRxiv - Genetics 2019Quote: ... For stable sgRNA and shRNA expression 3 days puromycin (2 μg/ml, Invivogen) selection was applied.
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bioRxiv - Cancer Biology 2023Quote: ... E2Crimson expression was maintained via 600 μg/mL Geneticin G418 (Invivogen, ant-gn-1), and 7.5% sodium bicarbonate (NaHCO3 ...
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Identification of SLC25A46 interaction interfaces with mitochondrial membrane fusogens Mfn2 and Opa1bioRxiv - Biochemistry 2023Quote: ... Plasmids were transformed into SMD1163 by electroporation and grown on YPDS (1% yeast extract, 2% peptone, 2% dextrose, 1 M sorbitol) zeocin (InvivoGen, ant-zn-1p) plates ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...
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bioRxiv - Immunology 2019Quote: ... into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines stably transducing expression constructs where supplemented with 100µg/mL of hygromycin (Invivogen), except for FGFR3-TACC3 which was supplemented with 800µg/mL of geneticin (Sigma-Aldrich).
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bioRxiv - Immunology 2021Quote: ... For gene expression analysis HL-60 cells were stimulated with 100 ng/ml LPS (InvivoGen) for 6 hours.
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bioRxiv - Biochemistry 2020Quote: ... were generated by inserting cDNA encoding the whole ectodomains of MT1-D273 or MT1N273 (cDNA encoding Met1-G543) into the multiple cloning site of pFUSE-rIgG-Fc1(InvivoGen). The cDNA fragment encoding sMT1-D273-Fc and sMT1-N273-Fc were then subcloned into pCEP4 vector (Thermo Fischer Scientific).
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Biochemistry 2021Quote: ... and the heavy chain and light chain fused to scFv of BiTE were generated by Gibson cloning into pFUSE (InvivoGen). Fabs were PCR-ed from the Fab phagemid and subcloned into pBL347 ...
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bioRxiv - Developmental Biology 2020Quote: ... The F7-Ab scFv sequence was cloned into the pFuse-hIgG1-Fc2 mammalian expression vector (Invivogen), containing the IL-2 signal sequence and the IgG1 crystallizable fragment (Fc) ...
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bioRxiv - Cancer Biology 2020Quote: MDA-MB-231 cells were stably transfected with 2 μg of pUNO1-hOSM expression construct (InvivoGen), using TurboFect™ followed by Blasticidin (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... partial mouse Azin1 expression plasmids were transfected using Lipofectamine 2000 in the presence of NATETM (InvivoGen) in some experiments.
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bioRxiv - Biochemistry 2020Quote: ... The amplified PCR products were digested with EcoRV/NicoI and subcloned into pFUSE_hIgG1_Fc2 mammalian expression vector (Invivogen), which contains a N-terminal IL2 secretion signal peptides (SP).
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bioRxiv - Cancer Biology 2020Quote: ... pUNO1-hSTING-HA3x (STING-HA) and pUNO1-hSTING-MRP (STING-DN) expression plasmids were purchased from Invivogen. STING-MRP (MITA-related protein ...
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bioRxiv - Immunology 2023Quote: ... and PRR expression was maintained by growing the cells in media containing Blasticidin (ant-bl-05, InvivoGen), Zeocin (ant-zn-05 ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc fusion proteins were generated by cloning the PSG1a cDNA (UniProt KB-P11464) and PSG9 cDNA (NM_002784) into the pFuse-IgG1e3-Fc1 vector (InvivoGen, San Diego, CA). The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen) ...
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bioRxiv - Molecular Biology 2021Quote: ... the zebrafish codon-optimized genes of the corresponding NIR FPs and mClover3 were cloned into an expression vector carrying the bidirectional promoter (p-EF1a:2xCMV:EF1a) consisting of two CpG free mCMV enhancers (Invivogen) each followed by CpG free hEF1alpha promoter (Invivogen ...
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bioRxiv - Molecular Biology 2021Quote: ... 1.5 µg of pcDNA5 construct were co-transfected with 1.5 µg Flippase expression vector (pOG44) and cells were selected with 100 µg ml-1 Hygromycin (InvivoGen). All cell lines generated and plasmids used in this study are listed in Supplementary Table 1.
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bioRxiv - Biophysics 2020Quote: Co-expression of FLAG- and HA-tagged UMOD isoforms was performed using a bicistronic vector pVITRO-hygro-mcs (Invivogen). For this purpose ...
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bioRxiv - Immunology 2022Quote: ... Freestyle™ 293-F were transfected with pUNO1-Spike-dfur expression vectors (Spike and SpikeV1 to V11 plasmids, Invivogen) (1.2 µg plasmid DNA per 106 cells ...
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bioRxiv - Molecular Biology 2021Quote: ... The M1 cells with stable CD9-EGFP expression was generated by transfection with pcDNA3.1 CD9-EGFP using Metafectene Pro (Biontex) and selection with G418 (InvivoGen). HEK293T cells were maintained in Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were gel purified and cloned into heavy and kappa expression plasmids52,53 adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK1 ampicillin resistant vectors (InvivoGen) using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... five different pseudotypes were generated using expression plasmids of respective spike variants: for prototype B.1/D614G as before (1) or sourced from Invivogen for VOCs Beta (Cat ...
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bioRxiv - Cell Biology 2020Quote: We generated dual expression barcoding vectors by inserting pairs of barcoding protein sequences into the pVITRO1-hygro-mcs plasmid (InvivoGen), which contains two multiple cloning sites MCS1 and MCS2 (Supplementary Fig ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2019Quote: ... KX571250-1324) were cloned into an expression plasmid adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human antibodies were expressed in HEK-293T cells following transient transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from Invivogen. Recombinant antibodies were purified using Protein A sepharose (IBA ...
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bioRxiv - Immunology 2021Quote: ... HEK-293T cells were transfected with the respective S protein expression plasmids for the delta variant (Cat. No. plv-spike-v8, Invivogen) and the omicron variant (A67V ...
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bioRxiv - Microbiology 2021Quote: Human embryonic kidney HEK293T/17-hACE2 cells with stable expression of human angiotensin-converting enzyme ACE2 (AXON Neuroscience SE) were prepared by stable transfection of pDUO2-hACE2-TMPRSS2a (InvivoGen) with transfection reagent Lipofectamine 3000 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... or 7.7G6 mAb were produced in HEK-293T cells following transfection with pairs of the IgG1 heavy and light chain expression plasmids according to protocols from InvivoGen. Human antibodies were purified from cell culture supernatants using Protein-A affinity chromatography ...
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bioRxiv - Biochemistry 2022Quote: ... K562s were transfected according to manufacturer’s protocol with EGFR using pre-packaged lentiviral particles (G&P Biosciences) and selected for EGFR expression by culture in 1 μg/mL puromycin (InVivoGen). K562s were transfected according to manufacturer’s protocol with CD20 using pre-packaged lentiviral particles (G&P Biosciences LTV-CD20 ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Immunology 2023Quote: ... cell culture supernatant was analyzed for NF-κB/AP-1-induced SEAP production by using QUANTI-Blue reagents and IL-8-dependent expression of Lucia luciferase using QUANTI-Luc reagents (both Invivogen). For QUANTI-Blue measurement ...
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bioRxiv - Immunology 2023Quote: ... These Gibson sequences are necessary for cloning of the VH and VL into our rabbit IgG1 expression vectors which are based on the pFUSE-rIgG-Fc (InvivoGen) vectors.75,76 For this second PCR ...
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bioRxiv - Microbiology 2022Quote: ... RIG-I stimulation was assessed by analyzing Lucia luciferase reporter gene expression in 20 µl cell culture supernatants using QUANTI-Luc™ (Invivogen). Data are expressed as the normalized fold change over mock infected cells ...
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bioRxiv - Immunology 2020Quote: The VRC01 LC-P2A-HC construct was PCR-amplified from engineered C57BL/6J mouse cDNA and cloned between the promoter and constant regions of a mouse IgG2b heavy chain expression vector (InvivoGen, #pfuse-mchg2b) using Gibson Assembly ...
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bioRxiv - Immunology 2020Quote: ... The coding sequences of the 4A3 heavy chain variable region and light chain variable region were amplified by adding the IL-2 signal peptide and cloned into the expression vectors pFUSE-CHIg-HG1 and pFUSE2-CLIg-hk (Invivogen, San Diego, CA), respectively ...