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Citations for Invivogen :
1 - 50 of 162 citations for SEAP ASSAY KIT since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... were transfected with the pNifty-SEAP reporter plasmid (Invivogen, #pnifty2-seap), containing NFκB transcription factor binding sites and a secreted alkaline phosphatase (SEAP ...
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bioRxiv - Immunology 2020Quote: ... We used pNiFty2-IFNB-SEAP and pNiFty2-56K-SEAP promoter-reporter plasmids (InvivoGen; San Diego, CA), encoding the INFβ minimal promoter and the ISG-56K promoter ...
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bioRxiv - Bioengineering 2022Quote: ... Secreted Embryonic Alkaline Phosphatase (SEAP) (Invivogen) were purchased for various cell processing and analysis experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... SEAP activity was detected using QuantiBlue (InvivoGen) and the absorbance quantified on a Tecan Infinite M1000 PRO microplate reader at 650 nm ...
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bioRxiv - Bioengineering 2020Quote: STAT5:SEAP reporter cells (HKB-il2, Invivogen) were maintained in DMEM supplemented with 4.5 g/L glucose ...
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bioRxiv - Cancer Biology 2021Quote: ... SEAP activity was detected using QuantiBlue (InvivoGen) and the absorbance quantified on a Tecan Infinite M1000 PRO microplate reader at 650 nm ...
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bioRxiv - Immunology 2022Quote: ... SEAP reporter 293 cells expressing hACE2 (Invivogen) were grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Bioengineering 2023Quote: RAW-Blue (NF-κB-SEAP) reporter cell line (Invivogen, raw-sp) was used to prove the proper incorporation of TLRas with the SNPs and their potencies compared to soluble TLRas ...
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bioRxiv - Microbiology 2022Quote: ... TLR9 activation was determined via SEAP levels determined by QuantiBlue™ (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Bioengineering 2021Quote: ... NF-kB-induced SEAP activity was detected using QUANTI-Blue™ (Invivogen) and by reading the OD at 650 nm ...
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bioRxiv - Immunology 2023Quote: The Raw-Blue (NF-kB-SEAP) reporter cell line (Invivogen, raw-sp) and THP-1 hTLR9 reporter cell line (Invivogen ...
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bioRxiv - Cancer Biology 2024Quote: ... SEAP activity was then quantified using Quanti-Blue solution (InvivoGen, rep-qbs) and absorbance was measured using FLUOstar Omega device (BMG Labtech).
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bioRxiv - Pathology 2023Quote: ... SEAP activity was determined at 655 nm according to the manufacturer’s recommendations (InvivoGen). All samples were measured in triplicate ...
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bioRxiv - Neuroscience 2021Quote: ... SEAP activity in the culture supernatants were measured with QUANTI-Blue™ Solution (Invivogen) following the manufacture’s protocolThe data were analyzed in comparison with control empty vector transfection after SEAP normalization ...
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bioRxiv - Immunology 2021Quote: ... supernatants were collected and quantification of SEAP was performed according to manufacturer’s instructions (InvivoGen).
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bioRxiv - Immunology 2022Quote: ... before addition of SEAP reporter 293 cells expressing hACE2 (Invivogen, cat. code hkb-hace2). Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP ...
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bioRxiv - Cancer Biology 2024Quote: The reporter cell line-THP1-Blue (TM) NF-kB SEAP reporter (Invivogen, Cat# thp-nfkb) derived from the human THP1 monocytes cell line was obtained ...
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bioRxiv - Immunology 2021Quote: ... SEAP amounts were then measured in the cell culture supernatants using Quanti-Blue Detection Media (InvivoGen) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per the manufacturer’s instructions using recombinant mouse IFN-β (PBL Assay Science ...
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bioRxiv - Immunology 2020Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
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bioRxiv - Microbiology 2021Quote: ... by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen), followed by absorbance measurement at 600 nm ...
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bioRxiv - Microbiology 2023Quote: ... and TLR9 activation was quantified by measuring secreted embryonic alkaline phosphatase (SEAP) by QuantiBlueTM reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Neuroscience 2022Quote: ... HEK-Blue™-hTLR2 cell line (Co-transfected with human TLR2 and SEAP reported gene) was purchased from InvivoGen. These cells were grown in DMEM media supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... NfkB activity was measured in the same supernatants using 20uL of supernatant with180uL QUANTI-Blue SEAP detection reagent (InvivoGen). The samples were then incubated at 37 °C for 1 h and absorbance was measured at 640 nm ...
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bioRxiv - Microbiology 2024Quote: ... Twenty microliters of supernatant collected from infected cells was added to 180 μl of the SEAP detection solution (InvivoGen), followed by incubation at 37°C for ∼6 h ...
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bioRxiv - Immunology 2021Quote: ... SEAP activity as induced by NF-kB activation was assessed by the addition of pre-warmed Quantiblue reagent (Invivogen, USA) followed by incubation for 24 h at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... supernatants from iBMDM infections were incubated with the reporter cell line and levels of SEAP in the supernatants were determined using the detection medium QUANTI-Blue (Invivogen) after 24 h as per manufacturer’s instructions using recombinant mouse IFNβ (PBL assay science ...
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bioRxiv - Pathology 2020Quote: ... The conditioned media from each treatment group was analyzed for the presence of SEAP using QUANTIBlue™ detection reagent (Invivogen) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: HEK293 stably transfected with an SEAP reporter (HEK-Dual and HEK-Blue) responsive to STAT1 (IFNγ/GAS) or IRF (ISG) were obtained from Invivogen. Cells were seeded in a 96 well plate and treated with IFNγ alone or in the presence of BCL6 inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... The secreted embryonic alkaline phosphatase (SEAP) was measured in a microplate reader (OD Ex/Em = 620/655 nm) using Quanti-Blue Solution (InvivoGen), following the manufacturer’s instructions.
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bioRxiv - Bioengineering 2023Quote: ... Cells were then pelleted (150 RCF, 5 min) and SEAP enriched supernatant was then utilized for detection media (QUANTI-Blue, Invivogen) activation.
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bioRxiv - Bioengineering 2023Quote: ... 50 μL cell supernatant was collected from each sample and added to 150 μL of QUANTI-Blue SEAP detection medium (InvivoGen) and incubated for 2 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... the luciferase detection reagent QUANTI-Luc 4 Lucia/Gaussia (Cat. #.: rep-qlc4lg1) and the SEAP detection reagent QUANTI-Blue 4 (Cat. #.: rep-qbs) were purchased from InvivoGen. Cell staining antibodies for the flow cytometry analysis ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Microbiology 2024Quote: ... NF-κB activity was monitored by the production of secreted embryonic alkaline phosphatase (SEAP) through the hydrolysis of HEK-Blue (InvivoGen). Optical density was read at 650 nm using a SpectraMax340 PC plate reader (Molecular Devices).
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured for 16 hours after which supernatant was harvested and SEAP reporter protein secretion was determined using QUANTI-Blue™ detection medium (Invivogen) and measuring absorption at 650 nm on a 550 Microplate reader (Bio-Rad ...
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bioRxiv - Synthetic Biology 2021Quote: SEAP was detected in the media and in the lysate with an alkaline phosphatase detection medium – powder QUANTI-Blue™ (InvivoGen) as per the products instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP) activity into cells supernatant using QUANTI-Blue™ Solution (Invivogen), according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... Twenty mL of HEK-Blue™ supernatants were transferred to a 96-well plate containing 180 mL pre-warmed (37°C) QUANTI-Blue™ (the substrate for SEAP, InvivoGen, rep-qbs). Reactions were developed at 37°C for 50-90 min and measured at 630 nm using FLUOstar Omega Microplate Reader (BMG Lactech) ...
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bioRxiv - Immunology 2023Quote: ... NF-κB activation was determined after 20 h of incubation according to the manufacturer’s instructions by mixing 20 μl of supernatant with 180 μl of SEAP detection reagent (Quanti-BlueTM, InvivoGen, San Diego, CA, USA). The plates were incubated for 2 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... NF-kB activity was measured by combining 20 μL of supernatant with180 μL QUANTI-Blue SEAP detection reagent (InvivoGen, cat no. rep-qbs) in 96-well assay plate ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Immunology 2020Quote: ... Levels of SEAP were detected in the culture media after 3 hours incubation of supernatants with Quanti-Blue solution (InvivoGen, San Diego, CA, USA) at 650nm wavelength by ELISA reader.
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bioRxiv - Immunology 2020Quote: ... Promoter induction was measured by the SEAP reporter secretion (quantified at 620nm following addition of Quanti-Blue™ media, Invivogen, per the manufacturer’s instructions).
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...