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Citations for Invivogen :
1 - 50 of 413 citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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bioRxiv - Microbiology 2023Quote: ... B.1.1.529/BA.1 SARS-CoV-2 spike plasmid (plv-spike-v11) was obtained from InvivoGen and subcloned into pcDNA3.1-puro ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Physiology 2023Quote: Mouse FGL1 cDNA sequences (full length, N-terminal domain, globular domain) were cloned into pFUSEN-hG2Fc plasmid (InvivoGen) with the following modifications ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Microbiology 2022Quote: ... of recombinant viruses or purified SARS-CoV-2 spike protein adjuvanted with aluminum hydroxide (alum, 2% alhydrogel adjuvant, Cat# vac-alu-250, InvivoGen, San Diego, CA, USA). A prime-boost vaccination regimen was used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43 or 15 µg of SARS-CoV spike protein in 150 µL PBS together with 50 µL Addavax (MF59-like squalene adjuvant from InvivoGen) through intraperitoneally injection (i.p.) ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Immunology 2022Quote: ... Mice in the RBD + aluminum hydroxide condition received 10 µg of recombinant monomeric SARS-CoV-2 RBD protein formulated with 100 µg of Alhydrogel adjuvant 2% (Invivogen). Mice in the PBS vaccination group received phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... were immunized with 3 ugs of purified RBD of SARS-CoV-2 mixed with 10 ugs of poly I:C (Invivogen) twice with 3 weeks interval (17 ...
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bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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bioRxiv - Neuroscience 2023Quote: ... with cMyc tag and 6xHis-tag at the nanobody’s C-terminal were cloned into a pFUSE-derived vector (InvivoGen). The vector was used to transform Expi293F mammalian cells (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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bioRxiv - Biophysics 2019Quote: The coding sequences of antibody Fc heterodimerization domains were amplified from the pFUSE-hIgG1-Fc1 plasmid (Invivogen) containing two charged mutations on each opposing domain57,58 ...
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bioRxiv - Immunology 2023Quote: Spike proteins (2 μg/dose) were formulated with Alhydrogel 2% (InvivoGen, San Diego, CA, USA) as previously described (20) ...
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bioRxiv - Biochemistry 2020Quote: ... 17 ECD-10His (extracellular domain) was generated by Gibson cloning and adapted from the commercially available pFUSE-hIgG1-Fc (InvivoGen) vector ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR9 (Invivogen hkb-htlr9), the corresponding parental HEK293 null1 (InvivoGen hkb-null1) ...
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bioRxiv - Microbiology 2023Quote: HEK293-hTLR9 (Invivogen hkb-htlr9) and the corresponding parental HEK293 null1 (InvivoGen hkb-null1 ...
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bioRxiv - Genomics 2020Quote: HEK293/hTLR4A-MD2-CD14 Cells (Invivogen) were selected for functional follow up of the IRF1 trans-eQTL because of the ease of transfection of HEK293 cells and the addition of the TLR4 receptor ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Immunology 2021Quote: ... or anti-spike-RBD- mIgG2a (InvivoGen) were diluted in blocking solution and used as standards for the assay ...
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bioRxiv - Immunology 2021Quote: ... Hek293/hTLR4-HA cells (InvivoGen#293-htlr4ha) were a gift from Guang Yang (Jinan University).
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bioRxiv - Microbiology 2022Quote: HEK293 (HEK-Blue™ reporter cells, InvivoGen) -IFN-α/β and -IFN-λ cells were cultivated in DMEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... pUNO1-SpikeV8 carrying delta variant spike and pUNO1-SpikeV11 carrying omicron (B.1.1.529/BA.1 lineage) spike were purchased from InvivoGen (catalogue numbers p1-spike-v8 and p1-spike-v11 respectively).
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bioRxiv - Immunology 2023Quote: ... Beta (B.1.351) (InvivoGEn, plv-spike-v3) and Delta (B.1.617.2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding parental HEK293 null1 (InvivoGen hkb-null1), HEK293-hTLR7 (Invivogen hkb-htlr7) ...
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bioRxiv - Immunology 2023Quote: HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
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bioRxiv - Immunology 2022Quote: ... Freestyle™ 293-F were transfected with pUNO1-Spike-dfur expression vectors (Spike and SpikeV1 to V11 plasmids, Invivogen) (1.2 µg plasmid DNA per 106 cells ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Immunology 2023Quote: ... and Delta (B.1.617.2) (InvivoGen, plv-spike-v8). Plasmids encoding the S protein from the Omicron variants (B.1.1.529 and BA.2 ...
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bioRxiv - Microbiology 2023Quote: ... and the corresponding parental HEK293 null1 (InvivoGen hkb-null1) cell lines were grown in DMEM media supplemented with 10% heat-inactivated FBS and 1X GlutaMAX (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 µg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 μg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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bioRxiv - Systems Biology 2020Quote: ... HEK293 or U2OS cells were treated with 10 μM MG132 (InvivoGen) at approximately 80% confluence for 4 h and successively harvested ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Immunology 2022Quote: ... Reporter IFNγ (GAS) and ISG (IRF) HEK293 cell lines were purchased from Invivogen and expanded in DMEM supplemented with 10% heat inactivated FBS and selection antibiotics per vendor instructions.
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bioRxiv - Immunology 2022Quote: Mice were preimmunized intraperitoneally with 200 μg of sheep IgG (Serotec) in Addavax (Invivogen), followed by intravenous injection of sheep nephrotoxic serum (2.5 μL of serum per gram of mouse ...