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Citations for Invivogen :
1 - 50 of 470 citations for SARS CoV 2 Spike Glycoprotein S2 aa 800 1000 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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bioRxiv - Microbiology 2023Quote: ... B.1.1.529/BA.1 SARS-CoV-2 spike plasmid (plv-spike-v11) was obtained from InvivoGen and subcloned into pcDNA3.1-puro ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Immunology 2021Quote: ... Mice were immunized with SARS-CoV-2 spike protein at 0.5 μg/50μl alum (InvivoGen, stock concentration aluminum 10 μg/μl)/mouse intraperitoneally on days 0 and 14 (Fig 1A) ...
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bioRxiv - Microbiology 2022Quote: ... of recombinant viruses or purified SARS-CoV-2 spike protein adjuvanted with aluminum hydroxide (alum, 2% alhydrogel adjuvant, Cat# vac-alu-250, InvivoGen, San Diego, CA, USA). A prime-boost vaccination regimen was used ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The expression vector containing SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The expression vector containing the SARS-CoV-2 full Spike wild-type (WT) gene from Wuhan-Hu-1 isolate (pUNO1-SARS-S) was purchased from InvivoGen (San Diego, CA). The pFB-Luc plasmid vector was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Microbiology 2020Quote: ... HCoV-OC43 or 15 µg of SARS-CoV spike protein in 150 µL PBS together with 50 µL Addavax (MF59-like squalene adjuvant from InvivoGen) through intraperitoneally injection (i.p.) ...
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bioRxiv - Immunology 2022Quote: ... Mice in the RBD + aluminum hydroxide condition received 10 µg of recombinant monomeric SARS-CoV-2 RBD protein formulated with 100 µg of Alhydrogel adjuvant 2% (Invivogen). Mice in the PBS vaccination group received phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2020Quote: ... LPS (from E. coli K12, Invivogen) was dissolved in RPMI and used at a final concentration of 100ng/mL ...
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bioRxiv - Microbiology 2021Quote: ... were immunized with 3 ugs of purified RBD of SARS-CoV-2 mixed with 10 ugs of poly I:C (Invivogen) twice with 3 weeks interval (17 ...
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bioRxiv - Microbiology 2021Quote: BALB/c mice were immunized with 10 µg of SARS-CoV-2 RBD adjuvanted with 50% AddaVax™ (InvivoGen), via intramuscular route (i.m.) ...
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Neuroscience 2020Quote: ... LPS (LPS from E. coli O111:B4) (Invivogen), polyinosinic:polycytidylic acid (Invivogen) ...
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bioRxiv - Immunology 2020Quote: ... The immunization mixture contained 13 μg of SARS-CoV-2-S-I53-50NPs (equal to 10 μg of SARS-CoV-2-S) adjuvanted with 50 μg of polyinosinic-polycytidylic acid (Poly-IC; Invivogen). Blood was collected at weeks-1,2 ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Immunology 2022Quote: ... Ultrapure LPS (E. coli 0111:B4) was purchased from Invivogen. MitoTEMPO and UK-5099 were purchased from Sigma Aldrich (SML0737 ...
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bioRxiv - Immunology 2021Quote: ... The plates were then incubated with sequentially 1:5 serially diluted hACE2-Fc (invivogen) and two anti-SARS-CoV-2 RBD mAbs (clones H4 [InvivoGen] and CR3022 [Abcam]) starting at 10 μg/ml in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... and LPS (100 ng / mL E. coli K12, k-eklps, Invivogen) with IFNγ (20 ng / mL ...
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bioRxiv - Immunology 2019Quote: ... or LPS (O111:B4, E. coli, 1µg/2,5×105 cells Invivogen) was achieved using FuGeneHD (Promega ...
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bioRxiv - Immunology 2023Quote: ... and LPS-EB (E. coli 0111:B4) were purchased from InvivoGen.
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg/ml LPS (Ultrapure LPS from E. coli, 0111:B4, Invivogen) and 4 μM total monomer of sonicated Aβ fibrils were loaded into the nanopipette ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Immunology 2022Quote: ... 2′3′-RR c-di-AMP (2′3′-RR-S2 CDA) (Invivogen cat. no. tlrl-nacda2r), 2′3′- cyclic-di-GMP-AMP (2′3′-cGAMP ...
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bioRxiv - Immunology 2023Quote: Spike proteins (2 μg/dose) were formulated with Alhydrogel 2% (InvivoGen, San Diego, CA, USA) as previously described (20) ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMCs were rested and stimulated with 100 ng/ml LPS (from E. coli K12, Invivogen) or 0.1 μM CpG 2006 (TIB MOLBIOL) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stimulated with ultrapure lipopolysaccaride (1 μg/mL, LPS, E. coli 0111:B4, InvivoGen), harvested and analyzed as described below at 0 ...
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bioRxiv - Molecular Biology 2020Quote: ... received from Havard Medical school) and lipopolysaccharides (LPS, 1 µg/mL, E. coli 0111:B4, InvivoGen), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were activated with LPS (100 ng mL-1 E. coli O55:B5, triple-purified, InvivoGen) for 48 h ...
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bioRxiv - Immunology 2020Quote: ... USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated ...
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bioRxiv - Neuroscience 2023Quote: ... microglia cells were first primed with 100 ng/ml ultrapure LPS (E. coli 0111:B4, Invivogen) and then incubated at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... cGAS derivatives (pUNO1-hcGAS-AA, Invivogen pUNO1-hcGAS-AA ...
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bioRxiv - Immunology 2020Quote: ... Mature-DC were induced by adding LPS (200 ng/ml; E. coli K12; Invivogen, San Diego, CA) in the last 18h of culture ...
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bioRxiv - Immunology 2021Quote: ... TLR4 activation was stimulated by adding lipopolysaccharide (LPS-EB from E. coli 0111:B4; InvivoGen, Toulouse, France) in a final concentration of 50 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... moDCs were stimulated with 100 ng/mL ultrapure LPS (E. coli 0111 B4 strain, InvivoGen, San Diego) or left untreated ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded at 2.5 × 105 cells/well in 24-well tissue-culture plates, treated with EtOH (1.69%, or 0.83%) 10 ng/mL LPS (Ultrapure LPS, E. coli 0111:B4, Invivogen), 500 μM or 1 mM palmitic acid (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... injection with10 mg/kg in a total volume of 100μl of LPS (E. coli 055:B5 ultrapure; Invivogen). Mice were culled after 90 mins and serum collected via cardiac puncture for measurement of serum cytokines.
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bioRxiv - Immunology 2023Quote: ... immature DCs were stimulated with 100 ng/mL ultrapure LPS (E. coli 0111 B4 strain, InvivoGen, San Diego). In some experiments ...
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bioRxiv - Immunology 2023Quote: ... was diluted in sterile endotoxin-free water. TLR3 agonist polyinosinic-polycytidylic acid - Poly(I:C) and TLR4 agonist LPS (Lipopolysaccharide from E. coli O55:B5) (Invivogen) were diluted in sterile endotoxin-free physiological water (NaCl 0.9%) ...
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bioRxiv - Immunology 2022Quote: ... Immature moDC (0.5×106/ml) were stimulated with 100 ng/ml LPS (E. coli 0111 B4 strain, InvivoGen, San Diego) on day 5-6 in the presence or absence of F ...
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bioRxiv - Neuroscience 2022Quote: ... some cells were activated with LPS (100 ng per mL, triple-purified from E. coli strain O55:B5, InvivoGen, Inc.) or IFNγ (20 ng per mL) ...
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bioRxiv - Immunology 2019Quote: ... LL37 was from InvivoGen (see Table S2) and DOTAP from Roth ...
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bioRxiv - Immunology 2021Quote: ... or anti-spike-RBD- mIgG2a (InvivoGen) were diluted in blocking solution and used as standards for the assay ...
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bioRxiv - Synthetic Biology 2020Quote: ... 800 μg/mL G418 (InvivoGen), 10 μg/mL blasticidin S (InvivoGen) ...