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Citations for Invivogen :
1 - 50 of 95 citations for Recombinant Mouse icos ligand His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... TLR2/TLR1 ligand Pam3CSK4 and TLR2/6 ligand FSL-1 were from InvivoGen: All LPS were resuspended in sterile PBS 1x.
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bioRxiv - Immunology 2022Quote: ... cDNA encoding His-tagged Spike and RBD variants of SARS-CoV-2 were sub-cloned into pFUSE2ss-CLIg-hk (InvivoGen). The vectors were transfected as described for above the ACE2-albumin fusion protein and secreted His-tagged proteins were purified on HisTrap™ HP 1 mL columns (Cytiva) ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... The TLR ligands FLS1 (Invivogen, France) and CRX (Invivogen ...
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bioRxiv - Cell Biology 2023Quote: ... TLR2 ligand Pam3csk4 (Invivogen, tlrl-pms) was coupled to activated beads at a final concentration of 100μg/ml for 3h at room temperature with gentle rocking ...
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bioRxiv - Immunology 2022Quote: ... TLR2-ligand Pam3CSK4 (Invivogen, San Diego, CA), Alamar-blue (Life technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The RIG-I specific ligand 3p-hpRNA and the MDA5/TLR3 ligand poly(I:C) HMW (High Molecular Weight) were from Invivogen.
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bioRxiv - Immunology 2020Quote: ... TLR4 ligand lipid A (1 μg/ml, Invivogen), TLR7 ligand R-848 (30 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... TLR7 ligand R-848 (30 ng/ml, Invivogen), TLR9 ligand ODN1826 (sequence 5’-TCCATGACGTTCCTGACGTT-3’ ...
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bioRxiv - Immunology 2020Quote: ... TLR1/2 ligand Pam3CSK4 (100 ng/ml, Invivogen), TLR4 ligand lipid A (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... treated with CM 1:1 diluted in fresh cell growth media or PC (positive control: 1 μg/ml TLR-2 ligand Pam3CSK4 and 100 nM TLR-9 ligand CpG ODN 1668, both InvivoGen, USA). For experiments with different glucose concentrations ...
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bioRxiv - Immunology 2020Quote: ... Toll ligands were purchased from Invivogen (San Diego, CA).
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bioRxiv - Immunology 2020Quote: ... EV or Toll-like receptor–ligands (TLR-L) (InvivoGen, Toulouse, France): imiquimod (IMIQ ...
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bioRxiv - Immunology 2019Quote: ... Cells were transfected with STING ligand (DMXAA, 10 μg/ml, InvivoGen) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... B cells were activated with TLR9 ligand CpG ODN 2006 (1μM, Invivogen), alongside IL-2 (25U/ml ...
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bioRxiv - Immunology 2023Quote: ... and 20 μg/dose CpG ODN 2395 – TLR9 ligand (InvivoGen, #tlrl-2395-5) in 100 μL of PBS (pH 7.4) ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen) and 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... then trained with the following ligands for 24 hours: zymosan (1 μg/mL, InvivoGen tlrl-zyn), ETSU β-glucan (10 μg/mL) ...
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bioRxiv - Immunology 2021Quote: ... cells were sorted in wells pre-seeded with human CD40 ligand-expressing MS40L cells (5,000 cells/well) and containing 2.5µg/ml CpG ODN2006 (tlrl-2006; InvivoGen), 5µM CHK2 kinase inhibitor II ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Immunology 2020Quote: Neutrophils isolated from 4 healthy volunteers were plated at 50,000 cells per well and stimulated in duplicate with the following ligands for 1 hour: lipotechoic acid (LTA) 100ng/mL (Invivogen), LPS (Sigma ...
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bioRxiv - Immunology 2021Quote: ... by staining and acquiring control samples consisting of aliquots of fixed and frozen blood leukocytes from a healthy macaque after ex vivo stimulation of whole blood for 2 hours with three TLR ligands: LPS (LPS E. coli 0111: B4, Invivogen) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Animals received 1 μg of the TLR-2 ligand Pam3Cys-Ser-(Lys)4 trihydrochloride (Pam3Cys) (Invivogen, San Diego, CA, USA), 1 μg of the TLR-4 ligand LPS (Sigma-Aldrich Corp. ...
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bioRxiv - Systems Biology 2022Quote: ... Salmonella typhimurium), fungal (Cryptococcus gattii, Candida albicans hyphae and yeast) and other relevant antigens (Poly:IC (100 ug/mL - Invivogen; TLR3 ligand), E ...
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bioRxiv - Immunology 2020Quote: ... animals received naked plasmid DNA or isolated recombinant proteins (30 μg/animal of designated recombinant protein, coupled with/without alum adjuvant (40 μg/animal) Alhydrogel® (Invivogen; vac-alu-250).
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bioRxiv - Microbiology 2020Quote: ... prepared from recombinant flagellin from Salmonella typhymurium (RecFLA-ST, Invivogen) in sterile water was also added to the plate in triplicate ...
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bioRxiv - Immunology 2022Quote: ... 40µg recombinant RBD plus 5µg LPS-EB VacciGrade™ (InvivoGen) was given to isoflurane anesthetized mice in sterile PBS (50µL ...
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bioRxiv - Immunology 2021Quote: 1×106 freshly thawed PBMC or 1×105 purified CD14+ monocytes were cultured in RPMI supplemented with 10% FBS with or without 100 ng/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) for 16 or 6 hours ...
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bioRxiv - Immunology 2020Quote: ... 106 PBMC were stimulated for 16h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA); Brefeldin A (Sigma ...
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bioRxiv - Immunology 2020Quote: 105 purified monocytes were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) in 96-well tissue culture plates at 37C in a 5% CO2 environment for 8 hours ...
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bioRxiv - Immunology 2022Quote: ... 106 thawed cells were stimulated for 6h at 37°C in RPMI supplemented with 10% FBS in the presence or absence of bacterial TLR cocktail containing 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego, CA), 2 ug/mL Pam3CSK4 (TLR1/2 agonist ...
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bioRxiv - Immunology 2023Quote: 1×106 freshly thawed PBMC were cultured in RPMI supplemented with 10% FBS with or without 1 ug/mL LPS (TLR4 ligand, E.coli 055:B5; Invivogen, San Diego CA) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Immunology 2022Quote: ... 106 UCBMC were stimulated for 16h at 37°C in RPMI 1640 medium supplemented with 10% FBS in the presence or absence of 1 ug/mL LPS (TLR4 ligand, E. Coli 055:B5; Invivogen, San Diego CA). Brefeldin A (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Immunology 2022Quote: The following recombinant proteins were purchased and mixed with adjuvant (AddaVax, Invivogen) before injection into mice.
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bioRxiv - Biophysics 2020Quote: Co-expression of FLAG- and HA-tagged UMOD isoforms was performed using a bicistronic vector pVITRO-hygro-mcs (Invivogen). For this purpose ...
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bioRxiv - Immunology 2023Quote: ... and S-29 groups were immunized with 15 μg of recombinant protein with AddaVaxTM (Invivogen) as adjuvant in the hock[49] ...
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: Wild-type and mutants MmEsco2-myc/his and H2B-mCherry were cloned into the pVITRO2-hygro-mcs vector (InvivoGen) in two steps ...
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...
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bioRxiv - Microbiology 2021Quote: ... the mouse received a final boost intraperitoneally with 100 μg of recombinant N6 protein of A/duck/Zhejiang/D9/2013 (H4N6) adjuvanted with 10 μg of poly (I:C) (Invivogen). Three days after the final boost ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Immunology 2023Quote: ... Pam3CSK4 (100 µg/mouse; InvivoGen), or Poly(I:C ...
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bioRxiv - Cell Biology 2023Quote: ... of the EVs and ENPs from EO771 m/p-mCherry or controls (medium alone, recombinant mCherry, LPS-EB ultrapure 10 µg/ml (Invivogen) and CpG 2 µg/ml (TriLink ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...