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Citations for Invivogen :
1 - 50 of 145 citations for Recombinant Human CD14 Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Human CD14+ monocytes (2×106/ml) were pre-incubated with human anti-Dectin-1 (10μg/ml, Invivogen), anti-TLR2 blocking antibodies (10μg/ml ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Recombinant human TNF-α was purchased from InvivoGen. The antiretroviral drugs efavirenz ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Microbiology 2020Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added in order to generate a standard curve for each assay ...
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bioRxiv - Immunology 2022Quote: ... serial dilutions of recombinant human IL-1β (Invivogen, San Diego, CA) were added to generate a standard curve for each assay ...
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bioRxiv - Genomics 2024Quote: ... or 30 ng/ml human recombinant TNF-α (rcyc-htnfa; Invivogen), respectively ...
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bioRxiv - Genomics 2020Quote: HEK293/hTLR4A-MD2-CD14 Cells (Invivogen) were selected for functional follow up of the IRF1 trans-eQTL because of the ease of transfection of HEK293 cells and the addition of the TLR4 receptor ...
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bioRxiv - Immunology 2024Quote: ... and heavy and light chain variable sequences were synthesized as single chain variable fragments (ScFvs) containing a interchain (Light-Heavy) G4S3 linker and cloned into a pFuse-hG1-Fc2 Fc-tagged vector (InVivoGen, San Diego, CA), in-frame between the vector supplied signal sequence (from the human Il-2 protein ...
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bioRxiv - Immunology 2020Quote: THP1-XBlue-CD14 reporter cells (InvivoGen, San Diego, USA) were seeded in 96 well plates in phenol red RPMI ...
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bioRxiv - Microbiology 2021Quote: THP1-XBlue-CD14 reporter cells (InvivoGen, San Diego, USA) were seeded in 96 well plates (180,000 cells/well ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Genomics 2024Quote: ... cells were stimulated for 45 minutes with 30 ng/ml human recombinant IL-1α (rcyec-hil1b; Invivogen) or 30 ng/ml human recombinant TNF-α (rcyc-htnfa ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Immunology 2023Quote: ... Dried lipid film was resuspended in 500 μL of buffer containing 25 mM Tris-HCl (pH 8.0) - 150 mM NaCl (Lp buffer) and 2 µg of recombinant human mIL-1β (Invivogen) or 10 µg of LDH (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Immunology 2022Quote: HEK-293 cells stably expressing TLR4/MD2/CD14 were purchased from Invivogen. Cells were seeded at 3.5 × 104 live cell/well in 96 well plates overnight and were transfected with NF-κB-luciferase reporter plasmid (50 ng/well ...
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bioRxiv - Immunology 2021Quote: ... CD14+ cells were cultured o/n with 100 ng/mL LPS (Invivogen) and treated with 300 nM JQ1(- ...
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bioRxiv - Immunology 2023Quote: ... for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D) complexed with Lyovec (Invivogen). Activation was carried out for 18 h unless stated differently ...
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bioRxiv - Systems Biology 2020Quote: ... HEK 293 stable TLR4-MD2-CD14 cell line was also purchased from InvivoGen. All chemicals were HPLC-grade unless specifically indicated ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... HFF expressing FLAG-tagged PML isoforms or mCherry-tagged PML were cultured in the presence of 500 μg/ml geneticin (InvivoGen) and 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... These analyses were conducted using either a HEK293T TLR4-CD14-MD2 IL-18 reporter line (Invivogen) or the RAW-Blue Cell assay (Invivogen) ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-8xHis-AviTag plasmids were generated by subcloning the Spike-RBD or ACE2 DNA fragment into a pFUSE-hIgG1-Fc-AviTag vector (adapted from the pFUSE-hIgG1-Fc vector from InvivoGen). The ACE2-Fc-LgBiT fusion plasmids were generated by subcloning the gene fragments of LgBiT to the N- or C-terminus of the ACE2-TEV-Fc-AviTag vector with a 10-amino acid (N-terminal fusion ...
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bioRxiv - Bioengineering 2020Quote: ... VH-Fc were cloned into a pFUSE (InvivoGen) vector with a human IgG1 Fc domain ...
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bioRxiv - Immunology 2023Quote: ... 10% FCS and 250µg/ml Hygromycin B gold (InvivoGen). Virus stock production was performed under BSL-3 conditions using DMEM medium supplemented with glutamax and 2% FCS ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Immunology 2020Quote: ... + 1% FCS and treated with 500 ng/mL Pam3CSK4 (InvivoGen) for 4 hours and 10 μM nigericin (InvivoGen ...
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bioRxiv - Pathology 2020Quote: ... devoid of TLR4) and HEK Blue™ TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines (Invivogen). Cells were subject to treatment for 24 hrs with rHSP27 (1 μg/mL) ...
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bioRxiv - Immunology 2020Quote: ... animals received naked plasmid DNA or isolated recombinant proteins (30 μg/animal of designated recombinant protein, coupled with/without alum adjuvant (40 μg/animal) Alhydrogel® (Invivogen; vac-alu-250).
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bioRxiv - Microbiology 2020Quote: ... prepared from recombinant flagellin from Salmonella typhymurium (RecFLA-ST, Invivogen) in sterile water was also added to the plate in triplicate ...
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bioRxiv - Immunology 2022Quote: ... 40µg recombinant RBD plus 5µg LPS-EB VacciGrade™ (InvivoGen) was given to isoflurane anesthetized mice in sterile PBS (50µL ...
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bioRxiv - Immunology 2023Quote: NF-κB/AP-1 activation in THP-1-XBlue-CD14 reporter monocytes was determined after 20– 24 h of incubation according to the manufacturer’s protocol (InvivoGen, San Diego, USA). Briefly ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen). For the generation of full length PSG1-Fc and PSG9-Fc ...
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bioRxiv - Pathology 2020Quote: HEK-Blue™ Null1-v and HEK-Blue™-TLR4 (stably expressing TLR4, MD-2 and CD14 co-receptor genes) cell lines were obtained from Invivogen (SanDiego, CA, USA). HEK-Blue™ SR-AI and HEK-Blue™ CD36 were prepared by stably transfecting the parental cell line HEK-Blue™ Null1-v with plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc. ...
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bioRxiv - Physiology 2022Quote: ... aeruginosa flagellin (FLA-PA) and soluble TLR5 ectodomain (hTLR5-Fc) were from InvivoGen. Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: ... the cells are resuspended in FACS buffer containing 5ng/μL Fc-hDectin1 (Invivogen) and incubated in the dark on ice for 1 hour ...
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bioRxiv - Immunology 2022Quote: The following recombinant proteins were purchased and mixed with adjuvant (AddaVax, Invivogen) before injection into mice.
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bioRxiv - Immunology 2022Quote: ... and human IL-1β (Invivogen), were dissolved in PBS and added to the cell culture medium to achieve final concentrations of 10 μM (ADP-heptose) ...
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bioRxiv - Immunology 2023Quote: ... human p21 (Invivogen; 100 ng), human p27 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...