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Citations for Invivogen :
1 - 50 of 134 citations for Rabbit Anti Sheep IgG H+L Alkaline Phosphatase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secreted Embryonic Alkaline Phosphatase (SEAP) (Invivogen) were purchased for various cell processing and analysis experiments ...
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bioRxiv - Immunology 2020Quote: ... The supernatants were discarded before staining with 50 μL of 2 μg/mL secondary AF488 goat anti rabbit (goat IgG H+L, Invivogen) in cytometry buffer for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... 20 µL of cell culture supernatant was then withdrawn and analyzed for alkaline phosphatase content via change in Quanti-Blue (Invivogen, rep-qbs) absorbance at 620 nm (Spectramax id3 plate reader).
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bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
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bioRxiv - Microbiology 2023Quote: ... and TLR9 activation was quantified by measuring secreted embryonic alkaline phosphatase (SEAP) by QuantiBlueTM reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Immunology 2022Quote: Mice were preimmunized intraperitoneally with 200 μg of sheep IgG (Serotec) in Addavax (Invivogen), followed by intravenous injection of sheep nephrotoxic serum (2.5 μL of serum per gram of mouse ...
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into pFUSEss H and L vectors (Invivogen) using NEB HIFI assembly kit (NEB ...
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bioRxiv - Biochemistry 2019Quote: ... Supernatant from HEK-Blue IFN-α/β cells was then assayed for alkaline phosphatase activity using QUANTI-Blue (InvivoGen), following manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... The secreted embryonic alkaline phosphatase (SEAP) was measured in a microplate reader (OD Ex/Em = 620/655 nm) using Quanti-Blue Solution (InvivoGen), following the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Microbiology 2024Quote: ... NF-κB activity was monitored by the production of secreted embryonic alkaline phosphatase (SEAP) through the hydrolysis of HEK-Blue (InvivoGen). Optical density was read at 650 nm using a SpectraMax340 PC plate reader (Molecular Devices).
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Synthetic Biology 2021Quote: SEAP was detected in the media and in the lysate with an alkaline phosphatase detection medium – powder QUANTI-Blue™ (InvivoGen) as per the products instructions ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP) activity into cells supernatant using QUANTI-Blue™ Solution (Invivogen), according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... anti-hIL-6-IgG (Invivogen) or mouse IgG1 kappa Isotype Control (eBioscience ...
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Immunology 2023Quote: ... and another 1 μg/ml of anti-hIL-6-IgG (Invivogen) or mouse IgG1 kappa Isotype Control were added to the wells after 2 days of co-culture.
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Targets for inhibition were TLR4 (anti-TLR4 IgG, 1 μg/ml; Invivogen); interleukin-1 receptor-associated kinase 1 and 4 (IRAK 1/4 ...
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bioRxiv - Immunology 2023Quote: ... 5µM H-151 (Invivogen) or 50 nM Bafilomycin A1 (Invivogen) ...
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bioRxiv - Neuroscience 2023Quote: ... Rat neutralizing monoclonal anti-mDECTIN-1-IgG (clone R1-4E4) (Invivogen mabg-mdect, 1:30); Rat monoclonal anti-CD206 (clone MR5D3 ...
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bioRxiv - Immunology 2020Quote: ... H-151 and BX795 (Invivogen) were diluted in DMSO ...
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bioRxiv - Immunology 2020Quote: ... STING inhibitor H-151 (2.5μM, Invivogen) or DMSO (vehicle control ...
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bioRxiv - Cell Biology 2021Quote: ... and 24 h later puromycin (InvivoGen) was used to select clones for two weeks ...
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bioRxiv - Neuroscience 2021Quote: ... dexamethasone (Invivogen, 10 μM, 24 h).
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bioRxiv - Cancer Biology 2020Quote: ... and STING antagonist H-151 (Invivogen) were dosed at 500 μg/gm of body weight i.p ...
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bioRxiv - Immunology 2021Quote: ... IgG4 using IgG plasmids (Invivogen) by conventional cloning techniques as previously described (35 ...
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bioRxiv - Neuroscience 2022Quote: ... puromycin (1 mg/L, Invivogen) was added to each media change the following two days ...
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bioRxiv - Immunology 2022Quote: H-151 (STING Inhibitor InvivoGen #inh-h151), GSK583 (RIPK2 inhibitor-Cayman Chemical Company #19739) ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Immunology 2022Quote: ... shControl and shLRBA HeLa cells were seeded at 0.3*106 cells in 6 well-plates followed by treatment for 16 h or 24 h with 5 μM of MG132 (InvivoGen) alone or in combination with 100 nM of Bafilomycin A1 for 3 h ...
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bioRxiv - Cancer Biology 2023Quote: ... In some cases, cells were first pre-treated for 1 h with following inhibitors (2 µM H-151, 10 µM MCC950 (InvivoGen)) before addition of the treatments ...
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bioRxiv - Neuroscience 2023Quote: ... They were incubated overnight in blocking buffer solution with the primary Anti-mDectin-1-IgG antibody (1:50; cat# mabg-mdect, Invivogen) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... For the H-151 STING inhibitor (Invivogen #inh-h151), macrophages were treated with vehicle (DMSO ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Immunology 2022Quote: ... normal rat PAb IgG control blocking antibodies (InvivoGen), Dexamethasone (10µM ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with L-Glutamine and Primocin (100mg/ml; InvivoGen) and maintained at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... and then 0.2g/L Neomycin/G418 (InvivoGen, San Diego, CA) selection media was added ...
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bioRxiv - Immunology 2020Quote: ... EV or Toll-like receptor–ligands (TLR-L) (InvivoGen, Toulouse, France): imiquimod (IMIQ ...
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bioRxiv - Immunology 2021Quote: ... Primary monocytes were then treated for 1 h 30 with nigericin (5 μM, Invivogen); UCN-01 (12.5 μM ...
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bioRxiv - Immunology 2020Quote: ... the cells were primed for 4 h with 500 ng/mL ultrapure LPS (InvivoGen). The supernatant was discharged and macrophages were infected with trypomastigote forms of T ...
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bioRxiv - Immunology 2024Quote: ... LPS (cat.#: tlrl-3pelps) and H-151 (cat.# inh-h151) were purchased from InvivoGen. Recombinant K48 ...
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bioRxiv - Cancer Biology 2023Quote: ... We added 50mg/L Plasmocin™ prophylactic (Invivogen, San Diego, CA, USA) to both DMEM and RPMI media ...
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bioRxiv - Microbiology 2023Quote: ... Penicillin/Streptomycin and 2mM L-Glutamine and 100 µg/ml primocin (Invivogen). The cells were harvested after 3-4 days when full cytopathic effect was observed ...