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Citations for Invivogen :
1 - 50 of 1094 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2019Quote: ... followed by treatment with either the mitogen-activated protein kinase kinases 1 and 2 (MEK1/2) inhibitor PD98059 (50 μM; InvivoGen, San Diego, CA, USA) to block ERK1/2 activation ...
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bioRxiv - Immunology 2023Quote: ... a potent inhibitor of the noncanonical IkB kinases TANK-binding kinase-1 (TBK1) and IκB kinase-ε (IKKε) (Invivogen) were diluted in DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... TANK-binding kinase 1 (TBK1) and IκB kinase ε (IKKε; BX795, 2 μM; Invivogen); receptor for advanced glycation end products (RAGE ...
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bioRxiv - Genetics 2020Quote: ... Cells were lysed using passive lysis buffer and the activity of the enhancer assessed using a Lucia QUANTI-Luc Gold Assay #3 luciferase kit (InvivoGen). Because of clear evidence of the response of the pGL4.74 Renilla normalisation control plasmid to PMA treatments and co-transfection with the EGR1 expression vector Lucia activity levels were normalised against quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... These reporter proteins were measured in cell culture supernatants using QUANTI-Luc™ (IRF activity) and QUANTI-Blue™ (NF-κB activity) assays as described by the manufacturer (Invivogen).
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bioRxiv - Pharmacology and Toxicology 2023Quote: PLpro inhibitor antiviral activities were tested in a cell culture assay with A549:hACE2 cells (Invivogen) by the following protocol ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and secreted Luciferase activity assayed using QUANTI-Luc™ kit (InVivoGen) as per manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... secreted embryonic alkaline phosphatase activity was determined in the culture supernatants using the QUANTI-Blue assay following the protocol provided by manufacturer (InvivoGen). Lipopolysaccharides (LPS ...
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bioRxiv - Immunology 2021Quote: ... or 10mg/ml PI3 Kinase inhibitor (LY294002, Invivogen) as controls ...
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bioRxiv - Immunology 2020Quote: ... were harvested and incubated in 96-well plate (1×104 cells per well) with 250 μg/mL EndoFit Ovalbumin protein (InvivoGen) or 10 μg/mL OVA323–339 (ISQAVHAAHAEINEAGR ...
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bioRxiv - Cancer Biology 2021Quote: ... SEAP activity was detected using QuantiBlue (InvivoGen) and the absorbance quantified on a Tecan Infinite M1000 PRO microplate reader at 650 nm ...
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bioRxiv - Microbiology 2022Quote: ... Activity is determined using QUANTI-BlueTM (Invivogen), with a change from pink to purple-blue colour indicating a positive reaction and thus TLR5 induction ...
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bioRxiv - Cancer Biology 2021Quote: ... SEAP activity was detected using QuantiBlue (InvivoGen) and the absorbance quantified on a Tecan Infinite M1000 PRO microplate reader at 650 nm ...
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bioRxiv - Microbiology 2020Quote: ... IFN-β promoter activity was induced by transfecting 250 ng of poly I:C (average size 0.2-1 kb) (InvivoGen) during 16 h ...
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bioRxiv - Immunology 2020Quote: ... 5μg of PR8-HA protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink was injected into the left quadriceps and right gastrocnemius (50 μL per site) ...
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bioRxiv - Molecular Biology 2023Quote: ... RAW-Blue ISG and Quantified-Blue assay kit were obtained from Invivogen, Ltd ...
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bioRxiv - Biochemistry 2023Quote: ... luciferase activity was measured using the Quanti-Luc system (Invivogen).
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bioRxiv - Immunology 2021Quote: ... Protein vaccines were administered with 1:10 AdjuPhos (Invivogen).
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bioRxiv - Bioengineering 2023Quote: ... and the QUANTI-Blue™ assay kit were obtained from Invivogen (San Diego, CA). BrightGLO™ was purchased from Promega (Madison ...
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bioRxiv - Bioengineering 2023Quote: ... 20 µL of culture media was transferred to a 96-well clear-bottom black plate and 50 µL of QUANTI-Luc™ assay solution (Catalog Code: repqlc; Invivogen) was added directly to each well ...
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bioRxiv - Bioengineering 2021Quote: ... NF-kB-induced SEAP activity was detected using QUANTI-Blue™ (Invivogen) and by reading the OD at 650 nm ...
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bioRxiv - Cancer Biology 2024Quote: ... SEAP activity was then quantified using Quanti-Blue solution (InvivoGen, rep-qbs) and absorbance was measured using FLUOstar Omega device (BMG Labtech).
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bioRxiv - Molecular Biology 2020Quote: ... Protein translation was inhibited with 50 µg·mL-1 of puromycin (ant-pr-1, Invivogen).
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bioRxiv - Microbiology 2022Quote: ... the HEK293T cells were transfected with 30 µg of expression plasmid encoding the SARS-CoV-2 Wuhan-Hu-1 S protein (pCAG3.1/SARS2-Sd19) or Delta variant S protein (pUNO1-SpikeV8; Invivogen) using FuGENE HD transfection reagent (Promega ...
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bioRxiv - Pathology 2023Quote: ... SEAP activity was determined at 655 nm according to the manufacturer’s recommendations (InvivoGen). All samples were measured in triplicate ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 µg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2023Quote: ... mice were injected with equal amounts of SARS-CoV-2 protein delivered as 1) soluble protein in conjugation with adjuvant (Alhydrogel, 100 μg/dose, vac-alu-250, InvivoGen), or 2 ...
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bioRxiv - Immunology 2022Quote: ... The protein antigen was adjuvanted with AddaVax (1:1 v/v; vac-adx-10, InvivoGen, USA) for immunisation.
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bioRxiv - Immunology 2021Quote: WT and NLRP3-/- iBMDMs were seeded on 150 mm plates at 5×106 cells/plate and on the next day treated with 1 μg/ml LPS (Invivogen, Cat. no: tlrl-b5lps) for 3.5 h. ...
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bioRxiv - Biochemistry 2023Quote: Jurkat-Lucia TCR-hPD-1 effector cells and Raji-APC-hPD-L1 antigen presenting cells were obtained from Invivogen as components of the PD-1/PD-L1 Bio-IC assay (Invivogen, #rajkt-hpd1). The cells were cultivated in IMDM with glutamine and HEPES (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... SEAP activity in the culture supernatants were measured with QUANTI-Blue™ Solution (Invivogen) following the manufacture’s protocolThe data were analyzed in comparison with control empty vector transfection after SEAP normalization ...
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bioRxiv - Immunology 2020Quote: OT-1 splenocytes were activated with 20 μg/ml OVA protein (Invivogen) and 100 U/ml hIL-2 (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... 1 mg/mL of whole OVA protein (Invivogen, San Diego, CA, USA) or γ-irradiated and CFSE stained B16F10 melanoma cells were added to the culture ...
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bioRxiv - Immunology 2020Quote: ... RBD or OVA proteins were formulated in PBS at a 1:1 ratio with Addavax adjuvant (InvivoGen). C57BL/6 or BALB/c mice were anesthetised by isoflurane inhalation prior to intramuscular injection of 50 μL vaccine in each hind quadriceps ...
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bioRxiv - Cancer Biology 2019Quote: Retrovirus was generated by plating PlatE cells for 1 week in complete DMEM with the addition of selection antibiotics including 1 µg/mL puromycin (InvivoGen) and 10 µg/mL blasticidin (InvivoGen) ...
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bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
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bioRxiv - Immunology 2020Quote: ... right and left axillary LNs) were evaluated 14 days-post IM vaccination with PR8-HA (5μg) protein with Addavax (1:1 ratio; InvivoGen) and 0.5% tattoo ink ...
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bioRxiv - Immunology 2020Quote: ... we added the Syk-selective tyrosine kinase inhibitor piceatannol (at 10 or 30 μM) (Invivogen, San Diego, CA, catalog # tlrl-pct) or the NADPH oxidase inhibitor diphenyleneiodonium chloride (DPI ...
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bioRxiv - Synthetic Biology 2024Quote: ... cells were harvested from the 24-well plate when confluent by trypsinizing and transferring to a single well of a 12-well plate in 1 mL of medium supplemented with 1 μg/mL puromycin (Invivogen ant-pr) or 200 μg/mL hygromycin (Millipore ...
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bioRxiv - Immunology 2021Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Microbiology 2022Quote: ... BALB/c mice were vaccinated via the intramuscular route with 3 μg of each respective protein with 1:1 mixture of Addavax (Invivogen) in a total volume of 50 μL ...
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bioRxiv - Immunology 2023Quote: The agonistic activity of anti-CD40 monoclonal antibodies was tested by stimulating HEK-Blue-CD40L™ (Invivogen) cells which harbor an NF-κB-inducible Secreted Embryonic Alkaline Phosphatase (SEAP ...
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bioRxiv - Cancer Biology 2023Quote: IL-1R signaling activity was measured using HEK-BlueTM IL-1R cells (Invivogen, cat. code hkb-il1r) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The action of MAPK Kinases (MEK1 and MEK2) was blocked by the use of a selective inhibitor UO126 at 5 µM (Invivogen, CA, USA). The involvement of the TLR1/2 in signal transduction was tested using CU-CPT22 at 10 µM (Tocris Bioscience ...
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Promoter-adjacent DNA hypermethylation can downmodulate gene expression: TBX15 in the muscle lineagebioRxiv - Molecular Biology 2022Quote: ... Lucia and Cypridina luciferase activity was quantified by bioluminescence from aliquots of the cell supernatant (BioLux Cypridina Luciferase assay kit, New England Biolabs; Quanti-Luc, InvivoGen). Reference plasmid-normalized luciferase activity was from the average of three independent transfections ...
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bioRxiv - Immunology 2021Quote: ... or Quanti-Luc assay (InvivoGen).
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bioRxiv - Immunology 2022Quote: ... EndoFit chicken egg ovalbumin protein with endotoxin levels <1 EU/mg was purchased from Invivogen for in vivo use at a concentration of 200μg/mice or in vitro use at a concentration ranging from 1000 to 10μg/ml ...
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bioRxiv - Systems Biology 2021Quote: ... interferon regulatory factor (IRF)-activity was assayed using QUANTI-Luc™ luminescence reagent (InvivoGen, San Diego, CA, USA) and an INFINITE 200 PRO microplate reader (Tecan ...
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bioRxiv - Microbiology 2023Quote: ... Cells were monitored quarterly for retroviral contamination by SYBR-Green based PCR RT assay (43, 44) and tested for mycoplasma contamination by MycoStrip® detection kit (InvivoGen). Derivatives of HeLa and HT1080 cells containing doxycycline-inducible Mx2 ...
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bioRxiv - Pathology 2020Quote: ... FIV was diluted in PBS to 133μg/mL Coronavirus-specific protein and mixed 1:1 in 2% Alhydrogel (Invivogen vac-alu-250) to give a final concentration of 66.7μg/mL in 1% Alhydrogel ...