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Citations for Invivogen :
1 - 50 of 91 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S1-614D and S1-614G tagged with human IgG Fc fragment were constructed by insertion of the S1 region (residues 1-681) to pFUSE-hIgG1-Fc1 vector (InvivoGen, USA) and expressed using the Expi293 Expression system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR9 (Invivogen hkb-htlr9), the corresponding parental HEK293 null1 (InvivoGen hkb-null1) ...
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bioRxiv - Microbiology 2023Quote: HEK293-hTLR9 (Invivogen hkb-htlr9) and the corresponding parental HEK293 null1 (InvivoGen hkb-null1 ...
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bioRxiv - Genomics 2020Quote: HEK293/hTLR4A-MD2-CD14 Cells (Invivogen) were selected for functional follow up of the IRF1 trans-eQTL because of the ease of transfection of HEK293 cells and the addition of the TLR4 receptor ...
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bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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bioRxiv - Microbiology 2023Quote: ... B.1.1.529/BA.1 SARS-CoV-2 spike plasmid (plv-spike-v11) was obtained from InvivoGen and subcloned into pcDNA3.1-puro ...
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bioRxiv - Immunology 2021Quote: ... or anti-spike-RBD- mIgG2a (InvivoGen) were diluted in blocking solution and used as standards for the assay ...
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bioRxiv - Immunology 2022Quote: ... Omicron BA.1 or Omicron BA.2 Spike expression vectors lacking the 19 C-terminal amino acids containing the ER-retention signal (InvivoGen plv-spike-v11 and plv-spike-v12). HEK293T cells (1.2×106 cells ...
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bioRxiv - Immunology 2021Quote: ... Hek293/hTLR4-HA cells (InvivoGen#293-htlr4ha) were a gift from Guang Yang (Jinan University).
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bioRxiv - Microbiology 2022Quote: HEK293 (HEK-Blue™ reporter cells, InvivoGen) -IFN-α/β and -IFN-λ cells were cultivated in DMEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... pUNO1-SpikeV8 carrying delta variant spike and pUNO1-SpikeV11 carrying omicron (B.1.1.529/BA.1 lineage) spike were purchased from InvivoGen (catalogue numbers p1-spike-v8 and p1-spike-v11 respectively).
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bioRxiv - Immunology 2023Quote: ... Beta (B.1.351) (InvivoGEn, plv-spike-v3) and Delta (B.1.617.2 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding parental HEK293 null1 (InvivoGen hkb-null1), HEK293-hTLR7 (Invivogen hkb-htlr7) ...
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bioRxiv - Immunology 2023Quote: HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
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bioRxiv - Bioengineering 2022Quote: ... Human codon optimized Delta full-length SARS-CoV-2 Spike protein plasmid was synthesized by Invivogen (plv-spike-v8).
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bioRxiv - Immunology 2022Quote: ... Freestyle™ 293-F were transfected with pUNO1-Spike-dfur expression vectors (Spike and SpikeV1 to V11 plasmids, Invivogen) (1.2 µg plasmid DNA per 106 cells ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Immunology 2023Quote: ... and Delta (B.1.617.2) (InvivoGen, plv-spike-v8). Plasmids encoding the S protein from the Omicron variants (B.1.1.529 and BA.2 ...
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bioRxiv - Microbiology 2023Quote: ... and the corresponding parental HEK293 null1 (InvivoGen hkb-null1) cell lines were grown in DMEM media supplemented with 10% heat-inactivated FBS and 1X GlutaMAX (Life Technologies ...
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bioRxiv - Pathology 2020Quote: ... FIV was diluted in PBS to 133μg/mL Coronavirus-specific protein and mixed 1:1 in 2% Alhydrogel (Invivogen vac-alu-250) to give a final concentration of 66.7μg/mL in 1% Alhydrogel ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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bioRxiv - Systems Biology 2020Quote: ... HEK293 or U2OS cells were treated with 10 μM MG132 (InvivoGen) at approximately 80% confluence for 4 h and successively harvested ...
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bioRxiv - Neuroscience 2023Quote: ... with cMyc tag and 6xHis-tag at the nanobody’s C-terminal were cloned into a pFUSE-derived vector (InvivoGen). The vector was used to transform Expi293F mammalian cells (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Immunology 2022Quote: ... Reporter IFNγ (GAS) and ISG (IRF) HEK293 cell lines were purchased from Invivogen and expanded in DMEM supplemented with 10% heat inactivated FBS and selection antibiotics per vendor instructions.
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Microbiology 2021Quote: ... 20 µl HEK293 culture supernatant was added to 180 µl QUANTI-Blue reagent (InvivoGen) and incubated at 37°C for 4 hours ...
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bioRxiv - Bioengineering 2023Quote: ... A 19-mer 5’ppp-dsRNA positive control for RIG-I activation was obtained from InvivoGen (San Diego, CA; Catalog No. tlrl-3prna).
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bioRxiv - Biochemistry 2020Quote: ... ACE2-8xHis-AviTag plasmids were generated by subcloning the Spike-RBD or ACE2 DNA fragment into a pFUSE-hIgG1-Fc-AviTag vector (adapted from the pFUSE-hIgG1-Fc vector from InvivoGen). The ACE2-Fc-LgBiT fusion plasmids were generated by subcloning the gene fragments of LgBiT to the N- or C-terminus of the ACE2-TEV-Fc-AviTag vector with a 10-amino acid (N-terminal fusion ...
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bioRxiv - Bioengineering 2020Quote: ... VH-Fc were cloned into a pFUSE (InvivoGen) vector with a human IgG1 Fc domain ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: ... 10% FCS and 250µg/ml Hygromycin B gold (InvivoGen). Virus stock production was performed under BSL-3 conditions using DMEM medium supplemented with glutamax and 2% FCS ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Immunology 2022Quote: Inhibition of spike-mediated cell-cell fusion was tested using an assay developed by Invivogen. Briefly ...
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bioRxiv - Immunology 2023Quote: Spike proteins (2 μg/dose) were formulated with Alhydrogel 2% (InvivoGen, San Diego, CA, USA) as previously described (20) ...
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bioRxiv - Immunology 2022Quote: ... ALPK1 knockout and TIFA knockout HEK293 cells and their parental cell line were purchased from Invivogen and termed HEK293 cells ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Immunology 2020Quote: ... + 1% FCS and treated with 500 ng/mL Pam3CSK4 (InvivoGen) for 4 hours and 10 μM nigericin (InvivoGen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Microbiology 2020Quote: ... All HEK293 Flp-In T-REx cell lines were additionally supplemented with 5 µg/mL Blasticidin S (Invivogen) and 200 µg/mL Hygromycin B (Invivogen ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... boost 4 weeks later of 5 μg recombinant spike protein (aa 14-1209) in 1% alhydrogel (Invivogen) into the right hind limb ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen). For the generation of full length PSG1-Fc and PSG9-Fc ...
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Bioengineering 2021Quote: ... of SARS-CoV-2 spike protein (NCBI Accession: YP 009724390.1) were synthesized and cloned into the pFUSE expression vector (InvivoGen). Both constructs contained an AVI-tag and a 6xHis tag at the C-terminus separated by a single G4S linker ...
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bioRxiv - Physiology 2022Quote: ... aeruginosa flagellin (FLA-PA) and soluble TLR5 ectodomain (hTLR5-Fc) were from InvivoGen. Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: ... the cells are resuspended in FACS buffer containing 5ng/μL Fc-hDectin1 (Invivogen) and incubated in the dark on ice for 1 hour ...