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Citations for Invivogen :
1 - 50 of 226 citations for LAG 3 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Human Embryonic Kidney HEK293-Null2 cells (Cat #hkb-null2) and HEK293-hTLR4 cells (Cat #hkb-htlr4) were obtained from Invivogen and are isogenic reporter cell lines ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Immunology 2020Quote: HEK293 cells expressing murine TLR2 or human TLR4 and CD14/MD2 were purchased from InvivoGen. Cells were cultured in the presence of blasticidin (Nacalai Tesque ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR7 (Invivogen hkb-htlr7), HEK-Lucia™ hRIG-I (Invivogen hkl-hrigi) ...
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bioRxiv - Microbiology 2022Quote: ... HEK293-hTLR9 (Invivogen hkb-htlr9), the corresponding parental HEK293 null1 (InvivoGen hkb-null1) ...
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bioRxiv - Microbiology 2023Quote: HEK293-hTLR9 (Invivogen hkb-htlr9) and the corresponding parental HEK293 null1 (InvivoGen hkb-null1 ...
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bioRxiv - Immunology 2019Quote: ... 293/NOD2 cells were obtained by stable transfection of HEK293 cells with the pUNO-hNOD2 plasmid which expresses the human NOD2 gene (Invivogen). THP1 cells were obtained from ATCC.
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bioRxiv - Genomics 2020Quote: HEK293/hTLR4A-MD2-CD14 Cells (Invivogen) were selected for functional follow up of the IRF1 trans-eQTL because of the ease of transfection of HEK293 cells and the addition of the TLR4 receptor ...
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bioRxiv - Immunology 2021Quote: ... Hek293/hTLR4-HA cells (InvivoGen#293-htlr4ha) were a gift from Guang Yang (Jinan University).
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bioRxiv - Microbiology 2022Quote: HEK293 (HEK-Blue™ reporter cells, InvivoGen) -IFN-α/β and -IFN-λ cells were cultivated in DMEM (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... the corresponding parental HEK293 null1 (InvivoGen hkb-null1), HEK293-hTLR7 (Invivogen hkb-htlr7) ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the human hinge and IgG1 heavy chain Fc region (pFUSE-hIgG1-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Microbiology 2019Quote: Human TLR2/NF-κB/SEAP stably transfected HEK293 cells and human TLR4/MD-2/CD14/NF-κB/SEAP stably transfected HEK293 cells (Novus Biologicals, Centennial, CO, USA, currently sold by InvivoGen, San Diego, CA, USA) were maintained in DMEM with 4.5 g/L glucose ...
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bioRxiv - Microbiology 2023Quote: ... and the corresponding parental HEK293 null1 (InvivoGen hkb-null1) cell lines were grown in DMEM media supplemented with 10% heat-inactivated FBS and 1X GlutaMAX (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Cancer Biology 2022Quote: ... vector with the KIH strategy “knob” human Fc domain(54) MLA2-arm of the TriTE was converted into a scFab and cloned into a pFUSE (InvivoGen) vector with the KIH strategy “hole” human Fc domain followed by OKT3 ...
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bioRxiv - Microbiology 2020Quote: The DNA sequences of codon-optimized SARS-CoV-2 spike Receptor Binding Domain (S-RBD) and human ACE2 extracellular domain (hACE2-ECD) were cloned into a pFuse-Fc expression vector (Invivogen). A thrombin cleavage sequence was inserted between RBD and Fc to generate a cleavable human Fc tag for future studies ...
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bioRxiv - Immunology 2023Quote: ... The cDNAs were fused to the C terminus of human IgG1-Fc in the expression vector pFuse-hIgG1-Fc2 (InvivoGen). Constructs containing Clec12a or empty vector were transfected into Lx293 cells cultured with DMEM +10% FBS and Pen/strep (1mg/mL) ...
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bioRxiv - Biochemistry 2024Quote: ... a fragment of recombinant DNA encoding either residues 21 to 220 of human CD44 or 25 to 224 of murine CD44 were subcloned into an in-house Fc pFUSE-based vector (Invivogen, pfuse-hg1fc1) with a Gly/Ser linker in between ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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bioRxiv - Systems Biology 2020Quote: ... HEK293 or U2OS cells were treated with 10 μM MG132 (InvivoGen) at approximately 80% confluence for 4 h and successively harvested ...
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bioRxiv - Immunology 2022Quote: ... Reporter IFNγ (GAS) and ISG (IRF) HEK293 cell lines were purchased from Invivogen and expanded in DMEM supplemented with 10% heat inactivated FBS and selection antibiotics per vendor instructions.
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bioRxiv - Microbiology 2021Quote: ... 20 µl HEK293 culture supernatant was added to 180 µl QUANTI-Blue reagent (InvivoGen) and incubated at 37°C for 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-8xHis-AviTag plasmids were generated by subcloning the Spike-RBD or ACE2 DNA fragment into a pFUSE-hIgG1-Fc-AviTag vector (adapted from the pFUSE-hIgG1-Fc vector from InvivoGen). The ACE2-Fc-LgBiT fusion plasmids were generated by subcloning the gene fragments of LgBiT to the N- or C-terminus of the ACE2-TEV-Fc-AviTag vector with a 10-amino acid (N-terminal fusion ...
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bioRxiv - Bioengineering 2020Quote: ... VH-Fc were cloned into a pFUSE (InvivoGen) vector with a human IgG1 Fc domain ...
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bioRxiv - Immunology 2022Quote: ... 3′3′-cyclic-di-AMP (3′3′ CDA) (Invivogen, cat. no. tlrl-nacda), 2′3′-RR c-di-AMP (2′3′-RR-S2 CDA ...
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bioRxiv - Immunology 2023Quote: ... 10% FCS and 250µg/ml Hygromycin B gold (InvivoGen). Virus stock production was performed under BSL-3 conditions using DMEM medium supplemented with glutamax and 2% FCS ...
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bioRxiv - Immunology 2022Quote: ... ALPK1 knockout and TIFA knockout HEK293 cells and their parental cell line were purchased from Invivogen and termed HEK293 cells ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... 3′3′-cGAMP (100 μM; InvivoGen), c-di-AMP (100 µM ...
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bioRxiv - Immunology 2020Quote: ... + 1% FCS and treated with 500 ng/mL Pam3CSK4 (InvivoGen) for 4 hours and 10 μM nigericin (InvivoGen ...
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bioRxiv - Microbiology 2020Quote: ... All HEK293 Flp-In T-REx cell lines were additionally supplemented with 5 µg/mL Blasticidin S (Invivogen) and 200 µg/mL Hygromycin B (Invivogen ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen). For the generation of full length PSG1-Fc and PSG9-Fc ...
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bioRxiv - Cell Biology 2022Quote: ... 3- methyladenine (Invivogen), bafilomycin A1 (Invivogen) ...
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bioRxiv - Physiology 2022Quote: ... aeruginosa flagellin (FLA-PA) and soluble TLR5 ectodomain (hTLR5-Fc) were from InvivoGen. Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: ... the cells are resuspended in FACS buffer containing 5ng/μL Fc-hDectin1 (Invivogen) and incubated in the dark on ice for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and human IL-1β (Invivogen), were dissolved in PBS and added to the cell culture medium to achieve final concentrations of 10 μM (ADP-heptose) ...
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bioRxiv - Immunology 2023Quote: ... human p21 (Invivogen; 100 ng), human p27 (Invivogen ...
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bioRxiv - Immunology 2023Quote: ... human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng ...
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bioRxiv - Immunology 2023Quote: ... the human cDNA sequence (Invivogen) was subcloned into the pcDNA3 vector between the HindIII and EcoRV restriction sites followed by transfection of 5 µg plasmid into BAFF/CD40L double positive fibroblasts using Lipofectin reagent (Thermo) ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed twice with PBS and stained with Fc-hDectin-1a (Invivogen) and Calcofluor white (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... 2’,3’-cGAMP (Invivogen) at 10 μg/mL ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... we used engineered HEK293 cell lines (HEK-Blue) that stably express either mouse TLR9 (mTLR9) and an NF-κB reporter gene (Invivogen, San Diego, CA). In brief ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...