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Citations for Invivogen :
1 - 50 of 181 citations for Hepatitis E ORF2 protein mouse Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Anti-human IgG Fc Capture (AHC) Biosensors tips were initially loaded with Dectin-1A:FC fusion protein (Invivogen, #fc-hdec1a) at 13 ug/ml ...
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bioRxiv - Bioengineering 2020Quote: ... The Fc control protein was derived from the pFuse-IgG1e3-Fc2 (InvivoGen). For the generation of full length PSG1-Fc and PSG9-Fc ...
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bioRxiv - Microbiology 2019Quote: ... Mice were also subcutaneously immunized with 20 μg of Gn-Fc or Gc-Fc protein absorbed in aluminum hydroxychloride (Alhydrogel® adjuvant 2%, InvivoGen, Hong Kong). Mice sera were collected from immunized mice at one week after the third immunization to determine the levels of specific antibody titers ...
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bioRxiv - Microbiology 2020Quote: ... recombinant RBD protein containing a C-terminal 6xHis tag was formulated with the Alhydrogel adjuvant (Invivogen) and each vaccine dose contained 5 μg of RBD and 500 μg of aluminum hydroxide ...
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bioRxiv - Immunology 2021Quote: The capacity of E protein to activate NF-kB was tested by using HEK-TLR2-blue (InvivoGen). In this assay ...
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bioRxiv - Bioengineering 2022Quote: ... For FCM assay using hACE2-Fc (Invivogen, Catalogue code: fc-hace2), 5μL of the protein was incubated for 1 hour at 4°C with the cells using a similar assay condition described above ...
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bioRxiv - Bioengineering 2023Quote: ... fused with the cDNA sequence of the mouse hinge and IgG2a heavy chain Fc region (pFUSE-mIgG2a-Fc1, InvivoGen), downstream of a mouse Ig kappa chain secretion signal peptide and was subcloned into a pMSGV retroviral vector followed by enhanced (e)GFP reporter gene cassette using PCR and standard molecular cloning techniques to generate pMSGV-A4-Fc-T2A-eGFP ...
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bioRxiv - Microbiology 2023Quote: ... Soluble hACE2-Fc (fchace2, InvivoGen), a recombinant protein consisting of the extracellular domain of human ACE2 fused to a human IgG1 Fc region ...
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bioRxiv - Microbiology 2020Quote: ... three BALB/c mice were each injected intraperitoneally (i.p.) with 100 μg of RBD-Fc fusion protein formulated with 500 μg of aluminum hydroxide (Alhydrogel, Invivogen, USA) and 25 μg of CpG (Sangon ...
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bioRxiv - Immunology 2020Quote: ... LPS (from E. coli K12, Invivogen) was dissolved in RPMI and used at a final concentration of 100ng/mL ...
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bioRxiv - Microbiology 2020Quote: ... Soluble Fc-mDectin-1a containing the C-terminal extracellular domain of mouse Dectin-1a fused with the human IgG1 Fc domain was purchased from Invivogen. Overnight C ...
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bioRxiv - Neuroscience 2023Quote: ... with cMyc tag and 6xHis-tag at the nanobody’s C-terminal were cloned into a pFUSE-derived vector (InvivoGen). The vector was used to transform Expi293F mammalian cells (ThermoFisher) ...
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bioRxiv - Neuroscience 2020Quote: ... LPS (LPS from E. coli O111:B4) (Invivogen), polyinosinic:polycytidylic acid (Invivogen) ...
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bioRxiv - Biochemistry 2020Quote: ... ACE2-8xHis-AviTag plasmids were generated by subcloning the Spike-RBD or ACE2 DNA fragment into a pFUSE-hIgG1-Fc-AviTag vector (adapted from the pFUSE-hIgG1-Fc vector from InvivoGen). The ACE2-Fc-LgBiT fusion plasmids were generated by subcloning the gene fragments of LgBiT to the N- or C-terminus of the ACE2-TEV-Fc-AviTag vector with a 10-amino acid (N-terminal fusion ...
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bioRxiv - Bioengineering 2020Quote: ... VH-Fc were cloned into a pFUSE (InvivoGen) vector with a human IgG1 Fc domain ...
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bioRxiv - Immunology 2023Quote: ... 10% FCS and 250µg/ml Hygromycin B gold (InvivoGen). Virus stock production was performed under BSL-3 conditions using DMEM medium supplemented with glutamax and 2% FCS ...
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bioRxiv - Synthetic Biology 2023Quote: ... or HRP-conjugated anti-human IgG Fc (Invivogen; 31413). Proteins from transient transfections were either purified via His-tag or Protein A purification ...
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-conjugated anti-human IgG Fc antibodies (Invivogen; 31413) were used for detection ...
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bioRxiv - Immunology 2022Quote: ... Ultrapure LPS (E. coli 0111:B4) was purchased from Invivogen. MitoTEMPO and UK-5099 were purchased from Sigma Aldrich (SML0737 ...
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bioRxiv - Immunology 2020Quote: ... + 1% FCS and treated with 500 ng/mL Pam3CSK4 (InvivoGen) for 4 hours and 10 μM nigericin (InvivoGen ...
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bioRxiv - Synthetic Biology 2023Quote: ... 50uL of HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:5000 in blocking buffer was added to the plates and incubated at room temperature for 1hr ...
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bioRxiv - Microbiology 2021Quote: ... and LPS (100 ng / mL E. coli K12, k-eklps, Invivogen) with IFNγ (20 ng / mL ...
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bioRxiv - Immunology 2019Quote: ... or LPS (O111:B4, E. coli, 1µg/2,5×105 cells Invivogen) was achieved using FuGeneHD (Promega ...
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bioRxiv - Immunology 2023Quote: ... and LPS-EB (E. coli 0111:B4) were purchased from InvivoGen.
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bioRxiv - Immunology 2023Quote: ... group E mice were treated with dexamethasone (1 mg/kg, InvivoGen) intraperitoneally once daily from day 12 to 15 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Western blots using HRP-conjugated anti-human IgG Fc (Invivogen; 31413) diluted 1:10000 in blocking buffer ...
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bioRxiv - Biophysics 2023Quote: ... 1 μg/ml LPS (Ultrapure LPS from E. coli, 0111:B4, Invivogen) and 4 μM total monomer of sonicated Aβ fibrils were loaded into the nanopipette ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) for 6 hours ...
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bioRxiv - Immunology 2023Quote: ... and 1 ug/mL LPS (TLR4 agonist from E. coli O111:B4, InvivoGen)) and Brefeldin A for 6 hours in 96-well tissue culture plates at 37C in a 5% CO2 environment ...
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bioRxiv - Physiology 2022Quote: ... aeruginosa flagellin (FLA-PA) and soluble TLR5 ectodomain (hTLR5-Fc) were from InvivoGen. Dulbecco’s phosphate buffered saline (DPBS ...
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bioRxiv - Microbiology 2022Quote: ... the cells are resuspended in FACS buffer containing 5ng/μL Fc-hDectin1 (Invivogen) and incubated in the dark on ice for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed twice with PBS and stained with Fc-hDectin-1a (Invivogen) and Calcofluor white (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... PBMCs were rested and stimulated with 100 ng/ml LPS (from E. coli K12, Invivogen) or 0.1 μM CpG 2006 (TIB MOLBIOL) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were stimulated with ultrapure lipopolysaccaride (1 μg/mL, LPS, E. coli 0111:B4, InvivoGen), harvested and analyzed as described below at 0 ...
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bioRxiv - Cancer Biology 2020Quote: ... M25 scFv was subcloned into either the Fc fusion expression vector pFUSE-hIgG1 Fc2 (InvivoGen) or a modified Abvec expression vector that was originally provided by Dr ...
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bioRxiv - Immunology 2019Quote: ... into expression plasmids adapted from the pFUSE-rIgG-Fc and pFUSE2-CLIg-rK2 vectors (Invivogen). Human and rabbit Abs were transiently expressed with the FreeStyle 293 Expression System (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... indicated dose of conjugated or free SOSIP proteins (25 μg mi3-conjugated SOSIP per mouse in most experiments) were mixed with 20 μg MPLA (Invivogen, vac-mpls) and 10 μg Quil-A (Invivogen ...
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bioRxiv - Molecular Biology 2020Quote: ... received from Havard Medical school) and lipopolysaccharides (LPS, 1 µg/mL, E. coli 0111:B4, InvivoGen), respectively ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were activated with LPS (100 ng mL-1 E. coli O55:B5, triple-purified, InvivoGen) for 48 h ...
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bioRxiv - Immunology 2020Quote: ... USA). Ultrapure LPS (from E. coli O111:B4) was purchased from Invivogen (San Diego, CA, USA). Unless otherwise indicated ...
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bioRxiv - Neuroscience 2023Quote: ... microglia cells were first primed with 100 ng/ml ultrapure LPS (E. coli 0111:B4, Invivogen) and then incubated at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Mature-DC were induced by adding LPS (200 ng/ml; E. coli K12; Invivogen, San Diego, CA) in the last 18h of culture ...
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bioRxiv - Immunology 2021Quote: ... TLR4 activation was stimulated by adding lipopolysaccharide (LPS-EB from E. coli 0111:B4; InvivoGen, Toulouse, France) in a final concentration of 50 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... moDCs were stimulated with 100 ng/mL ultrapure LPS (E. coli 0111 B4 strain, InvivoGen, San Diego) or left untreated ...
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bioRxiv - Immunology 2022Quote: ... mouse TLR2 neutralizing antibody monoclonal mouse IgG2a (C9A12) and control isotypes mouse IgG2a were from InvivoGen. Mouse TLR4/MD2 complex neutralizing antibody monoclonal rat IgGk clone MTS510 and control isotypes rat IgGk were from eBioscience™ Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Biophysics 2019Quote: The coding sequences of antibody Fc heterodimerization domains were amplified from the pFUSE-hIgG1-Fc1 plasmid (Invivogen) containing two charged mutations on each opposing domain57,58 ...
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bioRxiv - Immunology 2019Quote: ... before being cultured for indicated time points in FCS-free medium containing 1 µg/ml LPS (Invivogen), 1 μg/ml synthetic (B ...
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bioRxiv - Immunology 2022Quote: ... Cells were seeded at 2.5 × 105 cells/well in 24-well tissue-culture plates, treated with EtOH (1.69%, or 0.83%) 10 ng/mL LPS (Ultrapure LPS, E. coli 0111:B4, Invivogen), 500 μM or 1 mM palmitic acid (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: ... injection with10 mg/kg in a total volume of 100μl of LPS (E. coli 055:B5 ultrapure; Invivogen). Mice were culled after 90 mins and serum collected via cardiac puncture for measurement of serum cytokines.